{"title":"Transformation of Escherichia coli by R404 factor DNA and properties of the transformants.","authors":"E Kurowska, S Cebrat, T M Lachowicz","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Three r-determinants (Cb Cm Cf) out of eight present in R404 factor were transferred in transformation of Escherichia coli by the plasmid DNA isolated from minicells. The r-determinants acquired in this way formed the replicon tr404-1 and were untransferable by conjugation unless another self-transmissible plasmid was present in recipient. The evidences are presented in support of hypothesis that the transfer events consist in infection of transformant cells with sex factor, recombination between tr404-1 replicon and the sex factor and finally transfer of the new formed structure to the host cells of the sex factor.</p>","PeriodicalId":75389,"journal":{"name":"Acta microbiologica Polonica. Series A: Microbiologia generalis","volume":"8 1","pages":"35-42"},"PeriodicalIF":0.0000,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Polonica. Series A: Microbiologia generalis","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Three r-determinants (Cb Cm Cf) out of eight present in R404 factor were transferred in transformation of Escherichia coli by the plasmid DNA isolated from minicells. The r-determinants acquired in this way formed the replicon tr404-1 and were untransferable by conjugation unless another self-transmissible plasmid was present in recipient. The evidences are presented in support of hypothesis that the transfer events consist in infection of transformant cells with sex factor, recombination between tr404-1 replicon and the sex factor and finally transfer of the new formed structure to the host cells of the sex factor.
R404因子中存在的8个r-决定因子中有3个(Cb Cm Cf)在大肠杆菌转化中被从微细胞中分离的质粒DNA转移。以这种方式获得的r决定子形成复制子tr404-1，除非在受体中存在另一个自传质粒，否则不能通过接合转移。这些证据支持了tr404-1复制子与性因子的重组，并最终将新形成的结构转移到性因子的宿主细胞中的假设。
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