{"title":"Random amplified polymorphic DNA -RAPD analysis of human cervical\ncancer in Samwha city","authors":"Mohammed Abdulwahab Ati Al-Askeri","doi":"10.21931/rb/css/s2023.08.01.9","DOIUrl":null,"url":null,"abstract":"Objectives: The study intended to distinguish a likely hereditary variety in the\ngenomic DNA of Iraqi patients corresponding with cervical disease and the possibility of getting an advantage of the potentially produced DNA fragment(s) as a\nmolecular test for future related applications. Methods: Blood and tissue tests\nwere collected from female patients with cervical malignant growth and solid\nfemales; DNA was extricated and purged from each example, and then \"Irregular\nAmplification of Polymorphic DNA technique\"(RAPD) was directed with oligonucleotide \"OPA-20\". Results: RAPD results examination with oligonucleotide\n“OPA-20” could identify four polymorphic DNA groups in the genome of patients females, in which these bands were missing in the majority of genomic\nDNA of healthy people.\nKeywords: Tumor, PCR, Iraq, gene polymorphism","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"22 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sumer 1","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21931/rb/css/s2023.08.01.9","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objectives: The study intended to distinguish a likely hereditary variety in the
genomic DNA of Iraqi patients corresponding with cervical disease and the possibility of getting an advantage of the potentially produced DNA fragment(s) as a
molecular test for future related applications. Methods: Blood and tissue tests
were collected from female patients with cervical malignant growth and solid
females; DNA was extricated and purged from each example, and then "Irregular
Amplification of Polymorphic DNA technique"(RAPD) was directed with oligonucleotide "OPA-20". Results: RAPD results examination with oligonucleotide
“OPA-20” could identify four polymorphic DNA groups in the genome of patients females, in which these bands were missing in the majority of genomic
DNA of healthy people.
Keywords: Tumor, PCR, Iraq, gene polymorphism
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