Electroporation system generating wide range square-wave pulses for biological applications

V. Stankevič, V. Novickij, S. Balevičius, N. Žurauskienė, A. Baskys, A. Dervinis, V. Bleizgys
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引用次数: 11

Abstract

The design of a wide range square-wave pulse electroporation system is presented. The system is able to generate pulses having voltage amplitudes up to 4000 V and currents up to 100 A. The quantity of accumulated energy is optimized by the use of the variable capacitor bank. The proposed device has an integrated graphic LCD and measurement modules for current pulse visualization, and does not require an external oscilloscope. The whole system was tested with suspension of human lymphoma cells of Jurkat line in RPMI medium under a variety of electroporation conditions.
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电穿孔系统产生宽范围的方波脉冲的生物应用
介绍了一种宽范围方波脉冲电穿孔系统的设计。该系统能够产生电压幅值高达4000 V、电流高达100 A的脉冲。利用可变电容器组优化了蓄能量。该装置具有集成的图形LCD和测量模块,用于电流脉冲可视化,并且不需要外部示波器。将Jurkat系人淋巴瘤细胞悬液置于RPMI培养基中,在多种电穿孔条件下对整个体系进行测试。
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