Pub Date : 2020-04-01DOI: 10.19439/J.SJOS.2020.02.006
Wang Xue-mei, J. Yong
PURPOSE To investigate the effect of disulfiram on epithelial-mesenchymal transformation(EMT) in oral squamous cell carcinoma(OSCC) cells based on Smad4 mutation. METHODS The cancer tissues and adjacent normal tissues of 46 patients with OSCC who underwent tumor resection in the Affiliated Stomatological Hospital of Anhui Medical University from June 2017 to June 2018 were included in the study. Immunohistochemical staining was used to observe the expression of Smad4 in tissues, Western blot was used to determine the expression of Smad4 in tumor cells. The effects of disulfiram on TGF-β1-induced cell EMT migration, invasion, morphology and expression of p38, JNK and ERK were analyzed. SPSS 20.0 software package was used to analyze the data. RESULTS The positive expression rate of Smad4 in cancer tissues was significantly lower than that in adjacent normal tissues (P 0.05). The survival rate of human tongue cancer SCC-25 and CAL-27 cells was significantly lower than that of the control group at disulfiram concentrations ≥30 μmol/L(P<0.05). After treatment with TGF-β1, the morphology of SCC-25 and CAL-27 cells changed from epithelial to mesenchymal. E-cadherin expression was significantly reduced, Vimentin and Snail expression were significantly increased, and migration and invasion were enhanced (P<0.05). After disulfiram + TGF-β1 treatment, as the concentration of disulfiram increased, the morphological changes of SCC-25 and CAL-27 cells gradually decreased, the expression of E-cadherin protein gradually increased, the expression of Vimentin and Snail protein gradually decreased, and migration ability gradually weakened (P<0.05). After 5 minutes of TGF-β1 stimulation, p-ERK levels in SCC-25 and CAL-27 cells gradually increased, reached a maximum at 15 minutes, and then gradually decreased (P<0.05). After 20 μmol/L disulfiram + TGF-β1 treatment, p-ERK levels in SCC-25 and CAL-27 cells gradually decreased with the increase of the treatment time(P<0.05). CONCLUSIONS Disulfiram can inhibit EMT of Smad4 mutant and Smad4 non-mutated OSCC cells by blocking ERK phosphorylation in the MAPK signaling pathway.
{"title":"[Effect of disulfiram on epithelial-mesenchymal transformation of oral squamous cell carcinoma cells].","authors":"Wang Xue-mei, J. Yong","doi":"10.19439/J.SJOS.2020.02.006","DOIUrl":"https://doi.org/10.19439/J.SJOS.2020.02.006","url":null,"abstract":"PURPOSE To investigate the effect of disulfiram on epithelial-mesenchymal transformation(EMT) in oral squamous cell carcinoma(OSCC) cells based on Smad4 mutation. METHODS The cancer tissues and adjacent normal tissues of 46 patients with OSCC who underwent tumor resection in the Affiliated Stomatological Hospital of Anhui Medical University from June 2017 to June 2018 were included in the study. Immunohistochemical staining was used to observe the expression of Smad4 in tissues, Western blot was used to determine the expression of Smad4 in tumor cells. The effects of disulfiram on TGF-β1-induced cell EMT migration, invasion, morphology and expression of p38, JNK and ERK were analyzed. SPSS 20.0 software package was used to analyze the data. RESULTS The positive expression rate of Smad4 in cancer tissues was significantly lower than that in adjacent normal tissues (P 0.05). The survival rate of human tongue cancer SCC-25 and CAL-27 cells was significantly lower than that of the control group at disulfiram concentrations ≥30 μmol/L(P<0.05). After treatment with TGF-β1, the morphology of SCC-25 and CAL-27 cells changed from epithelial to mesenchymal. E-cadherin expression was significantly reduced, Vimentin and Snail expression were significantly increased, and migration and invasion were enhanced (P<0.05). After disulfiram + TGF-β1 treatment, as the concentration of disulfiram increased, the morphological changes of SCC-25 and CAL-27 cells gradually decreased, the expression of E-cadherin protein gradually increased, the expression of Vimentin and Snail protein gradually decreased, and migration ability gradually weakened (P<0.05). After 5 minutes of TGF-β1 stimulation, p-ERK levels in SCC-25 and CAL-27 cells gradually increased, reached a maximum at 15 minutes, and then gradually decreased (P<0.05). After 20 μmol/L disulfiram + TGF-β1 treatment, p-ERK levels in SCC-25 and CAL-27 cells gradually decreased with the increase of the treatment time(P<0.05). CONCLUSIONS Disulfiram can inhibit EMT of Smad4 mutant and Smad4 non-mutated OSCC cells by blocking ERK phosphorylation in the MAPK signaling pathway.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131105373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.19439/J.SJOS.2019.05.017
Ding Hong-zhong, XU Cong-ping, Zhou Yong-min
PURPOSE To investigate the independent prognostic factors and establish survival prediction model for advanced oral squamous cell carcinoma (OSCC). METHODS At the time of admission, the general information and past medical history of 177 patients with advanced OSCC were collected in detail. All patients underwent radical resection. After surgery, radiotherapy and chemotherapy with hydroxycampothecin (HCPT) and pingyangmycin (PYM) were performed according to the patient's condition. All patients were followed up for 5 years after surgery, and the survival rate during that period and the risk factors for prognosis were analyzed. The data were analyzed by SPSS 20.0 software package. RESULTS During the follow-up period, 2 patients died due to other causes, 4 were excluded due to lost to follow-up, 42 of the remaining 111 patients died, 69 survived, the total survival rate was 62.16%. The survival status of patients with advanced OSCC with different age, T stage and M stage had no significant difference (P>0.05), while significant difference was found among patients with different gender, tissue differentiation degree, N stage and chemotherapy (P 0.05). The survival curves of gender, N stage and HCPT in combination of PYM chemotherapy had certain predictive value on the survival of patients with advanced OSCC. CONCLUSIONS Gender, N stage and HCPT in combination of PYM chemotherapy are independent risk factors for the survival of patients with advanced OSCC, and their survival curves can be used as a survival prediction model.
{"title":"Independent prognostic factors and survival prediction model for 117 patients with advanced oral squamous cell carcinoma","authors":"Ding Hong-zhong, XU Cong-ping, Zhou Yong-min","doi":"10.19439/J.SJOS.2019.05.017","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.05.017","url":null,"abstract":"PURPOSE To investigate the independent prognostic factors and establish survival prediction model for advanced oral squamous cell carcinoma (OSCC). METHODS At the time of admission, the general information and past medical history of 177 patients with advanced OSCC were collected in detail. All patients underwent radical resection. After surgery, radiotherapy and chemotherapy with hydroxycampothecin (HCPT) and pingyangmycin (PYM) were performed according to the patient's condition. All patients were followed up for 5 years after surgery, and the survival rate during that period and the risk factors for prognosis were analyzed. The data were analyzed by SPSS 20.0 software package. RESULTS During the follow-up period, 2 patients died due to other causes, 4 were excluded due to lost to follow-up, 42 of the remaining 111 patients died, 69 survived, the total survival rate was 62.16%. The survival status of patients with advanced OSCC with different age, T stage and M stage had no significant difference (P>0.05), while significant difference was found among patients with different gender, tissue differentiation degree, N stage and chemotherapy (P 0.05). The survival curves of gender, N stage and HCPT in combination of PYM chemotherapy had certain predictive value on the survival of patients with advanced OSCC. CONCLUSIONS Gender, N stage and HCPT in combination of PYM chemotherapy are independent risk factors for the survival of patients with advanced OSCC, and their survival curves can be used as a survival prediction model.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116977778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-10-01DOI: 10.19439/J.SJOS.2019.05.020
Xiao Ling, Chen Rui-xia, Luo Na
PURPOSE: To investigate the effect of single low level laser therapy on initial pain during fixed orthodontic treatment. METHODS: Sixty patients who underwent fixed orthodontic treatment in our hospital from January 2015 to December 2017 were enrolled.Patients were divided into 2 groups by random number table method, 30 in each group. Patients in the experimental group were treated with a single low level laser therapy ,while patients in the control group were treated with a placebo.The results of the 2 groups were compared in regard to spontaneous pain and chewing pain during the day and night using SPSS 22.0 software package. RESULTS: Spontaneous pain during day and night with 0.012, 0.014 inch superelastic nickel-titanium (NiTi) line was significantly lower in the experimental group than in the control group(P 0.05).Chewing pain with 0.012, 0.014, 0.016, and 0.018 inch NiTi lines in the experimental group was significantly lower than that in the control group(P<0.05). CONCLUSIONS: Single low level laser therapy significantly reduced postoperative pain while placing a superelastic NiTi wire for initial alignment and correction.
{"title":"Effect of single low level laser therapy on initial pain during fixed orthodontic treatment","authors":"Xiao Ling, Chen Rui-xia, Luo Na","doi":"10.19439/J.SJOS.2019.05.020","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.05.020","url":null,"abstract":"PURPOSE: To investigate the effect of single low level laser therapy on initial pain during fixed orthodontic treatment. METHODS: Sixty patients who underwent fixed orthodontic treatment in our hospital from January 2015 to December 2017 were enrolled.Patients were divided into 2 groups by random number table method, 30 in each group. Patients in the experimental group were treated with a single low level laser therapy ,while patients in the control group were treated with a placebo.The results of the 2 groups were compared in regard to spontaneous pain and chewing pain during the day and night using SPSS 22.0 software package. RESULTS: Spontaneous pain during day and night with 0.012, 0.014 inch superelastic nickel-titanium (NiTi) line was significantly lower in the experimental group than in the control group(P 0.05).Chewing pain with 0.012, 0.014, 0.016, and 0.018 inch NiTi lines in the experimental group was significantly lower than that in the control group(P<0.05). CONCLUSIONS: Single low level laser therapy significantly reduced postoperative pain while placing a superelastic NiTi wire for initial alignment and correction.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126506442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-08-01DOI: 10.19439/J.SJOS.2019.04.013
Zheng Chong-yang, Zhang Xin-yu, C. Rui, Liu Liu, Hu Yong-jie
PURPOSE To investigate the clinical outcomes and prognosis of harmonic scalpel assisted extracapsular dissection for benign parotid tumors. METHODS A total of 132 patients with full medical archives who were diagnosed as benign parotid tumors from 3 medical centers were retrospectively analyzed. The included patients were divided into 3 groups according to tumor positions, namely, superficial pre-auricular, superficial tail, and deep lobe. The intra- and peri-operative variables, complications, functional outcomes and recurrence rates were analyzed with SPSS 16.0 software package. RESULTS The surgical and drainage time were longer and the blood loss and drainage amount were significantly greater in deep lobe group (P 0.05). More patients with decreased sensation or sialocele were observed in deep lobe group (P=0.004, 0.043, respectively), and no significant differences were found for wound infection and Frey's syndrome among 3 groups (P>0.05).Transient facial nerve paralysis occurred more frequently in deep lobe group (P<0.01), while permanent facial nerve paralysis was similar (P=0.052). No significant difference was observed for tumor recurrence (P=1.000). CONCLUSIONS HS-ECD displays good outcomes and prognosis for benign tumor of parotid gland, with improved esthetic and functional results as well as fewer complications and recurrence rate postoperatively.
{"title":"Evaluation of harmonic scalpel assisted extracapsular dissection for benign parotid tumors","authors":"Zheng Chong-yang, Zhang Xin-yu, C. Rui, Liu Liu, Hu Yong-jie","doi":"10.19439/J.SJOS.2019.04.013","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.04.013","url":null,"abstract":"PURPOSE To investigate the clinical outcomes and prognosis of harmonic scalpel assisted extracapsular dissection for benign parotid tumors. METHODS A total of 132 patients with full medical archives who were diagnosed as benign parotid tumors from 3 medical centers were retrospectively analyzed. The included patients were divided into 3 groups according to tumor positions, namely, superficial pre-auricular, superficial tail, and deep lobe. The intra- and peri-operative variables, complications, functional outcomes and recurrence rates were analyzed with SPSS 16.0 software package. RESULTS The surgical and drainage time were longer and the blood loss and drainage amount were significantly greater in deep lobe group (P 0.05). More patients with decreased sensation or sialocele were observed in deep lobe group (P=0.004, 0.043, respectively), and no significant differences were found for wound infection and Frey's syndrome among 3 groups (P>0.05).Transient facial nerve paralysis occurred more frequently in deep lobe group (P<0.01), while permanent facial nerve paralysis was similar (P=0.052). No significant difference was observed for tumor recurrence (P=1.000). CONCLUSIONS HS-ECD displays good outcomes and prognosis for benign tumor of parotid gland, with improved esthetic and functional results as well as fewer complications and recurrence rate postoperatively.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123579834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19439/J.SJOS.2019.03.018
Liu Wei-dong, Zhao Min
PURPOSE: To investigate the clinical effect and safety of modified Xiaoyao pill on the treatment of oral lichen planus (OLP) patients with anxiety or depression. METHODS: Sixty OLP patients with anxiety and depression were randomly divided into 2 groups, treated separately with hydroxychloroquine(HCQ, control group) and HCQ+modified Xiaoyao pill(experimental group). The results were measured with semi quantitative reticular erosive and ulcerative lesion (REU) and visual analogue scale（VAS） scoring system at the first visit, the second week, the fourth week and the eighth week, respectively. Data analysis was performed using SPSS 18.0 software package. RESULTS: The clinical effect of HCQ+modified Xiaoyao pill was better than that of the HCQ. The REU, VAS scores were lower after treatment in both groups (P 0.05). CONCLUSIONS: Modified Xiaoyao pill was effective and safe in the treatment of OLP patients with anxiety or depression, especially for EOLP.
{"title":"Clinical evaluation of modified Xiaoyao pill on the treatment of oral lichen planus patients with anxiety or depression","authors":"Liu Wei-dong, Zhao Min","doi":"10.19439/J.SJOS.2019.03.018","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.03.018","url":null,"abstract":"PURPOSE: To investigate the clinical effect and safety of modified Xiaoyao pill on the treatment of oral lichen planus (OLP) patients with anxiety or depression. METHODS: Sixty OLP patients with anxiety and depression were randomly divided into 2 groups, treated separately with hydroxychloroquine(HCQ, control group) and HCQ+modified Xiaoyao pill(experimental group). The results were measured with semi quantitative reticular erosive and ulcerative lesion (REU) and visual analogue scale（VAS） scoring system at the first visit, the second week, the fourth week and the eighth week, respectively. Data analysis was performed using SPSS 18.0 software package. RESULTS: The clinical effect of HCQ+modified Xiaoyao pill was better than that of the HCQ. The REU, VAS scores were lower after treatment in both groups (P 0.05). CONCLUSIONS: Modified Xiaoyao pill was effective and safe in the treatment of OLP patients with anxiety or depression, especially for EOLP.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"69 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120919318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19439/J.SJOS.2019.03.012
Yang Jang, Lin Ya-zhen, L. Ying, Zhang Qiang, Yuan Xiao
PURPOSE To investigate the prevalence of malocclusion and the correlation between caries and malocclusion in mixed dentition of primary students in Laoshan district of Qingdao city, to provide a scientific basis for early orthodontic treatment. METHODS According to WHO's oral health survey (basic methods), a survey concerning oral health was performed in 1803 children aged 8-9 years old. SPSS 17.0 software package was used for statistical analysis . RESULTS 1322 of 1803 pupils suffered from malocclusion, the prevalence of malocclusion in children surveyed was (73.32%). There were 519 cases(39.26%) of Class I malocclusion, 374 cases(28.29%) of Class II malocclusion, 357(27.00%) cases of Class III malocclusion, 72 cases (5.45%) with asymmetric malformation. As regard to clinical manifestations, deep overbite accounted for(37.67%) ,followed by deep overjet(29.19%), dental crowding (16.94%) and crossbite(16.03%). The prevalence of caries was 64.23%. Among them, there were 625 males (34.66%) and 532 females(29.51%). CONCLUSIONS The prevalence rate of malocclusion in mixed dentition of primary students in Laoshan district of Qingdao district is not optimistic, the incidence of malocclusion is closely related to caries.
{"title":"Epidemiological survey of malocclusion in 8-9 year-old children in Qingdao Laoshan district","authors":"Yang Jang, Lin Ya-zhen, L. Ying, Zhang Qiang, Yuan Xiao","doi":"10.19439/J.SJOS.2019.03.012","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.03.012","url":null,"abstract":"PURPOSE To investigate the prevalence of malocclusion and the correlation between caries and malocclusion in mixed dentition of primary students in Laoshan district of Qingdao city, to provide a scientific basis for early orthodontic treatment. METHODS According to WHO's oral health survey (basic methods), a survey concerning oral health was performed in 1803 children aged 8-9 years old. SPSS 17.0 software package was used for statistical analysis . RESULTS 1322 of 1803 pupils suffered from malocclusion, the prevalence of malocclusion in children surveyed was (73.32%). There were 519 cases(39.26%) of Class I malocclusion, 374 cases(28.29%) of Class II malocclusion, 357(27.00%) cases of Class III malocclusion, 72 cases (5.45%) with asymmetric malformation. As regard to clinical manifestations, deep overbite accounted for(37.67%) ,followed by deep overjet(29.19%), dental crowding (16.94%) and crossbite(16.03%). The prevalence of caries was 64.23%. Among them, there were 625 males (34.66%) and 532 females(29.51%). CONCLUSIONS The prevalence rate of malocclusion in mixed dentition of primary students in Laoshan district of Qingdao district is not optimistic, the incidence of malocclusion is closely related to caries.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"182 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132086527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-06-01DOI: 10.19439/J.SJOS.2019.03.007
Ji Guo-ping, Tian Yi-hong, Liu Mei-xi, Shen Ying, S. Gang
PURPOSE: This study was aimed to figure out the way that cyclic-stretch influenced the apoptosis of myoblasts and evaluate the importance of PERK and its possible mechanism involved. METHODS: L6 rat myoblasts were cultured in vitro and mechanical stimulation model was constructed successfully. The myoblasts were imposed tension for 0, 2, 6, 12 and 24 hours respectively by multi-channel cell stress loading system. The force value was 15% cell deformation and the frequency was 10 cycles/min. Each cycle was consisted of stretch for 3 seconds and relaxation for 3 seconds, and the group without tension was used as the control group. The apoptotic myoblasts were dyed by DAPI and observed through fluorescence microscopy to detect the apoptosis rate; the mRNA levels of PERK and CHOP in different groups were detected by real-time PCR and protein levels of PERK and p-PERK in different groups were detected by Western blot. PERK inhibitor was used to clear the role of PERK in apoptosis induced by cyclic-stretch and clarify the relationship between the endoplasmic reticulum stress and apoptosis induced by cyclic-stretch. SPSS 17.0 software package was used to analyze the data statistically. RESULTS: DAPI nuclear stain showed that cyclical tensile stress can induce apoptosis in vitro cultured myoblast. Apoptosis rate showed a trend of rising gradually over time, peaked at 24 h. After dealt with the inhibitor of PERK, the apoptosis rate of the 24 h group under the cyclic stretch showed no difference compared with the control. The results of real- time PCR showed that the mRNA of CHOP was increased with the extension loading time, while the mRNA of PERK showed no difference compared with the control. Western blot results showed that the protein level of p-PERK was increased with the extension of loading time, while the expression of PERK showed no difference compared with the control group. When PERK inhibitor added, the mRNA level of CHOP along with the protein expression level of p-PERK showed no significant difference compared to the control. CONCLUSIONS: PERK signaling pathway is involved in the apoptosis of myoblasts induced by cyclic stretch, and the possible mechanism may be closely related to the phosphorylation of PERK.
{"title":"The role and mechanism of endoplasmic reticulum stress key factor PREK in myoblast apoptosis under stress loading","authors":"Ji Guo-ping, Tian Yi-hong, Liu Mei-xi, Shen Ying, S. Gang","doi":"10.19439/J.SJOS.2019.03.007","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.03.007","url":null,"abstract":"PURPOSE: This study was aimed to figure out the way that cyclic-stretch influenced the apoptosis of myoblasts and evaluate the importance of PERK and its possible mechanism involved. METHODS: L6 rat myoblasts were cultured in vitro and mechanical stimulation model was constructed successfully. The myoblasts were imposed tension for 0, 2, 6, 12 and 24 hours respectively by multi-channel cell stress loading system. The force value was 15% cell deformation and the frequency was 10 cycles/min. Each cycle was consisted of stretch for 3 seconds and relaxation for 3 seconds, and the group without tension was used as the control group. The apoptotic myoblasts were dyed by DAPI and observed through fluorescence microscopy to detect the apoptosis rate; the mRNA levels of PERK and CHOP in different groups were detected by real-time PCR and protein levels of PERK and p-PERK in different groups were detected by Western blot. PERK inhibitor was used to clear the role of PERK in apoptosis induced by cyclic-stretch and clarify the relationship between the endoplasmic reticulum stress and apoptosis induced by cyclic-stretch. SPSS 17.0 software package was used to analyze the data statistically. RESULTS: DAPI nuclear stain showed that cyclical tensile stress can induce apoptosis in vitro cultured myoblast. Apoptosis rate showed a trend of rising gradually over time, peaked at 24 h. After dealt with the inhibitor of PERK, the apoptosis rate of the 24 h group under the cyclic stretch showed no difference compared with the control. The results of real- time PCR showed that the mRNA of CHOP was increased with the extension loading time, while the mRNA of PERK showed no difference compared with the control. Western blot results showed that the protein level of p-PERK was increased with the extension of loading time, while the expression of PERK showed no difference compared with the control group. When PERK inhibitor added, the mRNA level of CHOP along with the protein expression level of p-PERK showed no significant difference compared to the control. CONCLUSIONS: PERK signaling pathway is involved in the apoptosis of myoblasts induced by cyclic stretch, and the possible mechanism may be closely related to the phosphorylation of PERK.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117184891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2019-04-25DOI: 10.19439/J.SJOS.2019.02.022
Jiang Wei, Xiaoxu Song, Y. Hui, Li Xiao-zhi
PURPOSE This study was to evaluate the upper airway changes in patients with skeletal Class Ⅲ maxillary retrognathia after rapid maxillary expansion and protraction. METHODS An electronic search in PubMed, Cochrane Library, Embase, CNKI, CBM, VIP and Wanfang was performed until March 1st, 2018. According to the inclusion and exclusion criteria, two investigators respectively reviewed the literature and selected eligible studies, then assessed the risks of bias and extracted the data of the included studies. The extracted data were quantitatively analyzed with Revman 5.3 software. RESULTS A total of 12 studies were included for meta-analysis. The results showed that, after treatment the nasopharyngeal volum(P 0.05) and hypopharyneal volume (P>0.05) existed. The nasopharyngeal airway dimensions had increased significantly with the following measurements: PNS-ad1 (P 0.05), IPS(P>0.05). CONCLUSIONS Rapid maxillary expansion and protraction can increase nasopharyngeal volume and sagittal airway dimensions in skeletal Class Ⅲ subjects with maxillary retrusion. It may be suggested that rapid maxillary expansion and protraction have the potential to reduce the risk of obstructive sleep apnea syndrome in children with maxillary retrusion by enlarging airway space.
{"title":"[Upper airway changes in patients with skeletal Class III maxillary retrognathia after rapid maxillary expansion and protraction: a meta-analysis].","authors":"Jiang Wei, Xiaoxu Song, Y. Hui, Li Xiao-zhi","doi":"10.19439/J.SJOS.2019.02.022","DOIUrl":"https://doi.org/10.19439/J.SJOS.2019.02.022","url":null,"abstract":"PURPOSE This study was to evaluate the upper airway changes in patients with skeletal Class Ⅲ maxillary retrognathia after rapid maxillary expansion and protraction. METHODS An electronic search in PubMed, Cochrane Library, Embase, CNKI, CBM, VIP and Wanfang was performed until March 1st, 2018. According to the inclusion and exclusion criteria, two investigators respectively reviewed the literature and selected eligible studies, then assessed the risks of bias and extracted the data of the included studies. The extracted data were quantitatively analyzed with Revman 5.3 software. RESULTS A total of 12 studies were included for meta-analysis. The results showed that, after treatment the nasopharyngeal volum(P 0.05) and hypopharyneal volume (P>0.05) existed. The nasopharyngeal airway dimensions had increased significantly with the following measurements: PNS-ad1 (P 0.05), IPS(P>0.05). CONCLUSIONS Rapid maxillary expansion and protraction can increase nasopharyngeal volume and sagittal airway dimensions in skeletal Class Ⅲ subjects with maxillary retrusion. It may be suggested that rapid maxillary expansion and protraction have the potential to reduce the risk of obstructive sleep apnea syndrome in children with maxillary retrusion by enlarging airway space.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124646549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-25DOI: 10.19439/J.SJOS.2018.06.002
郭延伟, 杨世茂
PURPOSE To investigate the feasibility of repairing damaged cartilage of rabbit condylar with TGF-β3-transfected adipose stem cells combined with OGP (osteogenesis peptide)-HA (hyaluronic acid)-ChS (chondroitin sulfate) scaffold. METHODS Rabbit ADSCs were isolated and cultured. The expression vector carrying TGF-β3 gene was constructed using recombinant adenovirus and transfected into rabbit ADSCs. The fluorescence expression of the cells was observed after 14 days and the efficiency of virus transfection was calculated. Western blot was used to detect the expression of TGF-β3 protein. Fifty rabbits were randomly divided into 5 groups: group A as blank group, group B as TGF-β3 transfected ADSCs group, group C as OGP-HA-ChS scaffold group, group D as ADSCs complex OGP-HA-ChS group, E group was TGF-β3 transfected ADSCs with OGP-HA-ChS. After the rabbit temporomandibular joint osteoarthrosis model was established, the animals were transplanted according to the experimental design. The animals in each group were sacrificed after the 3rd and 9th weeks of transplantation. Scanning electron microscopy, histological observation and real-time fluorescent quantitative PCR were performed accordingly. SPSS17.0 software package was used for analysis of variance and t test. RESULTS Scanning electron microscopy and histological observation showed that the repair of cartilage lesions in group D and E was better than those in group B and C. Compared with group B, the results of real-time quantitative PCR showed that the expression of MMP-3 in group E was similar to that in group A, but significantly lower than that in group C and D (P<0.05). The expression of TIMP-1 in group E was similar to that of group A, but significantly higher than group C and D (P<0.05). CONCLUSIONS TGF-β3 transfected ADSCs composite OGP-HA-ChS scaffold has repairing effect on rabbit condylar damaged cartilage.
{"title":"[Effect of TGF-β3 transfected with adipose derived stem cells and OGP-HA-chondroitin sulfate scaffold on repair of condylar cartilage in rabbits].","authors":"郭延伟, 杨世茂","doi":"10.19439/J.SJOS.2018.06.002","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.002","url":null,"abstract":"PURPOSE To investigate the feasibility of repairing damaged cartilage of rabbit condylar with TGF-β3-transfected adipose stem cells combined with OGP (osteogenesis peptide)-HA (hyaluronic acid)-ChS (chondroitin sulfate) scaffold. METHODS Rabbit ADSCs were isolated and cultured. The expression vector carrying TGF-β3 gene was constructed using recombinant adenovirus and transfected into rabbit ADSCs. The fluorescence expression of the cells was observed after 14 days and the efficiency of virus transfection was calculated. Western blot was used to detect the expression of TGF-β3 protein. Fifty rabbits were randomly divided into 5 groups: group A as blank group, group B as TGF-β3 transfected ADSCs group, group C as OGP-HA-ChS scaffold group, group D as ADSCs complex OGP-HA-ChS group, E group was TGF-β3 transfected ADSCs with OGP-HA-ChS. After the rabbit temporomandibular joint osteoarthrosis model was established, the animals were transplanted according to the experimental design. The animals in each group were sacrificed after the 3rd and 9th weeks of transplantation. Scanning electron microscopy, histological observation and real-time fluorescent quantitative PCR were performed accordingly. SPSS17.0 software package was used for analysis of variance and t test. RESULTS Scanning electron microscopy and histological observation showed that the repair of cartilage lesions in group D and E was better than those in group B and C. Compared with group B, the results of real-time quantitative PCR showed that the expression of MMP-3 in group E was similar to that in group A, but significantly lower than that in group C and D (P<0.05). The expression of TIMP-1 in group E was similar to that of group A, but significantly higher than group C and D (P<0.05). CONCLUSIONS TGF-β3 transfected ADSCs composite OGP-HA-ChS scaffold has repairing effect on rabbit condylar damaged cartilage.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"216 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115068591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-01DOI: 10.19439/J.SJOS.2018.06.005
Wang Yinghui, Qiu Fen-fang, P. Yu, Wu Wei-qing, Cheng Hui
PURPOSE To investigate the effects of recasting on metallographic microstructure of cobalt-chromium(Co-Cr), commercially pure titanium(cpTi), palladium-copper-gallium(Pd-Cu-Ga), aurum-platinum(Au-Pt) ceramic alloys. METHODS Without adding new alloys, Co-Cr, cpTi, Pd-Cu-Ga and Au-Pt ceramic alloys were recasted for 1-3 times under the circumstance of vacuum compressive casting with argon. Before each recasting, these 4 ceramic alloys casted previously were treated by the most appropriate way which has been reported by our previous study. According to GB/T13298-2015 standards, the specimens of 4 ceramic alloys after recasting 1-3 times were grinded, polished, and etched and then the metallographic microstructure of them were determined by a metallographic microscope or scanning electron microscopy(SEM). RESULTS With the increase of recasting times, the microstructure of Co-Cr ceramic alloy showed grain growth, grain matrix increase, and the intermetallic compound in the grain boundary increase, the microstructure of the cpTi ceramic alloy showed grain growth. The samples of cpTi ceramic alloy recasting twice appeared shaft tendency, and the ones which were recasted 3 times were compound of α-phase and β-phase. The microstructure of the Pd-Cu-Ga ceramic alloy showed grain growth slightly after casting 2-3 times, while no significant change was found on the microstructure of the Au-Pt ceramic alloy. CONCLUSIONS Recasting changes the microstructure of Co-Cr, cpTi, and Pd-Cu-Ga ceramic alloys, and the ceramic alloys may recycled by the manufacturers.
{"title":"The effect of recasting on microstructure of dental ceramic alloys","authors":"Wang Yinghui, Qiu Fen-fang, P. Yu, Wu Wei-qing, Cheng Hui","doi":"10.19439/J.SJOS.2018.06.005","DOIUrl":"https://doi.org/10.19439/J.SJOS.2018.06.005","url":null,"abstract":"PURPOSE To investigate the effects of recasting on metallographic microstructure of cobalt-chromium(Co-Cr), commercially pure titanium(cpTi), palladium-copper-gallium(Pd-Cu-Ga), aurum-platinum(Au-Pt) ceramic alloys. METHODS Without adding new alloys, Co-Cr, cpTi, Pd-Cu-Ga and Au-Pt ceramic alloys were recasted for 1-3 times under the circumstance of vacuum compressive casting with argon. Before each recasting, these 4 ceramic alloys casted previously were treated by the most appropriate way which has been reported by our previous study. According to GB/T13298-2015 standards, the specimens of 4 ceramic alloys after recasting 1-3 times were grinded, polished, and etched and then the metallographic microstructure of them were determined by a metallographic microscope or scanning electron microscopy(SEM). RESULTS With the increase of recasting times, the microstructure of Co-Cr ceramic alloy showed grain growth, grain matrix increase, and the intermetallic compound in the grain boundary increase, the microstructure of the cpTi ceramic alloy showed grain growth. The samples of cpTi ceramic alloy recasting twice appeared shaft tendency, and the ones which were recasted 3 times were compound of α-phase and β-phase. The microstructure of the Pd-Cu-Ga ceramic alloy showed grain growth slightly after casting 2-3 times, while no significant change was found on the microstructure of the Au-Pt ceramic alloy. CONCLUSIONS Recasting changes the microstructure of Co-Cr, cpTi, and Pd-Cu-Ga ceramic alloys, and the ceramic alloys may recycled by the manufacturers.","PeriodicalId":436266,"journal":{"name":"Chinese Journal of Oral Implantology","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131200861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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