Enzymatic Treatment of Enhanced Musa Peel Flour as Potential Low Digestible Starch

Nor Hasmaliana Abdul Manas, Khairun Mahfuzah Khairil Mokhtar, Siti Fatimah Zaharah Mohd Fuzi, R. Che Man, A. C. Khayrani
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Abstract

Enhanced musa peel flour has a great potential as a low digestible starch that is beneficial for diabetic patients. Low digestible starch prevents the rapid increase of blood-glucose level which is crucial to protect from internal organ from damage. However, the black colour of the flour which is due to the phenolic compound oxidation in the cell wall has affected the sensory evaluation of consumers. In this work, instead of using conventional chemical treatment, enzymatic treatment is utilized to reduce the dark colour of the flour. Cell wall disruption by degradation of hemicellulose component by xylanase was hypothesized to accelerate the removal of the dark pigment. Colour reduction was optimized by varying xylanase concentration and incubation duration. Next, starch digestibility analysis was conducted using amylase enzyme to determine the starch digestibility fractions in the treated flour. As a result, instead of obtaining significant colour reduction of the flour, it is observed that there is a significant increment of resistant starch content (14%) in the treated enhanced musa peel flour. Meanwhile, reduction of 86.44% of rapidly digested starch content in the treated flour was also obtained. As a conclusion, the disruption of the cell wall by xylanase lead to the increase of the resistant starch content in the enhanced musa peel flour.
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增强木薯皮粉作为潜在低消化淀粉的酶处理
增强的musa皮面粉作为一种低消化淀粉具有很大的潜力,对糖尿病患者有益。低可消化淀粉阻止血糖水平的快速上升,而血糖水平对保护内脏器官免受损害至关重要。然而,由于细胞壁中酚类化合物氧化导致的面粉的黑色影响了消费者的感官评价。在这项工作中,使用酶处理代替传统的化学处理来减少面粉的深色。据推测,木聚糖酶降解半纤维素组分破坏细胞壁可以加速深色色素的去除。不同浓度的木聚糖酶和不同的培养时间对还原效果最优。然后,利用淀粉酶对处理后的面粉进行淀粉消化率分析,测定淀粉消化率分数。结果,在处理过的强化木薯皮面粉中,抗性淀粉含量显著增加(14%),而不是显著降低面粉的颜色。同时,处理后的面粉中快速消化淀粉含量降低了86.44%。结果表明,木聚糖酶对细胞壁的破坏导致了增强后木薯皮粉中抗性淀粉含量的增加。
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