Synchronized High-Content Recording of Cardiomyocytes in Vitro by Integrated Cell-Based Biosensor

Abstract

Quantitative and chronic monitoring of biological and physiological status of cardiomyocytes plays a significant role in building in vitro cardiac models for the study of cardiology and pharmacology. Label-based recording methods usually cause damage to cells and fail to perform the accurate and chronic recording of cardiomyocytes. Here, we develop an integrated microelectrode and interdigitated electrode multifunctional biosensor, simultaneously recording the extracellular potential, mechanical beating and growth viability signals of cardiomyocytes in a long-term, dynamic, real-time, and non-invasive manners. By further analysis of the multiparameter signals, it can be concluded that cardiomyocytes will reach stable status after eight-day culture, which could be employed for drug screening. We believe our multifunctional biosensing system will be a utility platform for the study of cardiology and pharmacology.