Curing of Resistant Pseudomonas aeruginosa by Ethidium Bromide

Abstract

The Pseudomonas aeruginosa bacteriocin represents one of the survival methods in the mixed communities. It had its structure that gathered between colicin, which had enzymatic activity, and the pore-former toxin. Bacteriocins represent a way to gain the battle against the immune system. Antibiotics were the most effective therapy, but with time and random usage, antibiotic resistance has been developed and spread worldwide. In this study, ten isolates of Pseudomonas aeruginosa were detected for bacteriocin production against three Escherichia coli isolates. Pseudomonas aeruginosa isolates were bacteriocin producer. After that, curing by four concentrations (50, 75,100 and 125) µg /ml of Ethidium bromide was done. The (75 and 100) µg /ml were the most effective concentration in curing. The Antibiotic sensitivity test for the antibiotics was done before and after curing in order to make a combination between bacteriocin production and curing as a way to gain the battle against antibiotic resistance. This study proved that the anti-plasmid factor could be considered a promising way to deal with the progress of the spread of antibiotic resistance in the community. Keywords: Pseudomonas aeruginosa, bacteriocin, curing and antibiotic resistance.