Real time computer simulation of transmission electron microscope images with tilted illumination: grain boundary applications.

W Krakow
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引用次数: 4

Abstract

Computer programs have been developed to simulate electron microscope images from digitized graphically represented model structures. Via a television rate image processing system, these programs allow real time, interactive modification of the microscope objective lens parameters, incident beam inclination, and incident beam energy. In addition to explaining the computational methods, the need for using tilted beam illumination is explored to extend microscope resolution. For this study, the subject of grain boundary imaging is analyzed for a copper sigma = 5, 36.9 degrees, (310) tilt boundary with a [001] common rotation axis. The Cu [200] lattice spacings of approximately 1.8A on both sides of the interface cannot be reliably resolved under axial illumination conditions in a 200 kV microscope. Therefore, either tilted beam modes or higher incident beam energies were explored and the types of image features correlated with atomic position data through the digital frame store system.

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倾斜照明下透射电子显微镜图像的实时计算机模拟:晶界应用。
计算机程序已经开发出模拟电子显微镜图像从数字化图形表示的模型结构。通过电视速率图像处理系统,这些程序允许实时,交互式修改显微镜物镜参数,入射光束倾角和入射光束能量。除了解释计算方法外,还探讨了使用倾斜光束照明来扩展显微镜分辨率的必要性。在本研究中,分析了具有[001]共同旋转轴的铜sigma = 5,36.9°(310)倾斜边界的晶界成像对象。在200 kV的轴向光照条件下,不能可靠地分辨界面两侧约1.8A的Cu[200]晶格间距。因此,通过数字帧存储系统,探索倾斜光束模式或更高入射光束能量,以及与原子位置数据相关的图像特征类型。
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Real time computer simulation of transmission electron microscope images with tilted illumination: grain boundary applications. Cryofixation of vascular endothelium. The endothelial vesicle system in cryofixed frog mesenteric capillaries analysed by ultrathin serial sectioning. Lectin and immunolabeling of microvascular endothelia. Quick-freeze, deep-etch studies of endothelial components, with special reference to cytoskeletons and vesicle structures.
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