[The influence of zoledronic acid on proliferation, migration and apoptosis of fibroblasts].

Chinese Journal of Oral Implantology Pub Date : 2019-10-01 DOI:10.19439/J.SJOS.2019.05.008

Lang Miao-jie, OU Yue-jian

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Abstract

PURPOSE: Bisphosphonates are commonly used to treat bone-derived malignant tumors currently. In recent years, with the widespread use of these drugs, reports on the difficulty of wound healing (DWH) increase remarkably. However, the mechanism is still unclear. The soft tissue injury was suspected to cause DWH in many recent reports. This experiment was designed to explore the influence of zoledronic acid on fibroblast, and investigate the possible mechanisms leading to DWH. METHODS: NIH 3T3 fibroblasts were routinely cultured in vitro. The influence of zoledronic acid on apoptosis, proliferation and migration of fibroblast were tested with flow cytometry, MTT and cell migration assay, respectively. The results of each experimental group were compared with the control group using SPSS 17.0 software package. RESULTS: At a concentration of 5-50 μmol/L, zoledronic acid could dose-dependently inhibit the activity of fibroblast proliferation and migration, and up-regulate apoptosis with significant difference (P<0.05). CONCLUSIONS: Zoledronic acid can up-regulate cell apoptosis, inhibit proliferation and migration of fibroblast, suggesting that soft tissue impairment might contribute to the occurrence of DWH.

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唑来膦酸对成纤维细胞增殖、迁移和凋亡的影响。

目的:双膦酸盐目前被广泛用于骨源性恶性肿瘤的治疗。近年来，随着这些药物的广泛使用，关于伤口愈合困难(DWH)的报道显著增加。然而，其机制尚不清楚。在最近的许多报道中，软组织损伤被怀疑是导致DWH的原因。本实验旨在探讨唑来膦酸对成纤维细胞的影响，并探讨导致DWH的可能机制。方法:体外常规培养NIH 3T3成纤维细胞。采用流式细胞术、MTT法和细胞迁移法分别检测唑来膦酸对成纤维细胞凋亡、增殖和迁移的影响。采用SPSS 17.0软件包对各实验组的结果与对照组进行比较。结果:唑来膦酸在5 ~ 50 μmol/L浓度下能呈剂量依赖性抑制成纤维细胞增殖和迁移活性，上调细胞凋亡，差异有统计学意义(P<0.05)。结论:唑来膦酸可上调细胞凋亡，抑制成纤维细胞增殖和迁移，提示软组织损伤可能与DWH的发生有关。

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Chinese Journal of Oral Implantology

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