Evaluation of Effect on Combination of Five Medicinal Plants Extracts on Denaturation of Protein - A Remedy of Arthritis Disease

Abstract

Arthritis is a type of autoimmune disorder and a major cause of disability throughout the world. Rheumatoid arthritis the denaturation of protein is one of the causes. In our present study, methanolic extract of combination of plant (Tinospora cordifolia (Thunb.) Miers, Curcuma longa L., Pongamia pinnata (L.) Panigrahi, Emblica officinalis L., Piper nigrum L.). Extracts inhibited heat induced protein denaturation and may be one of the reasons of possessing anti-arthritic activity. Methanolic extracts of different formulation for their antiarthritic activity against denaturation of bovine serum albumin. Result and attributed the 15 formulation out of F2 formulation possessed highly denaturation of protein. The denaturation of protein F2formulation presence of active principle such as alkaloids, flavonoids, saponins and terpenoids and HPTLC analysis major 2 bioactive compounds present which are responsible for this activity. GC-MS analysis of methanol extracts identified major bioactive with peak area 36.92 % is maximum in F2 formulation (10mg/10ml). In fact, herbal plants formulation of five plants for the F2 formulation are being used widely as medicine around decades for treatment of arthritic disease. Herbal medicine constitutes important resources for the treatment of arthritis disease.