Protective effect of B.clasuii on sepsis

Abstract

DOI: 10.4328/AEMED.127 Received: 15.03.2018 Accepted: 24.03.2018 Published Online: 26.03.2018 Printed Online: 01.05.2018 J Ann Eu Med 2018;6(2): 20-3 Corresponding Author: Varlik K. Erel, Department of Anesthesiology and Reanimation, Adnan Menderes University, School of Medicine, Aydın, Turkey. E-Mail: varlik.erel@gmail.com ORCID ID: 0000-0002-0539-661X Abstract Aim: Sepsis is a clinical problem that still has high mortality and morbidity despite advances in supportive care in intensive care. The study’s aim was to investigate the protective effect of Bacillus clausii, an important probiotic with a strengthening effect on the gastrointestinal barrier, on septicemia. Material and Method: Twenty-four rats were divided into three randomized and double-blind groups, as Group 1 (control group, n=8), Group 2 (sepsis group, n=8), and Group 3 (sepsis+probiotic group, n=8). Lipopolysaccharide extracted from serotypes (0111:B4) of Escherichia coli (E.coli ) was injected intraperitoneally to all group for sepsis model at a dose of 15 mg/kg and rats were sacrificed at 48 hours. Serum oxidant and antioxidant parameters were measured. Results: The C-Reactive Protein (CRP) value of Group 1 was significantly lower than Group 2 [CI:(-44.16)-(-28.52), p=0.000], whereas the CRP values of Group 3 were significantly lower than Group 2 [CI:(7.4)-(34.7), p=0.005]. MDA values were found to be significant between Groups 1 and 2, CI:(-0.3)-(-0.7), p=0.045. The thiol values of Group 2 were significantly lower than Group 1 [CI:(9)-(366), p=0.041], whereas the thiol (SH) values of Group 3 were significantly higher than those of Group 2 [CI:(-659)-(-98), p=0.016]. Discussion: B. clausii is one of a group of probiotics that is inexpensive and effective. Sepsis is still a high-risk clinical problem. Our study showed that B. clausii has a protective effect against sepsis.