In Vitro Cytotoxicity Studies and Antioxidant activities of two wild yams

Abstract

Biomedical application of Dioscorea pentaphylla and Dioscorea oppositifolia by determining the cytotoxicity and antioxidant properties of tuber extracts. Plant extracts expressed cytotoxicity against human lung carcinoma cell line (A-549) which was ascertained by MTT Assay. From this the D. pentaphylla tuber was found to be more effective on (A-549) cell line. The present work also designed the antioxidant activities of D. pentaphylla and D. oppositifolia methanol extract were assessed by using DPPH and ABTS + activity. In DPPH activities among the five different concentrations (100µg/ml to 500µg/ml) of extracts tested. In ABTS + activity D. pentaphylla and D. oppositifolia were assayed by using five different concentrations (50µg/ml to 250µg/ml). The absorbance was increased in both assays with increasing concentrations of the extracts.