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Synergistic Treatment of Candida albicans Infected Mice by AgNPsSynthesis Penicillium chrysogenum agnps合成青霉菌协同治疗白色念珠菌感染小鼠
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.67
Noor Naeem Jasim, Rasha M. A. Al-humairi, T. Hashim Mohammad, Ruwaida A. Hussein
Penicillium chrysogenum has been used for the production of metalnanoparticles. It was experimentally shown that silver nitrate ions are reduced tosilver nanoparticles in biomass, and silver nanoparticles are synthesized from 1mM silver nitrate. The external morphology of ground AgNPs was established bySEM analysis and UV-visible spectrophotometry, and the size of AgNO3 particles was observed to be 23 nm, with particles being spherical by AFM. In vitroinvestigations, the antifungal susceptibility of Candida al was calculated, and theresults showed that the inhibitory effect of AgNPs synthesized with P. chrysogenum is increased with increasing the concentration of nanoparticles, and the effective fungicidal concentration (EC50) was 1 ppm, and the minimum fungicidalconcentration (MFC) was 100 ppm. Furthermore, the pathogenicity of C. albicans on kidneys in mice during infections was tested and thus indicated that thekidneys' ability was significantly enhanced when animals were treated with thecombination of AgNPs and P. chrysogenum. This study's data from examinationprovide valuable preliminary statistics for using biosynthesized silver nanoparticles to manage various microorganisms.Keywords: Candida albicans, AgNPs Synthesis, Penicillium chrysogenum
青霉菌已被用于生产金属纳米颗粒。实验表明,硝酸银离子在生物质中被还原为纳米银,并以1mM硝酸银为原料合成纳米银。通过sem分析和紫外可见分光光度法确定了AgNPs的外部形貌,通过AFM观察到AgNO3颗粒的尺寸为23 nm,颗粒呈球形。体外研究中,计算了假丝酵母的抗真菌敏感性,结果表明,用P. chrysogenum合成的AgNPs的抑制效果随着纳米颗粒浓度的增加而增强,其有效杀真菌浓度(EC50)为1 ppm,最低杀真菌浓度(MFC)为100 ppm。此外,我们还测试了感染期间白色念珠菌对小鼠肾脏的致病性,从而表明,当动物联合使用AgNPs和P. chrysogenum时,肾脏的能力显着增强。本研究的检测数据为使用生物合成银纳米颗粒管理各种微生物提供了有价值的初步统计数据。关键词:白色念珠菌,AgNPs合成,青霉菌
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引用次数: 0
Subchronic intraperitoneal toxicity of Sio2NPs on body weight andthyroid gland hormones in female Rats Sio2NPs对雌性大鼠体重和甲状腺激素的亚慢性腹腔毒性
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.59
Riam Sabah Abbood, Noori M. Luaibi
This study investigated the toxicity effect of Sio2NPs on body weightand thyroid gland in female rats; the experiment was included. The experimentalanimals were randomly divided into three groups, including two treatment groups( treated with a low dose (25mg/Kg ) and high dose (100mg/Kg of body weight)of sio2 NPs and one group as a control. Each group of them was divided intothree subgroups according to the injection periods of exposure (10, 20, and 30)days daily, then measure the body weight and Thyroid function hormones (T3,T4, TSH), the results showed that there was a significant decrease (p<0.05) in thebody weight of animals that exposed to SiO2 NPs at two doses (25 and 100mg/kg) during (10 days) while increased during (20, 30 days) but the highest value recorded in the animals that were treated for (30 days) at both doses. On theother side, the T3 findings recorded a significant decrease (p< 0.05) for the animals that exposed to SiO2 NPs at low and high doses (25 and 100 mg/kg) (during(10 days) when compared with the control group As well as, there was a significant decrease (p< 0.05) in the level of T3 for all animals that treated with thesedoses during (20 and 3 days) in serum of animals that were treated with both doses of SiO2 NPs when compared with the control group (p< 0.05), while T4showed s decreasing in the treated group during (10 and 20, 30 days) comparedto a control group with highly significant ((p< 0.05), finally while TSH showed ahighly increasing in treated group during (10 and 20, 30 days) compared to control group (p< 0.05). The study revealed that Exposure to Sio2NPs at differentconcentrations caused structural and functional and in the thyroid gland, characterized decrease in T3 and T4 hormone levels and an increase in the TSH hormone level and caused hypothyroidism This led to a decrease in the metabolismprocess and thus the body weight of animals gained a significant increase.Keywords: nanoparticles, Sio2NPs, Thyroid gland, T3, T4, TSH, Body weight,female rats.
研究了Sio2NPs对雌性大鼠体重和甲状腺的毒性作用;实验被纳入。实验动物随机分为3组,分别给予低剂量(25mg/Kg)和高剂量(100mg/Kg体重)sio2 NPs处理组,另设对照组。每组的划分intothree子组根据注射时间的暴露(10、20和30天,然后测量体重和甲状腺功能激素(T3、T4、TSH),结果表明,有一个人体体重显著降低(p < 0.05)的动物暴露于二氧化硅在两剂NPs(25和100毫克/公斤)在(10天),同时增加在(20、30天),但最高的值记录的动物治疗剂量(30天)。在另一边,T3发现记录明显降低(p < 0.05)的动物暴露于二氧化硅NPs在低和高剂量(25和100毫克/公斤)(期间(10天)同对照组相比,有显著降低(p < 0.05)水平的T3为所有动物对待thesedoses(20 - 3天)中动物的血清,治疗剂量的二氧化硅NPs与对照组相比(p < 0.05),治疗组TSH(10、20,30 d)显著高于对照组(p< 0.05),治疗组TSH(10、20,30 d)显著高于对照组(p< 0.05)。研究发现,暴露于不同浓度的Sio2NPs会引起甲状腺结构和功能的改变,表现为T3和T4激素水平降低,TSH激素水平升高,引起甲状腺功能减退,导致代谢过程减少,从而使动物体重显著增加。关键词:纳米粒子,sio2纳米粒子,甲状腺,T3, T4, TSH,体重,雌性大鼠
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引用次数: 0
Prevalence of parallel infection of dental caries and tonsillitis amongsome Iraqi children 一些伊拉克儿童龋齿和扁桃体炎并行感染的流行
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.84
Dunia B. Shabeeb, Eman N. Naji
Aims and Objectives: This study aims to investigate the role of dentalcaries-causing bacteria in increasing acute or chronic tonsillitis among Iraqi children. Materials and Methods: 120 patients were part of the study and were divided into 6 groups; each group had 20 patients aged 6–12. Swab samples were obtained from dental plaque and tonsils to identify the bacterial flora. Identificationmethods included metabolic capability, microscopic reading, and biochemical reaction. Results: The results showed that Streptococcus pneumonia was the mostcommon Gram-positive bacterial species isolated from three different groups andcontrol rate (32.9 %). Also, Moraxella catarrhalis, Granulicatella elegant andStreptococcus parasanguinis appeared in both tonsillitis and dental caries. Conclusion: An interesting association between dental caries and tonsillitis in children aged 6 to 12 appeared. Most Gram-positive and Gram-negative bacterial isolates were multi-drug resistant to antimicrobial agents.Keywords: parallel infection, dental caries, tonsillitis, Iraqi children.
目的和目的:本研究旨在调查引起龋齿的细菌在伊拉克儿童急性或慢性扁桃体炎中所起的作用。材料与方法:120例患者分为6组;每组患者20例,年龄6 ~ 12岁。从牙菌斑和扁桃体中提取棉签样本以确定细菌菌群。鉴定方法包括代谢能力、显微读数和生化反应。结果:三组分离的革兰氏阳性菌中以肺炎链球菌最为常见,控制率为32.9%。扁桃体炎和龋齿均可见卡他莫拉菌、细粒菌和副鳗链球菌。结论:6 ~ 12岁儿童龋齿与扁桃体炎之间存在有趣的联系。大多数革兰氏阳性和革兰氏阴性分离菌对抗菌药物具有多重耐药。关键词:平行感染,龋齿,扁桃体炎,伊拉克儿童。
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引用次数: 0
Effect of adding different cinnamon oil concentrations on thechemical, physical, and microbial properties of local goose liver meatduring different storage periods 添加不同肉桂油浓度对不同贮藏期本地鹅肝肉化学、物理和微生物特性的影响
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.76
Adnan Shakor Ahmed Al-Perkhdri, Sami Ibrahim Abdullah, Ahmed Sami Shaker
Cinnamon oil is a plant extract used to exert antimicrobial actionsagainst essential pathogens. 96 samples of goose liver were used and divided forfour treatments (control, T1=0.025, T2=0.050, T3=0.075) for three different storage times (1, 15, 20 days). After the storage periods, finished samples were analyzed, including chemical traits (carbohydrate, protein, ash, and moisture) andphysical traits (pH, water holding capacity, loss during cooking, and loss duringthrowing). Also, a microbial trait was measured (cold bacteria and total bacteria).Significant differences were found just in (pH, loss during throwing, cold bacteria, and total bacteria (0.000, 0.046, 0.000, and 0.000), respectively, in the threedifferent storage periods. There were statistically significant differences betweenthe different levels of cinnamon oil and the storage periods, which affected themicrobial number. Further studies are needed to test other goose meat parts withdifferent cinnamon levels.Keywords: Liver, meat, storage, chemical, physical, microbiological
肉桂油是一种植物提取物,用于对必需病原体发挥抗菌作用。选取96份鹅肝样品,分为4个处理(对照组,T1=0.025, T2=0.050, T3=0.075),分别保存1、15、20 d。贮存期结束后,对成品样品进行分析,包括化学性状(碳水化合物、蛋白质、灰分和水分)和物理性状(pH值、保水能力、烹饪过程中的损失和投掷过程中的损失)。此外,还测量了微生物特性(冷细菌和总细菌)。结果表明,3种不同贮藏期的样品只有pH值、抛粪损失、冷菌数和总菌数(0.000、0.046、0.000和0.000)存在显著差异。不同肉桂油浓度和不同贮藏期对微生物数量的影响有统计学意义。需要进一步的研究来测试其他含有不同肉桂含量的鹅肉部位。关键词:肝脏,肉类,贮藏,化学,物理,微生物
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引用次数: 0
Histological Detection and Anti-oxidant Effect of Bee Venom on thePancreas of Diabetic Mice 蜂毒对糖尿病小鼠胰腺的组织学检测及抗氧化作用
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/s2023.08.01.45
R. A. Al-Sarray, S. Al-Shaeli
Bee venom BV draws attention in the medical field to manage several diseases,including type 2 diabetes mellitus (T2DM). Due to limited research on this field inIraq, the study aimed to detect BV's histological and anti-oxidant impact in diabeticmice. Fifteen male mice were randomly assigned into three groups. The first groupis control (C), the second is diabetic, and the last group is BV. At the end of 30days, the obtained serum was used to measure the level of glucose, insulin, superoxide dismutase (SOD), and malondialdehyde (MDA). Furthermore, the pancreaswas histologically assessed using the Gomori stain. Alloxan showed significantelevation and reduction in the level of glucose and insulin compared to the control.These alterations were positively enhanced by BV. BV promoted significant elevation in the activity of SOD and suppressed MDA compared to diabetic mice.Histologically, diabetic mice pancreas displayed a reduction of capsular tissue, islets and beta cells vacuolization, beta cells initial degeneration, reduction in theshape and size of the islets and beta cells, and vascular hemorrhage. These changeswere remarkably enhanced nearly to normal by BV. Therefore, BV could controlthe diabetic condition, which may be considered a potential anti-diabetic agent.Keywords: Diabetes mellitus, SOD, MDA, pancreas, mice, BV
蜂毒BV在治疗包括2型糖尿病(T2DM)在内的几种疾病方面引起了医学界的关注。由于伊拉克在这一领域的研究有限,本研究旨在检测BV对糖尿病小鼠的组织学和抗氧化作用。15只雄性小鼠被随机分为三组。第一组为对照组(C),第二组为糖尿病患者,最后一组为BV。30d后,测定血清中葡萄糖、胰岛素、超氧化物歧化酶(SOD)、丙二醛(MDA)水平。此外,使用Gomori染色对胰腺进行组织学评估。与对照组相比,四氧嘧啶组血糖和胰岛素水平显著升高和降低。BV对这些改变有积极的促进作用。与糖尿病小鼠相比,BV可显著提高SOD活性,抑制MDA活性。组织学上,糖尿病小鼠胰腺表现为囊组织减少,胰岛和β细胞空泡化,β细胞初始变性,胰岛和β细胞形状和大小减小,血管出血。BV使这些变化明显增强,接近正常水平。因此,BV具有控制糖尿病病情的作用,可能被认为是一种潜在的降糖药。关键词:糖尿病,SOD, MDA,胰腺,小鼠,BV
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引用次数: 0
Sweet and Salt Threshold Detection among Dental Implant Patientsin Relation to Cognition Status and Salivary Acetylcholinesterase 种植牙患者糖、盐阈值检测与认知状态及唾液乙酰胆碱酯酶的关系
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.80
Rihab Abdul Hussein Ali, Ban Sahib Diab
Background: Wearing a dental implant may affect gustatory function,an essential element of health in older adults that can affect cognition status. Thisstudy aimed to assess the sweet and salt taste threshold detection among dentalimplant patients in relation to cognition status and salivary Acetylcholinesterasecompared with partially edentulous and fully dentate patients matching in ageand gender. Materials and methods: The study comprised 90 dental implant patients between the ages of 50 and 60 compared with 90 partially edentulous and90 fully dentate individuals who were age and gender-matched. The participants'cognition status was tested using the Self-Administered Gero-cognitive Examination (SAGE) test, which classified them into poor and good. A two-alternativeforced-choice question was used to establish taste threshold sensitivity, with concentrations presented in ascending order. One chemical was investigated for eachtaste quality (salt and sweet), and each component was delivered at five differentconcentrations. The enzyme-linked immunosorbent test (ELISA) kit measuredsalivary Acetylcholinesterase. Spss 22 Chicago Illionis USA was used to examine the data. Results: There was a significant association between sweet and salttaste thresholds and the patient's groups, as the higher patient occurrence concerning the lowest taste threshold was found for the dentate patients.In comparison, the higher patient occurrence for the highest taste threshold wasfound for the partially edentulous patients. Data analysis showed a significant association between cognitive status and sweet and salt taste threshold for the implant patient group, as the percentage of patients with the lowest taste thresholdwas higher for good cognitive status; the same result was found concerning partially edentulous and fully dentate patients; however, the association was not significant. The results revealed that the mean value of salivary Acetylcholinesterase(AChE) was higher among the fully dentate group, followed by implant. Then,the partially edentulous groups with statistically insignificant differences for bothpoor and good cognitive status groups. On the other hand, when comparing thepoor and good cognitive status groups, results revealed that the mean value ofsalivary Acetylcholinesterase was higher among the good than the poor groups,with statistically significant differences among all sample groups. Conclusion:Within the limitation of this investigation, it was confirmed that taste thresholddetection is connected with missing teeth and cognition status. The findings showa complicated interplay of several factors that affect gustatory acuity, includingoral health and cognition status.Keywords: Threshold, Dental Implant Patients, Salivary Acetylcholinesterase
背景:佩戴种植牙可能会影响味觉功能,而味觉功能是老年人健康的一个基本要素,可以影响认知状态。本研究旨在评估种植牙患者的甜味和盐味阈值检测与认知状态和唾液乙酰胆碱酯含量的关系,并与年龄和性别匹配的部分无牙和完全有牙患者进行比较。材料和方法:该研究包括90名年龄在50到60岁之间的种植牙患者,与90名年龄和性别匹配的部分无牙和90名完全有牙的个体进行比较。采用自我管理的老年认知测验(SAGE)对参与者的认知状况进行测试,将他们分为差和好。一个双选项强迫选择问题被用来建立味觉阈值敏感性,浓度按升序呈现。研究人员研究了一种化学物质的每种味道(盐和甜),每种成分以五种不同的浓度提供。酶联免疫吸附试验(ELISA)试剂盒测定唾液乙酰胆碱酯酶。使用Spss 22 Chicago Illionis USA对数据进行检验。结果:甜味和咸味阈值与患者群体之间存在显著相关性,有齿患者的最低味觉阈值发生率较高。相比之下,部分无牙患者的最高味觉阈值发生率更高。数据分析显示,认知状态与种植患者组的甜盐味觉阈值之间存在显著关联,因为认知状态良好的患者中,味觉阈值最低的患者比例更高;在部分无牙和全牙患者中发现了相同的结果;然而,这种关联并不显著。结果显示,全齿组唾液乙酰胆碱酯酶(AChE)均值较高,种植体次之;然后,部分缺牙组与认知状况较差和良好组的差异在统计学上不显著。另一方面,当比较认知状态差组和认知状态好的组时,结果显示,良好组的唾液乙酰胆碱酯酶均值高于认知状态差组,各样本组间差异有统计学意义。结论:在本研究的范围内,味觉阈值检测与缺牙和认知状态有关。研究结果表明,影响味觉敏锐度的几个因素之间存在复杂的相互作用,包括口腔健康和认知状态。关键词:阈值,种植牙患者,唾液乙酰胆碱酯酶
{"title":"Sweet and Salt Threshold Detection among Dental Implant Patients\u0000in Relation to Cognition Status and Salivary Acetylcholinesterase","authors":"Rihab Abdul Hussein Ali, Ban Sahib Diab","doi":"10.21931/rb/css/2023.08.01.80","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.80","url":null,"abstract":"Background: Wearing a dental implant may affect gustatory function,\u0000an essential element of health in older adults that can affect cognition status. This\u0000study aimed to assess the sweet and salt taste threshold detection among dental\u0000implant patients in relation to cognition status and salivary Acetylcholinesterase\u0000compared with partially edentulous and fully dentate patients matching in age\u0000and gender. Materials and methods: The study comprised 90 dental implant patients between the ages of 50 and 60 compared with 90 partially edentulous and\u000090 fully dentate individuals who were age and gender-matched. The participants'\u0000cognition status was tested using the Self-Administered Gero-cognitive Examination (SAGE) test, which classified them into poor and good. A two-alternative\u0000forced-choice question was used to establish taste threshold sensitivity, with concentrations presented in ascending order. One chemical was investigated for each\u0000taste quality (salt and sweet), and each component was delivered at five different\u0000concentrations. The enzyme-linked immunosorbent test (ELISA) kit measured\u0000salivary Acetylcholinesterase. Spss 22 Chicago Illionis USA was used to examine the data. Results: There was a significant association between sweet and salt\u0000taste thresholds and the patient's groups, as the higher patient occurrence concerning the lowest taste threshold was found for the dentate patients.\u0000In comparison, the higher patient occurrence for the highest taste threshold was\u0000found for the partially edentulous patients. Data analysis showed a significant association between cognitive status and sweet and salt taste threshold for the implant patient group, as the percentage of patients with the lowest taste threshold\u0000was higher for good cognitive status; the same result was found concerning partially edentulous and fully dentate patients; however, the association was not significant. The results revealed that the mean value of salivary Acetylcholinesterase\u0000(AChE) was higher among the fully dentate group, followed by implant. Then,\u0000the partially edentulous groups with statistically insignificant differences for both\u0000poor and good cognitive status groups. On the other hand, when comparing the\u0000poor and good cognitive status groups, results revealed that the mean value of\u0000salivary Acetylcholinesterase was higher among the good than the poor groups,\u0000with statistically significant differences among all sample groups. Conclusion:\u0000Within the limitation of this investigation, it was confirmed that taste threshold\u0000detection is connected with missing teeth and cognition status. The findings show\u0000a complicated interplay of several factors that affect gustatory acuity, including\u0000oral health and cognition status.\u0000Keywords: Threshold, Dental Implant Patients, Salivary Acetylcholinesterase","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"39 3","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132090845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Study the Genetic Performance of Some Faba Bean Genotypes Under MosulCondition, Iraq. 伊拉克摩苏尔条件下几种蚕豆基因型遗传性能的研究。
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/s2023.08.01.23
Esraa Abd-Alhuseein Jasim, Kamal Benyamin Esho, Nagham Salah Salim
Throughout the agricultural season fall 2020/2021, research has been carried outin the vegetable research field of the Dept. of Horticulture and Landscaping,Univ. of Mosul, to investigate the genetic performance of several Faba bean genotypes under consideration of Mosul conditions. Seeds of 7 bean cultivars weresown (S2009-175, FBSPN2, Aguadulce, ILD1814, Histal, Favada Orio, andLuzde otono). The genotypes differed considerably in all of the examined variables at a probability threshold of 5%, according to the results of an ANOVA tableanalysis of sources of variance. The genotype FBSPN2 has been superior in characteristics of plant height and seed yields regarding each of the experimentalunits. S2009-175 produced the maximum number of dry pods for each plant,Favada Orio in the trait of seed length and pod weight, and Histal produced themaximum diameter of a pod and number of seeds in each one of the pods and diameter of seed for each experimental unit. The genetic and phenotypic variancewas high for the weight of the pods for each one of the plants, the weight of 100seeds, and the height of the plant, according to the results of the genetic parameter analysis, and the percentage of heritability in a broader sense had exceeded60% for the traits, number of pods, and plant length. For the experimental unit,dry pod weight for each plant, dry pods per plant, seed diameter, pod length anddiameter, dry seed yield, and 100-seed weight were all high, and genetic improvement for pod weight, seed weight, and weight of a 100-seeds was high aswell.Keywords: Faba bean, Seed properties, Genetic parameter, Genotypes, Heritability
在整个2020/2021秋季农业季节,研究人员在北京大学园艺与园林学系的蔬菜研究领域进行了研究。研究了几种蚕豆基因型在摩苏尔条件下的遗传表现。播种了7个品种(S2009-175、FBSPN2、Aguadulce、ILD1814、Histal、Favada Orio和luzde otono)的种子。根据方差来源的方差分析表的结果,在5%的概率阈值下,所有被检查变量的基因型差异很大。FBSPN2基因型在各试验单位的株高和种子产量特征上均具有优势。S2009-175在种子长和荚果重性状上产生的单株干荚果数最多,而史达在每个试验单位的最大荚果直径、每个荚果的种子数和种子直径上产生的最大荚果数最多。遗传参数分析结果显示,单株荚果重、百粒重、株高的遗传变异和表型变异较大,单株荚果数、株长等性状的广义遗传率均超过60%。试验单位单株干荚果重、单株干荚果重、种子直径、荚果长及直径、干粒产量、百粒重均较高,籽粒重、种子重、百粒重的遗传改良也较高。关键词:蚕豆,种子特性,遗传参数,基因型,遗传力
{"title":"Study the Genetic Performance of Some Faba Bean Genotypes Under Mosul\u0000Condition, Iraq.","authors":"Esraa Abd-Alhuseein Jasim, Kamal Benyamin Esho, Nagham Salah Salim","doi":"10.21931/rb/css/s2023.08.01.23","DOIUrl":"https://doi.org/10.21931/rb/css/s2023.08.01.23","url":null,"abstract":"Throughout the agricultural season fall 2020/2021, research has been carried out\u0000in the vegetable research field of the Dept. of Horticulture and Landscaping,\u0000Univ. of Mosul, to investigate the genetic performance of several Faba bean genotypes under consideration of Mosul conditions. Seeds of 7 bean cultivars were\u0000sown (S2009-175, FBSPN2, Aguadulce, ILD1814, Histal, Favada Orio, and\u0000Luzde otono). The genotypes differed considerably in all of the examined variables at a probability threshold of 5%, according to the results of an ANOVA table\u0000analysis of sources of variance. The genotype FBSPN2 has been superior in characteristics of plant height and seed yields regarding each of the experimental\u0000units. S2009-175 produced the maximum number of dry pods for each plant,\u0000Favada Orio in the trait of seed length and pod weight, and Histal produced the\u0000maximum diameter of a pod and number of seeds in each one of the pods and diameter of seed for each experimental unit. The genetic and phenotypic variance\u0000was high for the weight of the pods for each one of the plants, the weight of 100\u0000seeds, and the height of the plant, according to the results of the genetic parameter analysis, and the percentage of heritability in a broader sense had exceeded\u000060% for the traits, number of pods, and plant length. For the experimental unit,\u0000dry pod weight for each plant, dry pods per plant, seed diameter, pod length and\u0000diameter, dry seed yield, and 100-seed weight were all high, and genetic improvement for pod weight, seed weight, and weight of a 100-seeds was high as\u0000well.\u0000Keywords: Faba bean, Seed properties, Genetic parameter, Genotypes, Heritability","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128668823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Oxytocin and Interleukin-14: New suggested indicators for prediction of type 2 diabetes-induced nephropathy 催产素和白细胞介素-14:预测2型糖尿病肾病的新指标
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.15
Rasha Hasan Jasim, Mohammed Abbas Khulaibus
Diabetes is one of the major risk factors for chronic renal failure (CRF). The present work involved studying the effect of lifestyle factors such as (age, gender,smoking and area of residence) which can affect chronic renal diseases. Also, thestudy estimated Oxytocin (Oxt) and Interleukin-14 (IL-14) using the sandwichELISA technique in the serum of type 2 diabetic patients with CRF who presented to hemodialysis and a control group. Blood samples of (50) diagnosed type 2diabetic patients with CRF from both sexes were compared with (40) samplesfrom healthy individuals as a control group. The results demonstrated significantelevation (p<0.05) in Oxt and IL-14 concentrations for patients with CRF compared to the control group. As for the subgroups, the statistical test showed a significant difference (p < 0.05) when comparing Oxt levels between the same sexin the two groups. While no significant difference was observed when comparingIL-14 in the same or the two groups. The parameters Oxt and IL-14 are necessarytools for predicting renal failure.Keywords: Oxytocin, Interleukin-14, Type 2 Diabetes Mellitus, Hemodialysis
糖尿病是慢性肾功能衰竭(CRF)的主要危险因素之一。目前的工作涉及研究生活方式因素的影响,如(年龄、性别、吸烟和居住地区),这些因素可能影响慢性肾脏疾病。此外,本研究使用三明治helisa技术估计了2型糖尿病合并CRF患者血液透析和对照组血清中的催产素(Oxt)和白细胞介素-14 (IL-14)。将50例诊断为2型糖尿病合并慢性肾功能衰竭的男女患者的血液样本与40例健康个体的血液样本作为对照组进行比较。结果显示,与对照组相比,CRF患者的Oxt和IL-14浓度显著升高(p<0.05)。亚组方面,两组同性间Oxt水平比较,经统计学检验,差异有统计学意义(p < 0.05)。而在同一组或两组中比较il -14无显著差异。Oxt和IL-14参数是预测肾功能衰竭的必要工具。关键词:催产素,白细胞介素-14,2型糖尿病,血液透析
{"title":"Oxytocin and Interleukin-14: New suggested indicators for prediction of type 2 diabetes-induced nephropathy","authors":"Rasha Hasan Jasim, Mohammed Abbas Khulaibus","doi":"10.21931/rb/css/2023.08.01.15","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.15","url":null,"abstract":"Diabetes is one of the major risk factors for chronic renal failure (CRF). The present work involved studying the effect of lifestyle factors such as (age, gender,\u0000smoking and area of residence) which can affect chronic renal diseases. Also, the\u0000study estimated Oxytocin (Oxt) and Interleukin-14 (IL-14) using the sandwich\u0000ELISA technique in the serum of type 2 diabetic patients with CRF who presented to hemodialysis and a control group. Blood samples of (50) diagnosed type 2\u0000diabetic patients with CRF from both sexes were compared with (40) samples\u0000from healthy individuals as a control group. The results demonstrated significant\u0000elevation (p<0.05) in Oxt and IL-14 concentrations for patients with CRF compared to the control group. As for the subgroups, the statistical test showed a significant difference (p < 0.05) when comparing Oxt levels between the same sex\u0000in the two groups. While no significant difference was observed when comparing\u0000IL-14 in the same or the two groups. The parameters Oxt and IL-14 are necessary\u0000tools for predicting renal failure.\u0000Keywords: Oxytocin, Interleukin-14, Type 2 Diabetes Mellitus, Hemodialysis","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"18 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114095475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Polymerization of sunflower oil with adipic acid using Catalyst atdifferent temperatures 向日葵油与己二酸在不同温度下的聚合反应
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.91
Safa fakhri Kadouri, Ghalib Adrees Atiya Ali, Abdul Muniem Abbas Karim
The polymers that have been prepared include the following:Poly(glyceride-ALT-adipate), which symbolizes it, has been prepared in twosteps: The first step: Prepare the monomer from the reaction of glycerin with sunflower oil (glyceride reaction) in the presence of catalysts (pbO2) at a temperature of 150 and using the Electrical Stirrer for 5 hours. The second step: This stepincludes the polymerization process (condensation) in order to prepare (AlkydResin), where the monomer (Monoglyceride) is prepared by reacting with (Adipic acid) at different temperatures starting from (150 to 240 with a difference of 10℃ between one reaction and another using an electronic thermometer to controlthe temperature and with continuous stirring using a magnet bar and in the presence of nitrogen gas (inert atmosphere). The reaction kinetics is also calculated,which includes two processes. The first process, the kinetics of the reaction, ismade by tracking the decrease in the concentration of acid value, which is madeby the polymerization process.Keywords: sunflower oil, adipic acid, PbO2 Catalyst.
所制备的聚合物包括:聚(甘油三酯- alt -己二酸酯),表示它,已分为两步制备:第一步:在催化剂(pbO2)存在下,将甘油与葵花籽油反应(甘油三酯反应)在150℃下,使用电动搅拌器搅拌5小时,制备单体。第二步:这一步包括聚合过程(冷凝),以制备(醇酯树脂),其中单体(单甘油酯)是通过与(己二酸)在不同的温度下(150至240)反应而制备的,每次反应之间相差10℃,使用电子温度计控制温度,并使用磁铁棒在氮气(惰性气氛)存在下连续搅拌。计算了反应动力学,包括两个过程。第一个过程,即反应动力学,是通过跟踪聚合过程中酸值浓度的下降来实现的。关键词:葵花籽油,己二酸,PbO2催化剂
{"title":"Polymerization of sunflower oil with adipic acid using Catalyst at\u0000different temperatures","authors":"Safa fakhri Kadouri, Ghalib Adrees Atiya Ali, Abdul Muniem Abbas Karim","doi":"10.21931/rb/css/2023.08.01.91","DOIUrl":"https://doi.org/10.21931/rb/css/2023.08.01.91","url":null,"abstract":"The polymers that have been prepared include the following:\u0000Poly(glyceride-ALT-adipate), which symbolizes it, has been prepared in two\u0000steps: The first step: Prepare the monomer from the reaction of glycerin with sunflower oil (glyceride reaction) in the presence of catalysts (pbO2) at a temperature of 150 and using the Electrical Stirrer for 5 hours. The second step: This step\u0000includes the polymerization process (condensation) in order to prepare (Alkyd\u0000Resin), where the monomer (Monoglyceride) is prepared by reacting with (Adipic acid) at different temperatures starting from (150 to 240 with a difference of 10\u0000℃ between one reaction and another using an electronic thermometer to control\u0000the temperature and with continuous stirring using a magnet bar and in the presence of nitrogen gas (inert atmosphere). The reaction kinetics is also calculated,\u0000which includes two processes. The first process, the kinetics of the reaction, is\u0000made by tracking the decrease in the concentration of acid value, which is made\u0000by the polymerization process.\u0000Keywords: sunflower oil, adipic acid, PbO2 Catalyst.","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125286603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biological activities of purified prolidase from pathogenic E.coli 致病性大肠杆菌纯化脯氨酸酶的生物学活性
Pub Date : 2023-08-15 DOI: 10.21931/rb/css/2023.08.01.57
Seror A. Abdul Hussein, Neihaya H. Zaki, Khetam H.Rasool
Prolidase is a ubiquitous enzyme that plays a chief role in the metabolism of proline-rich proteins. The goal of this study extraction and purifyprolidase from E. coli and evaluate some applications such as anti-biofilm andanticancer. Hundred stool Samples were collected from infants with breastfeeding, non-vomiting, and non-diarrhea to isolate E.coli bacteria. A 16S rRNA gene(585 bp) was found in all isolates of E. coli via PCR identification. Depending onthe qualitative method on prolidase agar, only 40 (80%) isolates could produceprolidase from 50 isolates that were considered non-pathogens, then only 32 isolates revealed different levels in prolidase production with specific activity equalto (2.1U/mg) of E.coli.MS12. Sucrose, casein, and 40Co were the chosen isolate's best conditions forproducing prolidase. Cold acetone precipitation and dialysis were used to extractthe enzyme, and DEAE-cellulose and the Sephadex G-150 column were used inpurification with specific activity (2 U/ml) and (6.6 U/mg) protein. Prolidaseshowed the highest effect on biofilm at 500 μg/ml concentration against P. aeruginosa, then E. coli, 65% and 60.3% respectively. Brain Tumor Cell Line (A127),Colorectal Adenocarcinoma cells (CaCo-2), and Normal embryonic liver cell line(WRL-68) were used to test the prolidase effect on these cell lines. An assay ofMTT was used to detect the inhibiter concentration (IC50) values and cytotoxiceffect of purified prolidase.Keywords: E.coli, Prolidase, purification, antibiofilm, anticancer activity
脯氨酸酶是一种普遍存在的酶,在富含脯氨酸的蛋白质的代谢中起主要作用。本研究的目的是从大肠杆菌中提取纯化脯氨酸酶,并评价其在抗生物膜和抗癌等方面的应用。收集了100例母乳喂养、无呕吐、无腹泻婴儿的粪便样本,以分离大肠杆菌。所有分离株经PCR鉴定均含有16S rRNA基因(585 bp)。根据在增殖酶琼脂上的定性方法,50株被认为是非病原体的分离株中只有40株(80%)能产生增殖酶,而只有32株显示出不同水平的增殖酶,比活性相当于大肠杆菌ms12 (2.1U/mg)。蔗糖、酪蛋白和40Co是所选菌株产生脯氨酸酶的最佳条件。采用冷丙酮沉淀法和透析法提取酶,deae -纤维素和Sephadex G-150柱纯化,比活性为2 U/ml,蛋白为6.6 U/mg。在500 μg/ml浓度下,增殖酶对铜绿假单胞菌(P. aeruginosa)、大肠杆菌(E. coli)的生物膜作用最大,分别为65%和60.3%。以脑肿瘤细胞系(A127)、结直肠癌细胞系(CaCo-2)和正常胚胎肝细胞系(WRL-68)为实验对象,研究了脯氨酸酶对这些细胞系的影响。采用mtt法检测纯化后的酶抑制剂浓度(IC50)值和细胞毒作用。关键词:大肠杆菌,脯氨酸酶,纯化,抗菌膜,抗癌活性
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