Biological Cell Characterization and Discrimination Based on UHF-Dielectrophoresis for Next Generation of Liquid Biopsy Analysis

E. Lambert, Elodie Barthout, R. Manczak, S. Saada, Lea Ikhlef, R. Formento, M. Verdier, C. Dalmay, B. Bessette, F. Lalloué, A. Pothier
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Abstract

This paper introduces results about characterizations of different tumor cell lines, using intracellular sensing based on Ultra High Frequency dielectrophoresis (UHF-DEP). Above 50 MHz, presented technic allows to probe internal cell content to characterize its cytoplasm permittivity and conductivity properties. The measured frequency-dependent cell behavior results on a distinctive DEP signature according to the cell type and its biological specificity. This paper illustrates the high potential of UHF lab-on-chip sensor to discriminate various tumor cell types derived from different tissues.
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下一代液体活检分析中基于超高频电泳的生物细胞表征和鉴别
本文介绍了利用基于超高频电介质电泳(UHF-DEP)的细胞内传感技术对不同肿瘤细胞系进行表征的结果。在50 MHz以上,本技术允许探测细胞内部含量,以表征其细胞质介电常数和电导率特性。根据细胞类型及其生物学特异性,测量的频率依赖性细胞行为产生独特的DEP特征。本文阐述了超高频实验室芯片传感器在区分来自不同组织的各种肿瘤细胞类型方面的高潜力。
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