Development of graphene oxide-based fluorescent sensing nanoplatform for microRNA-10b detection

Maroua Moslah, K. Djebbi, C. Tlili, C. Dridi, Deqiang Wang
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Abstract

Herein, we develop a fluorescent sensor for simple detection of miRNA-10b, which combines the fluorescence quenching ability of graphene oxide (GO) and the duplex-specific nuclease (DSN) mediated target recycling amplification. Our sensor exhibits desirable sensitivity for miRNA-10b with a 530 fM detection limit that could be achieved within 75 min. Furthermore, our sensor showed good selectivity for discriminating target miRNA and other microRNAs.
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基于氧化石墨烯的微rna -10b荧光传感纳米平台的研制
在此,我们开发了一种用于简单检测miRNA-10b的荧光传感器,该传感器结合了氧化石墨烯(GO)的荧光猝灭能力和双特异性核酸酶(DSN)介导的靶循环扩增。我们的传感器对miRNA-10b具有理想的灵敏度,在75分钟内可达到530 fM的检测限。此外,我们的传感器对区分靶miRNA和其他microrna具有良好的选择性。
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