The Effect of Incubation Time on Various Type of Local Agricultural Waste in Madiun, Indonesia to Produce Cellulases Using Trichoderma viride

Abstract

This study aims to determine the effect of substrate types on the crude activity of cellulase enzymes from local agricultural waste such as peanut shells, coconut fibers, brans, and teak leaves with the variations of incubation times (day 1, 3, 5, and 7) produced by Trichoderma viride . Enzyme activity was measured based on the amount of reducing sugar that produced by the DNS (Dinitrosalycilic Acid) method on a wavelength of 540 nm spectrophotometer. The results showed that the best substrate for cellulase production on coconut fibers substrate with an incubation period of 7 days which had the highest enzyme activity value of 1.340 U / ml. Coconut fibers contain the highest cellulose content compared to the other substrates. The lowest activity was shown by coconut fibers substrate at day one incubation time of 0.660 U / ml with the reducing sugar content of 0.594 mg/ml and protein content of 0.147 mg/ml. The complexity of the chemical composition of coconut fibers caused the longer time to degrade cellulose into glucose at day one than other three substrates.