The authors showed that the colorimetric methods used to effect aminoacidopathies screening are inadequate and unspecific. The thin layer chromatography was chosen to effect such screening and the authors showed the incidence in newborns of some aminoacid metabolism abnormalities.
This investigation was performed to study the effect of lactose on the fixation of calcium in bones of rats. Lactose 3% and 10% concentration was used and the action was compared to that obtained by 67 U.I. of vitamin D in 100 g diet. The data obtained indicated very definitely that the presence of lactose in the diet favorably influences the fixation of Ca in bone, when the amount is 10%; at 3% concentration lactose don't seem influence Ca fixation in bone.
The authors observed the influence of the B1 aflatoxin over rat liver and kidneys glucose-6-phosphatase activity. Animals aged 30 and 60 days received B1 aflatoxin in oil, 1 mcg/g and 5 mcg/g of body weight. The parameters were observed 6, 24 and 48 hours after the micotoxin administration. There was significant decrease of glucemia in the 30 days animals. In the 60 days animals the results suggested possible increase in the liver glucose-6-phosphatase and marked decrease in glycogen.
In this first of three parts, operational parameters (power consumption with and without aeration, aeration number, oxygen volumetric transfer coefficient and hold up) of the system used are presented, together with graphs and tables to show the relation between these parameters and controlable operating variables. Later the values here presented will be correlated with KLa determined by SOM, the kinetics of oxygen transfer in yeast cultures.
In order to improve the identification and quantity determination of chromium in biological material the conditions for obtaining chromium chelate were studied. The method used for this purpose was gas chromatography standardized by isotopic dilution (Cr-51) technique. The results showed the adequacy of the method concerning sensitivity and standardization.
By CNDO (Complete Neglect of Differential Overlap) molecular orbital method, interatomic distances and XYZ cartesian corrdinates were calculated in five polymorphs (monohydrated, alpha, two beta, and gamma) of sulfanilamide. Interatomic distances thus obtained are very close to those originally presented by Bells & Roblin and support the mechanism of action postulated long algo for sulfa drugs as being competitive antagonism with p-aminobenzoic acid.
The stability of tetracycline was studied at pH 6.0 and 1.5. The thermal inactivation constant at the lower pH was found to vary between 1.62 min-1 (40 degrees C) and 65.83 min-1 (100 degrees C). A degradation mechanism was proposed in the light of the variation of entropy and steric factors.
The isolation of protein fractions in cocont water was achieved by precipitation with controlled pH variation obtaining three isolates at pH 8,5, 10,5, and 11,5. By comparing each of these isolates with proteins of coconut milk, a similarity between properties of the first two isolates of the water - pH 8,5, 10,5--and those of coconut serum proteins -- glutelin and prolamin was observed. The third isolate is entirely absent from the milk, when coconut water is not used in the second pressing to obtain the milk.
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