Flow injection system for real-time monitoring of lactic acid at the outer surface of the skin

H. Kudo, Keigo Enomoto, Yota Jingushi
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引用次数: 2

Abstract

A flow injection system using electrochemical biosensor for in-time monitoring of lactic acid at the skin's surface was fabricated and tested. The system consists of a microfluidic `skin-patch' sampling device and an electrochemical biosensor using a carbon graphite electrode. In order to enhance the stability of monitoring, lactic acid excreted at the skin's surface was directly solved into the career flow (phosphate buffered saline) in the `skin-patch' sampling device. Therefore our system measures the amount of lactic acid secretion instead of concentrations of lactic acid in the sweat. The electrodes of the biosensor was modified using osmium wired horseradish peroxidase (Os-HRP) redox polymer. The detection limit of the biosensor for lactic acid was 1.0 μM and the sensitivity was 3.125 μA / mM at 1.0 mM. Real-time monitoring of excreted lactic acid during the stationary bike workout was carried out. The flow injection system was attached on the upper arm of the volunteer. Owing to the `skin-patch' flow-channel, transudation of lactic acid was successfully monitored continuously in real-time with sufficient stability. As a result, the lactic acid level increased during the exercise and decreased to the initial level after the exercise. From this result, the system is expected to be used in sports science field such as scientific sports training.
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流动注射系统,用于实时监测皮肤外表面的乳酸
研制了一种用于皮肤表面乳酸实时监测的电化学生物传感器流动注射系统。该系统由一个微流体“皮肤贴片”采样装置和一个使用碳石墨电极的电化学生物传感器组成。为了增强监测的稳定性,在“皮肤贴片”取样装置中,将皮肤表面排泄的乳酸直接溶解到职业流(磷酸盐缓冲盐水)中。因此,我们的系统测量的是乳酸分泌量,而不是汗液中乳酸的浓度。该生物传感器电极采用锇线辣根过氧化物酶(Os-HRP)氧化还原聚合物修饰。乳酸传感器的检出限为1.0 μM,灵敏度为3.125 μA / mM (1.0 mM),实时监测运动过程中乳酸的排泄量。流动注射系统安装在志愿者的上臂上。由于“皮肤贴片”流动通道,乳酸的转化成功地连续实时监测,具有足够的稳定性。因此,乳酸水平在运动过程中升高,运动后下降到初始水平。从实验结果来看,该系统有望应用于科学运动训练等体育科学领域。
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