Genomics and molecular physiology for improvement of drought tolerance in wheat.

S. Sareen, P. Saini, Charan Singh, P. Kumar, S. Sheoran
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Abstract

Abstract This chapter discusses the complexity of drought tolerance in wheat focusing the morphological, biochemical, physiological and molecular responses. The breeding approaches, such as traditional and genomics-assisted strategies, for drought tolerance in wheat are described. Future perspectives are also mentioned. Before wheat genome sequencing, it was very difficult to dissect drought tolerance genomic regions because of large genome size and repetitive sequences. But with the availability of sequencing approaches, a large number of genomic resources has become available which extend the scope of utilization of advanced genomics approaches such as GWAM and GS, MutMap+, etc. A new genome editing approach, the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPRassociated protein 9 (Cas9) system, can also be utilized for enhancement of drought tolerance in wheat. Therefore, integration of genomic approaches with precise phenotyping is the need of the hour for improving drought tolerance in wheat.
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小麦耐旱性改良的基因组学和分子生理学研究。
摘要本章从形态、生化、生理和分子等方面探讨了小麦抗旱性的复杂性。介绍了小麦抗旱性的传统育种方法和基因组学辅助育种方法。未来的展望也被提及。在小麦基因组测序之前,由于小麦基因组的大尺寸和重复序列,对耐旱基因组区域进行解剖是非常困难的。但随着测序方法的可用性,大量基因组资源的可用性扩大了先进基因组学方法的使用范围,如GWAM和GS、MutMap+等。一种新的基因组编辑方法,即聚集规律间隔短回文重复序列(CRISPR)/CRISPR相关蛋白9 (Cas9)系统,也可用于增强小麦的耐旱性。因此,整合基因组方法和精确表型是提高小麦耐旱性的迫切需要。
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The genetic architecture and breeding towards cold tolerance in maize: review. Molecular breeding for increasing micronutrient content in sorghum. CRISPR-mediated gene editing in wheat for abiotic stress tolerance. Recent advancement of molecular breeding for improving salinity tolerance in wheat. Recent understanding on molecular mechanisms of plant abiotic stress response and tolerance.
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