Seroprevalence of toxoplasmosis related with uric acid in B-thalassemia major patients

Abstract

Laboratory experiments were performed to study 66 samples were collected for beta-thalassemia patients, with 30 samples for control of healthy people, male and female, and 96 samples were examined in the Cobas e411 Advice for both IgG and IgM, 20 samples were obtained positive for IgG in patients with betathalassemia, and one sample was positive for IgM, then the Uric Acid examination was conducted for all After that, 12 male and female samples were selected with a high percentage of Uric Acid, 6 of which were positive for Toxoplasmosis and 6 negatives for the disease. To perform the Performance Liquid Chromatography (HPLC) technique and use the standard substance allantoin to observe the relationship between Uric Acid and Allantoin, where the relationship is inverse between them; in the case of infection and the presence of the parasite in the blood, the Uric Acid oxidizes and combines with the free radicals (reactive oxygen species) in the blood such as oxygen and hydrogen peroxide that are harmful to the cell or tissue. Free radicals in abundance and as a guide to the extent of damage performed by the parasite. Keywords: beta-thalassemia, Uric Acid, Allantoin, Toxoplasmosis, IgG and IgM.