Study on the Effect and Simulated Gastrointestinal Digestion of Polysaccharide Extracted from Ginseng Berry in Vitro

Proceedings of the 6th International Conference on Biomedical Engineering and Applications Pub Date : 2022-05-13 DOI:10.1145/3543081.3543103

Yanru Chen, Tianyu Yin, Hang Cui, Miao Wang, T. Jin, Fushun Cui

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Abstract

The physiological activity of ginseng berry polysaccharide (WGBP) extracted by ultrasound and antioxidant activity during simulated gastrointestinal digestion in vitro was studied. The antioxidant activity of WGBP in vitro was studied by DPPH•, ABTS•, •OH scavenging method, and its inhibitory effect on α-glucosidase was analyzed, and antioxidant activity retention rate of WGBP was evaluated by in vitro simulated gas-trointestinal digestion. The results showed that WGBP had strong ability to scavenge DPPH•, ABTS•, and •OH. In the simulation of in vitro gastrointestinal digestion, the retention rates of DPPH•, ABTS•, and •OH scavenging capacity of 5.00 mg/mL WGBP were high after the WGBP was digested for 1 h in the simulated gastric fluid and 2 h in the simulated gastrointesti-nal fluid, which indicated that it still had strong antioxidant activity. In conclusion, WGBP has strong in vitro efficacy, and still has strong antioxidant activity after simulated digestion in vitro.

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人参浆果多糖体外作用及模拟胃肠消化的研究

研究了超声提取人参莓多糖(WGBP)在体外模拟胃肠消化过程中的生理活性和抗氧化活性。采用DPPH•、ABTS•、•OH清除率法研究WGBP的体外抗氧化活性，分析其对α-葡萄糖苷酶的抑制作用，并采用体外模拟气-肠消化法评价WGBP的抗氧化活性保留率。结果表明，WGBP对DPPH•、ABTS•和•OH具有较强的清除能力。在体外胃肠消化模拟实验中，WGBP在模拟胃液中消化1 h、在模拟胃液中消化2 h后，5.00 mg/mL WGBP的DPPH•、ABTS•和•OH清除能力均较高，表明其仍具有较强的抗氧化活性。综上所述，WGBP具有较强的体外功效，体外模拟消化后仍具有较强的抗氧化活性。

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Proceedings of the 6th International Conference on Biomedical Engineering and Applications

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