Effect of sera on transfection Bacillus subtilis.

R Staniewski
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引用次数: 0

Abstract

The effect of normal human, rabbit and cow sera, SP50 phage antiserum and the albumin and globulin fractions of normal rabbit serum on transfection of Bacillus subtilis 168 ind was studied. All the sera reduced to varying degrees the efficiency of the process within 60 min. from infection of the culture. None of the sera, except for the antiphage serum, influenced the further course of the process. The albumin fraction of normal rabbit serum otherwise than the globulin fraction, was found to stimulate transfection. The effect of the sera on transfection was influenced by the composition of the media.

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血清对枯草芽孢杆菌转染的影响。
研究了正常人、兔、牛血清、SP50噬菌体抗血清和正常兔血清白蛋白、球蛋白部分对枯草芽孢杆菌168 - ind转染的影响。所有血清在感染培养物后60分钟内都不同程度地降低了处理效率。除抗噬菌体血清外,其他血清均不影响该过程的进一步发展。正常兔血清中的白蛋白部分比球蛋白部分更能刺激转染。血清对转染的影响受培养基组成的影响。
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