Exploration of Antibacterial Compounds from the Commiphora myrrha Through GC-MS and Their Evaluation Against the Bacteria Isolated from Corvus splendens

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Abstract

The Corvus splendens (Indian house crow) normally fed on the garbage therefore, they have many pathogenic bacteria, which can infect the human and cause severe infection in human irrespective to the gender, age and region. The bacteria samples were isolated from the faeces, blood lever, and intestines of the crows in Luria-Bertani (LB) agar plates. The culture plates were incubated at 37°C until the colonies were appeared. The colonies were identified morphologically and through molecular marker using 16S-rDNA. The Commiphora myrrha plant extract was used to determine the antibacterial activities against the pathogenic bacteria isolated from crows. The plant was crushed with 70% methanol and filtered. The filtrates were dried and dissolved in 100% methanol. Antibacterial activities of plant extracts were determined against the pathogenic bacteria isolated from crows. The results showed that the plant extracts were very effective against the pathogenic bacteria and showed 100% antibacterial activities. The plants extracts were analyzed for the characterization of different antimicrobial compound through gas chromatography mass spectrometry (GC-MS). The undecane, tetrasiloxane, hexadecanoic acid, heptasiloxane, benzocyclohepten and many other compounds. The antibacterial activities of the plant extracts were might be because of their compound which had been reported previously as well as an antimicrobial compound.
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气相色谱-质谱法研究没药中抗菌化合物及其对锦绣Corvus splendens细菌的抑制作用
印度家鸦(Corvus splendens)通常以垃圾为食,因此它们有许多致病菌,可以感染人类,不分性别、年龄和地区,都能引起人类的严重感染。用LB琼脂平板从乌鸦的粪便、血杠杆和肠道中分离细菌。培养板37℃孵育,直至菌落出现。利用16S-rDNA对菌落进行形态鉴定和分子标记。采用紫薇植物提取物对乌鸦致病菌进行抑菌活性测定。该植物用70%的甲醇粉碎并过滤。将滤液干燥并溶解在100%甲醇中。测定了植物提取物对乌鸦致病菌的抑菌活性。结果表明,该植物提取物对病原菌具有良好的抑菌活性,抑菌活性达到100%。采用气相色谱-质谱(GC-MS)对植物提取物中不同抑菌成分进行分析。有十一烷、四硅氧烷、十六烷酸、七硅氧烷、苯并环庚烯等许多化合物。该植物提取物具有抗菌活性,可能是由于其含有已被报道过的化合物和抗菌化合物。
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