Histopathology of Kidney In Mice (Mus musculus L) Indicated By Pre-Diabetes Mellitus Type 2 (Pre-Type 2 DM) With a variation of Yeast Treatment

Abstract

— A kidney is an organ that has many roles to survive. This organ can be damaged by the type 2 diabetes mellitus which is marked by high glucose in the blood. Histological studies of kidney tissue in mice ( Mus musculus L .) shown by type 2 pre-Diabetes Mellitus with variations in yeast treatment have been carried out. This study uses variations in the treatment of yeast planted in red rice (red yeast) and yeast extract produced by enzymatic hydrolysis (YHE-red rice) as a comparison. Mice as the experimental animals were induced obesity using a high-fat and fructose diet, so they showed symptoms of Pre-DM type 2 and were then treated with red yeast rice and red YHE-rice. This study used a comparison of DM mice and treated mice with glibenclamide in CMC-Na 0.2% (positive control), DM mice and treated mice with CMC-Na 0.2% (negative control), and non-DM mice and treated mice with CMC -Na 0.2% (normal control). Observation of renal histopathology using a light microscope. Observed Parameters included cell necrosis, glomerular nephropathy performance, increasing of Bowman's space thickness, thickening of the distal and proximal tubules, thickening of the renal arteries, and leukocyte infiltmiceion. The results showed that the kidneys of type-2 DM mice and Yeast-Red Rice and Yeast Extract-Red Rice as well as mice that indicated DM and were treated with glibenclamide in 0.2% CMC-Na for 1 week, did not show histological differences. Kidney when compared with non-DM mice and treated with 0.2% CMC-Na (normal control), as well as mice that were indicated by DM and treated with 0.2 CMC-Na. So to find out the effect of yeast treatment on the kidney of mice indicated by type -2 DM, it is necessary to induce obesity and treat yeast-red rice or YHE-red rice for longer periods.