OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.)

IF 6 1区农林科学 Q1 AGRONOMY Crop Journal Pub Date : 2023-12-01 DOI:10.1016/j.cj.2023.05.012

Qiaoling Liao , Xinle Cheng , Tong Lan , Xiaokuan Guo , Zilong Su , Xiaoxiao An , Yali Zheng , Haitao Cui , Weiren Wu , Tao Lan

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Abstract

Plant trichomes are a specialized cellular tissue that functions in resistance to biotic and abiotic stresses. In rice, three transcription-factor genes: OsWOX3B, HL6, and OsSPL10, have been found to control trichome development. Although studies have shown interactions between the three genes, their full relationship in trichome development is unclear. We found that the expression levels of OsWOX3B and HL6 were both reduced in OsSPL10-knockout plants but increased in OsSPL10-overexpression plants, suggesting that OsSPL10 positively regulates their expression. Physical interaction between OsSPL10 and OsWOX3B was found both in vivo and in vitro and attenuated their abilities to bind to the promoter of HL6 to activate its transcription. This mechanism may regulate trichome length by adjusting the expression of HL6. A rice gene network regulating trichome development is proposed.

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OsSPL10 通过与 OsWOX3B 在转录和蛋白质水平上的相互作用控制水稻（Oryza sativa L. ）毛状体的发育

植物毛状体是一种特殊的细胞组织，具有抵抗生物和非生物胁迫的功能。在水稻中，有三个转录因子基因：已发现 OsWOX3B、HL6 和 OsSPL10 这三个转录因子基因控制毛状体的发育。尽管研究表明这三个基因之间存在相互作用，但它们在毛状体发育过程中的全部关系尚不清楚。我们发现，OsWOX3B和HL6的表达水平在OsSPL10基因敲除植株中都有所降低，但在OsSPL10基因高表达植株中却有所提高，这表明OsSPL10对它们的表达有正向调节作用。OsSPL10 和 OsWOX3B 在体内和体外都发生了物理相互作用，削弱了它们与 HL6 启动子结合以激活其转录的能力。这一机制可能是通过调节 HL6 的表达来调节毛状体的长度。提出了一个调控毛状体发育的水稻基因网络。

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来源期刊

Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

9.90

自引率

3.00%

发文量

638

审稿时长

41 days

期刊介绍： The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.