Evaluation of chemical components of rhizomes and micropropagation from Kaempferia rotunda L.

Abstract

In this study, the extracts of Kaempferia rotunda L. were investigated by GC/MS (Gas chromatography/mass spectrometry). There were 25 compounds in tuberous rhizome: alpha-pinene (4.48%), camphene (20.85%), pentadecane (15.47%), camphor (10.15%), alpha terpinolene (1.01%), bornyl acetate (5.65%), alpha-selinene (2.32%), gamma-curcumene (3.22%), heptadecane (3.80%), alpha-cedrene (3.64%), alpha-amorphene (4.92%), alpha-curcumine (2.68%), benzyl-benzoate (7.56%), eucalyptol (1.01%), and some important other compounds. The protocol for in vitro propagation was conducted. The MS medium supplemented with 2 mg/L of Benzyl adenine and 0.2 mg/L of Kinetin gave the highest number of shoots (4.67 shoots/explant). The MS 1/2 medium supplemented with 0.5 mg/L of Naphthalene acetic acid was suitable for plantlet formation (shoot height: 12.05 cm and 14.56 roots/explant). The greenhouse-acclimated in vitro plants reached a 100% survival rate.