A nano-gap biosensor using nano-patterned conductive molecule for cTnT detection

Abstract

Nanostructure has been envisioned as a novel factor to enhance biomolecular sensing characteristics. In this work, we propose a novel biosensor by using a nano gap formed between two electrodes for biomolecular detections. The nano-gap electrode increases sensitivity of near-surface electrochemical conductances. To examine the proposed sensing characteristics of the nano-gap electrode, different conductive linkers (CB2C and PABA were immobilized to form a conductive layer on the nano-gap surface between the electrodes. The conductance increment of CB2C and PABA linker was about 56% and 396% respectively. This conductance improvement provides nano-gap electrodes ability for cTnT detection, also, the dynamic detection range of CB2C and PABA is from 1ng/ml to 100ng/ml and from 10pg/ml to 100ng/ml respectively. The results also proved that the thickness of conductive layer is a critical factor in conductance and detection limit.