A three-dimensional microfluidic device for oocyte zona-removal and incubation

Chuan Chang, Yueh-Jen Chen, L. Pan, F. Tseng
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Abstract

To solve the low implantation rate of in-vitro fertilization (IVF), zona-free oocyte is widely used in IVF. However the process of zona-removal is not only tedious but also easy to harm the fragile oocyte. In this work we introduce a 3-dimensional microfluidic device to simplify the zona-removal process, and the applied gentle process is also capable to keep oocyte intact and precisely locate all oocytes in the well throughout the procedure.
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一种用于卵母细胞带去除和培养的三维微流控装置
为解决体外受精着床率低的问题,无带卵母细胞被广泛应用于体外受精。但除带过程繁琐且易损伤脆弱的卵母细胞。在这项工作中,我们引入了一个三维微流体装置来简化带去除过程,并且应用的温和过程也能够保持卵母细胞完整并在整个过程中精确定位孔中的所有卵母细胞。
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