PSEUDOMONAS AERUGINOSA AND STAPHYLOCOCCUS AUREUS PROFILE IN SOME DAIRY FARMS

Shimaa Hassanein, W. Elsherif, Mohamed A. Elshater, M. Sayed
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Abstract

The purpose of the current study was to evaluate the degree of contamination of dairy farms with both Pseudomonas aeruginosa and Staphylococcus aureus , in which, a total of 150 swab samples were collected from 3 different dairy farms (50 each) distributed in Assiut governorate (farm A) and El-Minia governorate (farms B & C), Egypt. The swabs were taken from different locations in the dairy farms such as teats of dairy animals, milking machines, workers' hands and stanchions, etc. All the samples were examined for detection of Ps. aeruginosa (conventionally biochemical followed by MICROBACT™ identification kits) and for Staph. aureus (coagulase +ve). The obtained results revealed the overall incidence of Ps. aeruginosa as 14%, 2% and 4% in farms A, B & C, respectively; while, the incidence of Staph. aureus was 4%, 30% and 4%, respectively. In conclusion, the profile of both Ps. aeruginosa and Staph. aureus among the examined dairy farms is highlighting the hygienic state of each farm separately.
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铜绿假单胞菌和金黄色葡萄球菌分布在一些奶牛场
本研究的目的是评估铜绿假单胞菌和金黄色葡萄球菌对奶牛场的污染程度,其中,从分布在埃及Assiut省(农场a)和El-Minia省(农场B和C)的3个不同的奶牛场(每个农场50个)共收集了150份棉絮样本。这些棉签取自奶牛场的不同位置,如奶牛的乳头、挤奶机、工人的手和支柱等。对所有样品进行铜绿假单胞菌(Ps. aeruginosa)和葡萄球菌(Staph)的检测(常规生化检测,随后采用microbat™鉴定试剂盒)。金黄色(凝固酶+ve)。结果表明,A、B、C养殖场铜绿假单胞菌总发病率分别为14%、2%和4%;同时,葡萄球菌的发病率。金黄色葡萄球菌分别为4%、30%和4%。综上所述,铜绿假单胞菌和葡萄球菌的基因图谱均为。在被检查的奶牛场中,金黄色葡萄球菌的出现突出了每个奶牛场的卫生状况。
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