Evaluation of the Antibacterial Effect of the Prepared Graphene Oxide Nanosheets

Abstract

Because of its exceptional chemical, physical, and biological features, graphene oxide (GO) has gained great attention. The Simplified Hammer's method was used in this study to synthesize GO nanosheets, which were then characterized by UV-Vis, SEM, FTIR spectroscopy, and XRD analysis. GO's UV-Vis peak of absorption was at 230 nm. A peak of diffraction at 2θ= 10.75° was found in the XRD examination, equivalent to around 0.82 nm spacing among layers. In the FTIR spectra, characteristic peaks associated with oxygen functional groups of GO were identified. In SEM pictures, GO is made up of crumpled thin nanosheets. The diffusion in agar well procedure was applied to investigate the effect of as-prepared GO nanosheets against gram-negative "Pseudomonas aeruginosa, Salmonella typhi" and gram-positive "Enterococcus sp., Kocuria sp." Different concentrations of GO demonstrated potent antibacterial activity, with higher efficacy against gram-positive bacteria. Keywords: Graphene Oxide, Nanosheets, Antibacterial effect, XRD, SEM, FTIR.