The Potential Effect of Ethyl Acetate fraction of Tephrosia Purpurea Linn Leaves (tpeaf) and Rutin in FCA induced Arthritis in experimental Animals

Abstract

The aim of the present study is to investigate the anti arthritic activity of ethyl acetate fraction Tephrosia purpurea Linn leaves (TPEAF) at the dose of 200 and 400 mg/kg body weight and Rutin at the dose of 100 mg/kg body weight on FCA induced arthritis in experimental rats. Arthritis was induced by injecting a 0.1 ml suspension of killed Mycobacterium tuberculosis bacteria (0.1% w/v) homogenized in liquid paraffin (Freund’s complete adjuvant, FCA) into the right hind paw. Evaluation parameters such as Arthritic index, Paw edema was determined on respective days of experiment and pain perception parameters such as dorsal flexion pain, Stair climbing activity, and Motility score was recorded. The biochemical parameters like serum transaminases, hematological parameters, CRP (Creactive protein), RF (Rheumatoid factor) level were determined. From all the investigations it has been observed that TPEAF and Rutin showed significantly decreased in paw edema. The altered hematological parameters (Hb, RBC, WBC, ESR, CRP, and RP) in the arthritic rats were significantly recovered to normal by administration of TPEAF and Rutin. Further, the radiological studies revealed TPEAF and Rutin showed anti-arthritic activity by indicating less abnormality in bone when compared to the disease control group. Findings from the present investigations showed that the TPEAF and Rutin exhibit significant anti-arthritic activity.