H. Itoh, N. Makimura, T. Arai, H. Tsubamoto, K. Furuya, M. Kikuchi, I. Nagata
{"title":"Influence of light irradiation an the development of mouse eggs in culture","authors":"H. Itoh, N. Makimura, T. Arai, H. Tsubamoto, K. Furuya, M. Kikuchi, I. Nagata","doi":"10.1109/IEMBS.1993.979316","DOIUrl":null,"url":null,"abstract":"We tnvestlgated the Influence of l lght envlronment on the development of mouse egg in virro. The 1-cell mouse eggs were irradlated by varlous laser l ights of whlch wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual llght environment by means of the microscope IIght source In IVFET ( in vitro fertlllzatlon-embryo transfer) was measured to set the I lght dose In th is experlment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500kJ/m2 I n dose ( 2 0 t lmes larger than actual l lght envlronment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed In egg division. These results suggested that l lght environment migh t af fect o n the development of mouse egg in vitro.","PeriodicalId":408657,"journal":{"name":"Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ","volume":"32 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"1993-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 15th Annual International Conference of the IEEE Engineering in Medicine and Biology Societ","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/IEMBS.1993.979316","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
We tnvestlgated the Influence of l lght envlronment on the development of mouse egg in virro. The 1-cell mouse eggs were irradlated by varlous laser l ights of whlch wavelengths were 632.8nm, 543nm, and 442nm in vitro. Actual llght environment by means of the microscope IIght source In IVFET ( in vitro fertlllzatlon-embryo transfer) was measured to set the I lght dose In th is experlment. Mouse eggs were cultured to observe cellular development stage. In case of 632.8nm radiation of 500kJ/m2 I n dose ( 2 0 t lmes larger than actual l lght envlronment), 97% of eggs stopped their division. In the case of 543nm and 442nm radiations no influences were observed In egg division. These results suggested that l lght environment migh t af fect o n the development of mouse egg in vitro.
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