UJI AKTIVITAS SENYAWA ANTIOKSIDAN DARI EKSTRAK DAUN TIIN (Ficus Carica Linn) DENGAN PELARUT AIR, METANOL DAN CAMPURAN METANOL-AIR

KLOROFIL: Jurnal Ilmu Biologi dan Terapan Pub Date : 2017-11-01 DOI:10.30821/kfl:jibt.v1i1.1240

Eva Agustina

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引用次数: 14

Abstract

Antioxidants can ward off free radicals in the body to resist oxidative damage caused by free radicals. The aim of this research is to know the influence of water solvent, methanol and methanol-water mixture to antioxidant activity. The extraction is done by maceration method. The extraction results in the phytochemical test and functional group analysis to determine the compounds contained in Pig leaf extract. Further testing of antioxidant activity with DPPH method. Pig leaf extract with methanol solvent has antioxidant activity with IC50 3,3005 μg / ml value, Pig leaf extract with water solvent has antioxidant activity 3,6976 μg / ml and Pig leaf extract with methanol solvent: water has antioxidant activity 13,6140 μg / ml. The IC50 <50 μg / ml value indicates that the Pig leaf extract with some solvents has potent antioxidant potential. Pig leaf extract with methanol solvent has the best antioxidant activity with IC50 3,3005 μg / ml value because according to phytochemical test and functional group analysis that methanol solvent is able to extract more active compound such as flavonoid, Triterpenoid and Sterol, Alkaloid and Saponin followed by Pig leaf extract with water solvent and methanol mixture: water.

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测试提取物(Ficus Carica Linn)中抗氧化的化合物的活性，以及水溶剂、甲醇和英雄水混合物

抗氧化剂可以抵御体内自由基，抵抗自由基造成的氧化损伤。本研究的目的是了解水溶剂、甲醇和甲醇-水混合物对抗氧化活性的影响。采用浸渍法提取。对猪叶提取物进行植物化学试验和官能团分析，确定其所含化合物。DPPH法进一步测定其抗氧化活性。甲醇溶剂猪叶提取物的抗氧化活性IC50值分别为3,3005 μg / ml，水溶剂猪叶提取物的抗氧化活性为3,6976 μg / ml，水溶剂猪叶提取物的抗氧化活性为13,6140 μg / ml, IC50值<50 μg / ml，说明部分溶剂猪叶提取物具有较强的抗氧化潜力。甲醇溶剂猪叶提取物的抗氧化活性最佳，IC50为3,3005 μg / ml，因为根据植物化学测试和官能团分析，甲醇溶剂能够提取出更多的活性化合物，如黄酮类、三萜和甾醇、生物碱和皂苷，其次是水溶剂和甲醇混合物猪叶提取物:水。

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KLOROFIL: Jurnal Ilmu Biologi dan Terapan

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