{"title":"[Variability of parameters in enzyme kinetics. 1. Intermethod oscillations].","authors":"B C de Almeida Cunha, M Vitolo","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Kinetic parameters of urease activity have been determined by five different methods, namely: Henry, Lineweaver-Burk, Hofstee, Woolf and Cornish-Eisenthal. In no instance different methods yielded the same values for the set of parameters (Km and Vmáx).</p>","PeriodicalId":76437,"journal":{"name":"Revista de farmacia e bioquimica da Universidade de Sao Paulo","volume":"14 1","pages":"23-34"},"PeriodicalIF":0.0000,"publicationDate":"1976-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Revista de farmacia e bioquimica da Universidade de Sao Paulo","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Kinetic parameters of urease activity have been determined by five different methods, namely: Henry, Lineweaver-Burk, Hofstee, Woolf and Cornish-Eisenthal. In no instance different methods yielded the same values for the set of parameters (Km and Vmáx).
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