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Bull sperm rheotaxis and kinematics in microfluidic channels with different heights 公牛精子在不同高度微流体通道中的流变和运动学特性
Pub Date : 2023-12-23 DOI: 10.21608/avmj.2023.228274.1176
Haitham Mofadel, Hassan Ali Hussein, Ahmed Fath El-Bab, Taymour El‑Sherry
Recently, microfluidics has attracted a lot of attention in reproduction due to its ability to simulate the natural environment inside the female reproductive tract and its ability to separate high-quality sperm cells using rheotaxis as a selection mechanism. Positive rheotaxis refers to the ability of the sperm to direct itself and swim against the liquid current during the fertilization journey. In this study, we investigated the effects of various microchannel heights on sperm positive rheotaxis (PR) and sperm kinematics. We used two microchannels with the same width (200µm) and different heights (channel 1= 20 µm height and channel 2= 100 µm height). Sperm samples were obtained from two bulls with known fertility and analyzed using CASA. The results showed that PR and all sperm kinematics (VCL, VSL, VAP
最近,微流控技术在生殖领域引起了广泛关注,因为它能够模拟女性生殖道内的自然环境,并能利用流变性作为一种选择机制来分离优质精子细胞。正向流变性是指精子在受精过程中逆流而上的能力。在这项研究中,我们研究了不同微通道高度对精子正向流变性(PR)和精子运动学的影响。我们使用了两个宽度相同(200 微米)、高度不同(通道 1= 20 微米高,通道 2= 100 微米高)的微通道。精子样本取自两头已知繁殖力的公牛,并使用 CASA 进行分析。结果显示,PR 和所有精子运动学指标(VCL、VSL、VAP
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引用次数: 0
Effect of Packaging Method and Use of Acetic Acid on the Shelf Life of Fish During Refrigeration Storage 包装方法和醋酸的使用对鱼类冷藏保质期的影响
Pub Date : 2023-12-23 DOI: 10.21608/avmj.2023.231759.1181
Mohamed El Asuoty
This experiment was conducted to evaluate the efficacy of application of acetic acid 1%, non-vacuum packaging and vacuum packaging on the shelf-life of tilapia fish filets at refrigerated temperature (2±1 o C) during 21 days of storage period. In this regard, the current study investigated the quality attributes including sensory, TBA, TVB-N, pH and microbial loads of examined fish. Vacuum packing and acetic acid were found to possess potent antibacterial effects on a variety of microorganisms. The fish were divided into four groups after being gutted and filleted: Group A (GA): fish fillets were vacuum packaged in Polyamide/ Polyethylene (PA/PE) bags. Group B (GB): the fillets were stored in non-vacuum containers. Group C (GC): the fillets underwent chemical treatment by immersion in 1% acetic acid for 2 minutes at room temperature, drained for two minutes then vacuum packaged in Polyamide/ Polyethylene (PA/PE) bags. Group D (GD): the fillets treated as in GC stored in non-vacuum containers. All groups were stored at 2±1 o C, 80% CO 2 and 20% N 2 for 21 days. The experiment was repeated in triplicate. The sensory analysis of fish fillets revealed that samples in group C which were treated with acetic acid 1% in combination with vacuum packaging during storage for 21 days at 2±1 o C had the best sensory properties and the best shelf life, and a low pH value reduced or even limited microbial levels, and ensured the safety of different microbial counts as well as reduction of TBA and TVB-N in acetic acid-treated groups compared with other groups. Together with the sensory characteristics, the low or limited microbial counts (total viable count, total psychrotrophic count, and total coliform counts) suggested that autolysis or other causes other than microbial activity may be to blame for the deterioration. Meanwhile, the shelf-life of fish treated with vacuum packaging had a longer shelf-life than that treated with non-vacuum packaging. Overall, the research underscores the need for more research and development to produce seafood that is stable and safe against microbes and has a long shelf life in order to meet customer demand.
本实验旨在评估 1%醋酸、非真空包装和真空包装对罗非鱼片在冷藏温度(2±1 o C)下储存 21 天的货架期的影响。为此,本研究调查了受检鱼肉的感官、TBA、TVB-N、pH 值和微生物负荷等质量属性。研究发现,真空包装和醋酸对多种微生物有很强的抗菌作用。将鱼去内脏和切片后分为四组:A 组(GA):鱼片用聚酰胺/聚乙烯(PA/PE)袋真空包装。B 组(GB):鱼片储存在非真空容器中。C 组(GC):鱼片经过化学处理,在室温下浸泡在 1%的醋酸中 2 分钟,沥干 2 分钟后用聚酰胺/聚乙烯(PA/PE)袋真空包装。D 组(GD):在非真空容器中储存的鱼片,处理方法与 GC 相同。所有组别均在 2±1 o C、80% CO 2 和 20% N 2 条件下储存 21 天。实验一式三份。鱼片的感官分析表明,在 2±1 o C 下贮存 21 天期间,用 1%醋酸处理并结合真空包装的 C 组样品具有最佳的感官特性和最佳的货架期,与其他组相比,低 pH 值降低甚至限制了微生物水平,确保了不同微生物计数的安全性,并降低了醋酸处理组的 TBA 和 TVB-N。从感官特征来看,微生物数量(总存活数、总精神营养数和总大肠菌群数)较低或有限,这表明自溶或微生物活动以外的其他原因可能是导致变质的原因。同时,采用真空包装处理的鱼的货架期要长于非真空包装处理的鱼。总之,这项研究强调,为了满足客户的需求,有必要进行更多的研究和开发,以生产对微生物稳定、安全且货架期长的海产品。
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引用次数: 0
Effect of different Thawing methods on sensory, physico – chemical characteristics of imported frozen beef 不同解冻方法对进口冷冻牛肉感官和理化特性的影响
Pub Date : 2023-12-23 DOI: 10.21608/avmj.2023.227868.1177
Sara kassem, Ashraf Abdel-malek, Shaymaa El sayed
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引用次数: 0
Molecular Analysis of Field Infectious Bursal Disease Virus Isolated in Sulaimani/ Iraq. 伊拉克苏莱曼尼分离到的野外传染性法氏囊病病毒的分子分析。
Pub Date : 2023-12-23 DOI: 10.21608/avmj.2023.219891.1160
hana raoof, mohammed babasheikh
Infectious Bursal Disease (IBD) is also known as Gumboro disease, where the illness was initially identified. It is an extremely transmissible immune suppressor virus that has a significant financial impact on the poultry sector around the world resulting in increased vulnerability to various infections, adding to its negative effects on the efficacy of vaccines. The very virulent infectious bursal disease (vvIBDV) strains that are extremely virulent and cause a large amount of fatality in hens have emerged in recent years. The present study used reverse transcription polymerase chain reaction (RT-PCR) followed by sequencing to detect and molecularly characterize the VP1 and VP2 genes of IBDV. A phylogenetic study of the partial VP2 gene revealed that three field isolates belonged to Genogroup 3 and clustering Poland, Hong Kong, and Iran, with the highest identities of 98.32% and 98%, respectively. Based on an analysis of the amino acid sequence deduced from the VP2 gene at positions 242I, 253Q, 256I, 272I, 279D, 284A, 294I, 299S, and 330S, three IBDVs were shown to be very virulent strains, however, amino acid analysis of the VP1 in these two isolates showed that one of them had the distinctive vvIBDV TDN amino acid triplet, while the other isolates had a non-vIBDV HEG amino acid triplet at positions 145/146/147, respectively. The VP2 protein sequence of the circulatory vvIBDV genotype showed heterogeneity of a few amino acid substitutions within the hypervariable region with the vaccine strains ((Bursin, Cevac, and D78) that are commonly used in the vaccination program in Iraq. Conclusion: The current investigation might document the detection of highly virulent IBD virus from local farms during the outbreaks that emerged in the Sulaimani province. These data suggest that the current vaccination failure may be related to differences in the VP2 protein at HVR of the regionally circulating IBDV strain.
传染性法氏囊病(IBD)也被称为冈波罗病,这种疾病最初是在冈波罗被发现的。它是一种传播性极强的免疫抑制病毒,对全球家禽业产生了重大的经济影响,导致家禽更容易受到各种感染,并对疫苗的效力产生了负面影响。近年来出现了毒性极强的传染性法氏囊病(vvIBDV)毒株,其毒性极强,可导致大量母鸡死亡。本研究利用反转录聚合酶链反应(RT-PCR)和测序技术检测了 IBDV 的 VP1 和 VP2 基因,并对其进行了分子鉴定。对部分 VP2 基因的系统发育研究表明,3 个野外分离株属于基因组 3,分别聚类于波兰、香港和伊朗,最高相同度分别为 98.32% 和 98%。然而,根据对 VP2 基因 242I、253Q、256I、272I、279D、284A、294I、299S 和 330S 位点的氨基酸序列推导分析,这三种 IBDV 均为毒性很强的毒株、对这两个分离株的 VP1 进行氨基酸分析表明,其中一个分离株具有独特的 vvIBDV TDN 氨基酸三连体,而另一个分离株在 145/146/147 位分别具有非 vvIBDV HEG 氨基酸三连体。循环型 vvIBDV 基因型的 VP2 蛋白序列显示,与伊拉克疫苗接种计划中常用的疫苗株(Bursin、Cevac 和 D78)相比,在高变异区内存在一些氨基酸替代的异质性。结论目前的调查可能记录了在苏莱曼尼省爆发疫情期间从当地农场检测到的高致病性 IBD 病毒。这些数据表明,目前的疫苗接种失败可能与该地区流行的 IBDV 株 HVR 的 VP2 蛋白差异有关。
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引用次数: 0
EFFECTS of BORON SUPPLEMENTATION on DAIRY CALVES’ HEALTH: A METABOLOMICS STUDY 补充硼元素对奶牛健康的影响:代谢组学研究
Pub Date : 2023-12-23 DOI: 10.21608/avmj.2023.218730.1157
A. Basoglu, Adam ABDEL-AZIZ ADAM, N. Başpınar, A. Vignoli, G. Meoni, L. Tenori, Erdem Gullersoy, Rumeyhisa Bicici
Boron supplementation has been demonstrated to exert many beneficial effects on animals. The first metabolomic investigation into the effects of boron on dairy calf health is covered in this study. For two months, healthy newborn calves were enrolled in one control group and three experimental groups. Boron was added to the milk of the experimental groups in increasing doses. Biochemical profile, Nuclear Magnetic Resonance (NMR) metabolomic profiles on days 0, 30, and 60, and daily health scores were determined. Worsening of health score parameters was more common in the control calves
事实证明,补充硼元素对动物有许多有益的影响。本研究首次对硼对乳牛健康的影响进行了代谢组学调查。在两个月的时间里,健康的新生犊牛被分为一个对照组和三个实验组。实验组的牛奶中添加的硼剂量不断增加。测定第 0、30 和 60 天的生化概况、核磁共振(NMR)代谢组概况以及每日健康评分。对照组犊牛的健康评分参数更容易恶化
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引用次数: 0
Studying caecotrophy behaviour (complete and censored) in three rabbit breeds at four seasons using different survival techniques 利用不同的生存技术研究三个兔种在四个季节的粪便营养行为(完整和删减
Pub Date : 2023-11-29 DOI: 10.21608/avmj.2023.224964.1170
Heba Gharib, fatma Abdallah
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引用次数: 0
VINCRISTINE-INDUCED NEUROTOXICITY IN RATS MEDIATED BY UPREGULATION OF INOS, IBA1, NESTIN, PARP AND CASPASE 3: AMELIORATIVE EFFECT OF ERYTHROPOIETIN AND THYMOQUINONE 长春新碱诱导大鼠inos、iba1、nestin、parp和caspase 3上调的神经毒性:促红细胞生成素和百里醌的改善作用
Pub Date : 2023-10-01 DOI: 10.21608/avmj.2023.223481.1171
SAHAR AHMED, HOSSAM EL-DIN OMAR, MAHMOUD SOLIMAN, ABO BAKR ABDEL SHAKOR, MANAL SAYED, OMNIA OMAR, SARY ABD ELGHAFFAR
Vincristine (VCR) is an effective anticancer medication, although it has neurotoxic side effects. Erythropoietin (EPO) is the main regulator of erythropoiesis. Thymoquinone (TQ) protects brain cells from oxidative stress that causes neurodegenerative disorders such as Alzheimer's and Parkinson's. This study aims to investigate the VCR toxicity on the cerebrum as well as the possible neuroprotective effects of TQ and EPO against VCR toxicity in a rat model. An intraperitoneal injection of VCR (150 μg/kg) 3 times a week for 5 weeks caused marked histopathological changes in the brain such as neuronal degeneration with aggregations of glial cells around the degenerated neurons (satellitosis), congestion of blood vessels and severe demyelination in the white matter of the cerebrum. VCR considerably increased nestin, iBA1 and iNOS expression, while synaptophysin expression decreased. It also caused upregulation of caspase 3 and PARP expression, resulting in hemorrhage, demyelination, and neuronal degeneration. Treatment of rats with TQ or EPO either alone or in combination improved histopathological changes through down-regulation of nestin, iBA1, iNOS, caspase 3 and PARP. It was concluded that EPO and TQ ameliorate the neurotoxic effect of VCR on the cerebrum, however, a synergetic effect was evident when TQ and EPO were combined.
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引用次数: 0
EFFECTS OF AQUEOUS EXTRACT OF Miswak (SALVADORA PERSICA) ON HISTOMORPHOLOGICAL STRUCTURE OF THE UTERUS IN THE FEMALE RATS 山菖蒲水提物对雌性大鼠子宫组织形态结构的影响
Pub Date : 2023-10-01 DOI: 10.21608/avmj.2023.214155.1149
SABREEN GHAREEB, MAHMOUD ABD-ELKAREEM, AHMED ABOU-ELMAGD
The uterus is a very important reproductive organ for implantation and holding embryos till labor. Many drugs and medicinal plants affect the uterus, some researchers reported that Salvadora persica (Miswak) is a phytoestrogen plant as it contains flavonoids and can be used as a contraceptive drug; however, no available investigations explain the histomorphological structure of the uterus after Miswak extract administration. Twelve female albino rats (165.3 ± 3.269 g) were divided equally into two groups. In the control group, the animals received normal saline daily for 4 weeks. While in the Miswak treated group, the animals received 900 mg/kg of body weight of the Miswak aqueous extract daily for the same period. Grossly, at the end of the experiment, our results revealed that the uteri in the Miswak-treated group had characteristics of low active organs; they were pale with low weight and high thickness. In contrast to the treated group, the uteri of the control group had the signs of active organs; they were more vascular, relatively thin and edematous. Microscopically, in the control group, the uterine lumen was wide and the mucosa was folded and covered by secretory columnar epithelium. The uterine glands were more active, and the myometrium was thick. Whereas, in the Miswak-treated group, the lumen was slit-like, the uterine glands were less active, and the uterine wall had thick lamina propria and thin myometrium. In conclusion, the oral administration of Miswak extract reduces the uterus activity. It showed a state of anestrous with a narrow slit-like lumen and a decrease in glandular activity.
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引用次数: 0
RETROSPECTIVE STUDIES ON DYSTOCIA IN EWES: CAUSES AND TREATMENT BY CESAREAN SECTION 母羊难产的回顾性研究:原因及剖宫产治疗
Pub Date : 2023-10-01 DOI: 10.21608/avmj.2023.220195.1161
TALIB MNNAT ALI
The objectives of the present study are to know the main causes of dystocia. A total of twenty-two native sheep suffered from dystocia. Cases were treated in the veterinary hospital/ teaching hospital / University of Sulaimani. Out of twenty-two sheep suffered from dystocia, 14(63.6%) resulted from fatal causes and 8(36.4%) from maternal causes. Fetal status represented by 4(28.6%), 2(14.4%), 3(21.4%), 1(7.1%), 3(21.4%) and 1(7.1%), for schistosomus reflexus, oversize, head deviation, breach presentation, bilateral shoulder flection and transverse presentation, respectively. While maternal causes are classified as, 4 (50%) for ring womb, 2(25%) for uterine prolapse and 2(25%), for narrow pelvis. Parity, sex of the fetus and viability of the fetus are considered predisposing factors to cause dystocia in sheep. All Emergency cases of dystocia are treated through cesarean section. The sequel of postoperative surgical operation is represented by 3(13.6%), 7(31.8%), 2(8%), and 1(4.5%), for retained placenta, metritis, uterine prolapse and bleeding, respectively. Whereas, the effect of cesarean section on the fertility of sheep is represented by 8(42.1), and 11(57.9%), for pregnant and non-pregnant, respectively. In addition, the mortality rate of sheep in the study reached 3(13.6%), because of postpartum uterine infections. In conclusion, generally dystocia in sheep results from fetal and maternal causes, and due to prolonged time of labor the early intervention by cesarean section could be for saving the lambs and the dam.
{"title":"RETROSPECTIVE STUDIES ON DYSTOCIA IN EWES: CAUSES AND TREATMENT BY CESAREAN SECTION","authors":"TALIB MNNAT ALI","doi":"10.21608/avmj.2023.220195.1161","DOIUrl":"https://doi.org/10.21608/avmj.2023.220195.1161","url":null,"abstract":"The objectives of the present study are to know the main causes of dystocia. A total of twenty-two native sheep suffered from dystocia. Cases were treated in the veterinary hospital/ teaching hospital / University of Sulaimani. Out of twenty-two sheep suffered from dystocia, 14(63.6%) resulted from fatal causes and 8(36.4%) from maternal causes. Fetal status represented by 4(28.6%), 2(14.4%), 3(21.4%), 1(7.1%), 3(21.4%) and 1(7.1%), for schistosomus reflexus, oversize, head deviation, breach presentation, bilateral shoulder flection and transverse presentation, respectively. While maternal causes are classified as, 4 (50%) for ring womb, 2(25%) for uterine prolapse and 2(25%), for narrow pelvis. Parity, sex of the fetus and viability of the fetus are considered predisposing factors to cause dystocia in sheep. All Emergency cases of dystocia are treated through cesarean section. The sequel of postoperative surgical operation is represented by 3(13.6%), 7(31.8%), 2(8%), and 1(4.5%), for retained placenta, metritis, uterine prolapse and bleeding, respectively. Whereas, the effect of cesarean section on the fertility of sheep is represented by 8(42.1), and 11(57.9%), for pregnant and non-pregnant, respectively. In addition, the mortality rate of sheep in the study reached 3(13.6%), because of postpartum uterine infections. In conclusion, generally dystocia in sheep results from fetal and maternal causes, and due to prolonged time of labor the early intervention by cesarean section could be for saving the lambs and the dam.","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135759922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Studies on renal bacterial affection in sheep 绵羊肾脏细菌影响的研究
Pub Date : 2023-09-26 DOI: 10.21608/avmj.2023.218387.1156
Jakleen Tawfik
{"title":"Studies on renal bacterial affection in sheep","authors":"Jakleen Tawfik","doi":"10.21608/avmj.2023.218387.1156","DOIUrl":"https://doi.org/10.21608/avmj.2023.218387.1156","url":null,"abstract":"","PeriodicalId":299161,"journal":{"name":"Assiut Veterinary Medical Journal","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134886109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
期刊
Assiut Veterinary Medical Journal
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