Relatedness of Strains ΔH and Marburg of Methanobacterium thermoautotrophicum

Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie Pub Date : 1981-12-01 DOI:10.1016/S0721-9571(81)80023-3

Astrid Brandis , Rudolf K. Thauer , Karl O. Stetter

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引用次数: 66

Abstract

Methanobacterium thermoautotrophicum ΔH and M. thermoautotrophicum Marburg have been investigated for DNA relatedness by DNA-DNA hybridization. The two strains proved to be interrelated on the 46% binding level. The relatively low degree of DNA homology correlated with differences in biochemical and physiological characteristics:

1.
The pseudomurein sacculus of the ΔH strain contained glucosamine and talosaminuronic acid as major sugar components, whereas the sacculus of the Marburg strain contained galactosamine and talosominuronic acid.
2.
One of the eight subunits of the DNA dependent RNA polymerase differed considerably with respect to molecular weight.
3.
The membrane fraction of the ΔH strain exhibited high ATPase activity, which could not be detected in the Marburg strain.
4.
The Marburg strain grew more rapidly (td = 1.6 h versus 2.2 h) and to a higher cell density than the ΔH strain.

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热自养甲烷杆菌ΔH与马尔堡菌的亲缘关系

通过DNA-DNA杂交研究了热自养甲烷杆菌ΔH和马尔堡热自养杆菌的DNA亲缘性。结果表明，这两个菌株在46%的结合水平上相互关联。DNA同源度较低与生物化学生理特性差异相关:1。ΔH菌株的假尿蛋白囊以葡萄糖胺和塔洛糖氨基醛酸为主要糖成分，而马尔堡菌株的囊以半乳糖胺和塔洛糖氨基醛酸为主要糖成分。依赖DNA的RNA聚合酶的八个亚基中有一个在分子量方面差别很大。ΔH菌株的膜组分表现出较高的atp酶活性，这在马尔堡菌株中没有检测到。与ΔH菌株相比，马尔堡菌株生长速度更快(td = 1.6 h比2.2 h)，细胞密度更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Zentralblatt für Bakteriologie Mikrobiologie und Hygiene: I. Abt. Originale C: Allgemeine, angewandte und ?kologische Mikrobiologie

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