Pub Date : 2023-07-11DOI: 10.30821/kfl:jibt.v7i1.13740
Misriani Ani, Kartika Manalu, Rizki Amelia Nasution
One of the traditional plants used by the community is the kapok plant (Ceiba pentandra L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of Pseudomonas aeruginosa and Candida albicans. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the Kirby-Bauer Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against Pseudomonas aeruginosa and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against Candida albicans. The results of the One Way ANOVA test show that Ftable < Fcount which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of Pseudomonas aeruginosa and Candida albicans.
{"title":"Uji Aktivitas Antimikroba Ekstrak Daun Randu (Ceiba pentandra L.) Terhadap Pertumbuhan Pseudomonas aeruginosa dan Candida albicans","authors":"Misriani Ani, Kartika Manalu, Rizki Amelia Nasution","doi":"10.30821/kfl:jibt.v7i1.13740","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.13740","url":null,"abstract":"One of the traditional plants used by the community is the kapok plant (Ceiba pentandra L.) apart from being cultivated, parts of the kapok plant are also used as building materials, stuffing for mattresses and traditional medicine. The purpose of this study was to determine the activity of randu leaf extract on the growth of Pseudomonas aeruginosa and Candida albicans. This study used a completely randomized design (CRD) with 6 treatment groups, namely the positive control group with chloramphenicol and ketoconazole. The negative control group used 10% DMSO, and the extract concentration group was 70%, 80%, 90% and 100%. The steps carried out in this study included the extraction of kapok leaves using 96% ethanol as solvent, phytochemical screening and antimicrobial testing using the Kirby-Bauer Disc Diffusion method. Analysis of research data using SPSS 21. From the results of this study, it was found that randu leaf extract contained alkaloids, saponins and tannins. Antimicrobial testing with the kapok leaf extract group resulted in an average inhibition zone of 7.57 mm, 8.0 mm, 8.7 mm, and 9.87 mm against Pseudomonas aeruginosa and 4.67 mm, 5.07 mm, 5.32 mm, and 5.65 mm against Candida albicans. The results of the One Way ANOVA test show that Ftable < Fcount which proves that the leaf extract of Randu provides antimicrobial activity in inhibiting the growth of Pseudomonas aeruginosa and Candida albicans.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132391753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.30821/kfl:jibt.v7i1.13745
Dita Kurnia Sari
Birds are wild animals that are easy to find in almost every vegetated environment. Its habitat can cover various types of ecosystems, ranging from natural ecosystems to artificial ecosystems, one of which is Situ Cihuni. This research was conducted as an initial step that can be used as a guide for conservation efforts and to determine the balance of aquatic ecosystems. The method used is the field survey method, where the data collected includes the types of birds that are in the transect line and the number of individuals of each bird species found. The results showed that 24 species of birds could be found in Situ Cihuni, with a total encounter of 354 individuals. A diversity index (H') value of 2.23 was obtained, which indicated that the diversity level of bird species in Situ Cihuni was in the moderate category. Then the value of the wealth index (R) is 3.92; the bird with the highest IVI value was the Lonchura punctulata (110.793). Insectivorous bird groups dominate the Situ Cihuni area with a percentage of 46%. The vegetation strata in Situ Cihuni that are most used by birds are strata C and E.
{"title":"Diversitas Burung Di Situ Cihuni","authors":"Dita Kurnia Sari","doi":"10.30821/kfl:jibt.v7i1.13745","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.13745","url":null,"abstract":"Birds are wild animals that are easy to find in almost every vegetated environment. Its habitat can cover various types of ecosystems, ranging from natural ecosystems to artificial ecosystems, one of which is Situ Cihuni. This research was conducted as an initial step that can be used as a guide for conservation efforts and to determine the balance of aquatic ecosystems. The method used is the field survey method, where the data collected includes the types of birds that are in the transect line and the number of individuals of each bird species found. The results showed that 24 species of birds could be found in Situ Cihuni, with a total encounter of 354 individuals. A diversity index (H') value of 2.23 was obtained, which indicated that the diversity level of bird species in Situ Cihuni was in the moderate category. Then the value of the wealth index (R) is 3.92; the bird with the highest IVI value was the Lonchura punctulata (110.793). Insectivorous bird groups dominate the Situ Cihuni area with a percentage of 46%. The vegetation strata in Situ Cihuni that are most used by birds are strata C and E.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131169611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-04DOI: 10.30821/kfl:jibt.v7i1.15378
Emilia Juliyanti Bria
Mangroves are important ecosystems in coastal areas that have a major influence on the survival of the biota in them. This study aims to inventory the existence of mangrove species in the coastal area of Jenilu Village, Kakuluk Mesak District, Belu Regency, East Nusa Tenggara. The method used is the observation method. Data obtained by means of inventory and identification of mangrove species. The results showed that there were 2 mangrove species from the Rhizophoraceae Family, Order Malpighiales, namely Rhizophora apiculata Blum and Rhizophora mucronata Poir. These two species are distinguished based on the characters on their morphological characteristics. The data from this research can become the basic data for species development and subsequent conservation efforts.
{"title":"INVENTARISASI SPESIES MANGROVE DI KAWASAN PESISIR DESA JENILU KABUPATEN BELU NUSA TENGGARA TIMUR","authors":"Emilia Juliyanti Bria","doi":"10.30821/kfl:jibt.v7i1.15378","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.15378","url":null,"abstract":"Mangroves are important ecosystems in coastal areas that have a major influence on the survival of the biota in them. This study aims to inventory the existence of mangrove species in the coastal area of Jenilu Village, Kakuluk Mesak District, Belu Regency, East Nusa Tenggara. The method used is the observation method. Data obtained by means of inventory and identification of mangrove species. The results showed that there were 2 mangrove species from the Rhizophoraceae Family, Order Malpighiales, namely Rhizophora apiculata Blum and Rhizophora mucronata Poir. These two species are distinguished based on the characters on their morphological characteristics. The data from this research can become the basic data for species development and subsequent conservation efforts.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127033678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.30821/kfl:jibt.v7i1.13851
Kiki Nurmayanti
Persamaan dan perbedaan karakter yang dimiliki tanaman padi dapat digunakan untuk mengetahui hubungan kekerabatan yang dimiliki antarvarietas tanaman padi. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan dan jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi. Penelitian dilaksanakan di Kebun percobaan Balai Besar Penelitian Tanaman Padi Sukamandi. Metode penelitian yang digunakan yaitu metode deskriftif eksperimental dengan perlakuan sebanyak 44 varietas padi, diulang 3 kali. Data hasil pengamatan dianalisis menggunakan sofeware Ntsys 2.02 dan 2.11 dengan metode UPGMA. Hasil penelitian menunjukan beberapa varietas unggul terpilih memiliki koefisien kemiripan 0,41 berdasarkan karakter warna lemma steril, warna kepala putik, panjang biji, panjang beras pecah kulit, bentuk beras pecah kulit serta warna kulit ari beras dan terbentuk dua kelompok dengan hubungan kekerabatan yang jauh. Jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi memiliki keragaman yang luas dengan nilai 0,59. Pasangan varietas yang memiliki jarak genetik yang luas diduga akan menghasilkan turunan yang memiliki tingkat keragaman genetik tinggi dan sifat unggul yang lebih dari tetuanya.
{"title":"Hubungan Kekerabatan Beberapa Varietas Unggul Padi (Oryza sativa L) Terpilih Berdasarkan Karakterisasi Kualitas Biji","authors":"Kiki Nurmayanti","doi":"10.30821/kfl:jibt.v7i1.13851","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.13851","url":null,"abstract":"Persamaan dan perbedaan karakter yang dimiliki tanaman padi dapat digunakan untuk mengetahui hubungan kekerabatan yang dimiliki antarvarietas tanaman padi. Penelitian ini bertujuan untuk mengetahui hubungan kekerabatan dan jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi. Penelitian dilaksanakan di Kebun percobaan Balai Besar Penelitian Tanaman Padi Sukamandi. Metode penelitian yang digunakan yaitu metode deskriftif eksperimental dengan perlakuan sebanyak 44 varietas padi, diulang 3 kali. Data hasil pengamatan dianalisis menggunakan sofeware Ntsys 2.02 dan 2.11 dengan metode UPGMA. Hasil penelitian menunjukan beberapa varietas unggul terpilih memiliki koefisien kemiripan 0,41 berdasarkan karakter warna lemma steril, warna kepala putik, panjang biji, panjang beras pecah kulit, bentuk beras pecah kulit serta warna kulit ari beras dan terbentuk dua kelompok dengan hubungan kekerabatan yang jauh. Jarak genetik yang terbentuk pada beberapa varietas unggul terpilih tanaman padi memiliki keragaman yang luas dengan nilai 0,59. Pasangan varietas yang memiliki jarak genetik yang luas diduga akan menghasilkan turunan yang memiliki tingkat keragaman genetik tinggi dan sifat unggul yang lebih dari tetuanya.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121852476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-27DOI: 10.30821/kfl:jibt.v7i1.14589
Fauzan Ahmad Wijaya, Tri Cahyanto
Wawatjan Babad Sumedang is one of the ancient texts telling about the struggle of Prabu Geusan Ulun in defending and taking responsibility for Sumedang from the Cirebon attack as a result of the love between him and Harisbaya, the empress of Cirebon. In one part of this chronicle, the Hanjuang was planted by a character named Embah Djaja Perkosa. It is the background for the study of the manuscript from an ethnobotanical perspective. This study uses qualitative methods with data collection techniques through interviews, observation, and literature studies. Interviews were conducted in a semi-structured and snowball-sampling manner with community leaders. Furthermore, observation was carried out by visiting the Hanjuang Kutamaya complex in Padasuka Village, North Sumedang District, Sumedang Regency, West Java. While the literature study was performed on related references, especially the Wawatjan Babad Sumedang Manuscript. Hanjuang is mentioned in the text in verses 148, 149, 171, and 172. Hanjuang itself is a siloka of "hayu urang berjuang" or "let's fight" . In the written text, the word ngalakai is a sign that the Hanjuang needs an appropriate environment to live in. This suitable environment is filled with the flourishing of Kutamaya thanks to the existence of the river there. In addition, the process of planting Hanjuang in the events in the script is done by stem cuttings. However, the Hanjuang side as an indicator has not been resolved in this study, so further research is needed on this matter
{"title":"Kajian Etnobotani Tanaman Hanjuang (Cordyline fruticosa) pada Wawatjan Babad Sumedang","authors":"Fauzan Ahmad Wijaya, Tri Cahyanto","doi":"10.30821/kfl:jibt.v7i1.14589","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.14589","url":null,"abstract":"Wawatjan Babad Sumedang is one of the ancient texts telling about the struggle of Prabu Geusan Ulun in defending and taking responsibility for Sumedang from the Cirebon attack as a result of the love between him and Harisbaya, the empress of Cirebon. In one part of this chronicle, the Hanjuang was planted by a character named Embah Djaja Perkosa. It is the background for the study of the manuscript from an ethnobotanical perspective. This study uses qualitative methods with data collection techniques through interviews, observation, and literature studies. Interviews were conducted in a semi-structured and snowball-sampling manner with community leaders. Furthermore, observation was carried out by visiting the Hanjuang Kutamaya complex in Padasuka Village, North Sumedang District, Sumedang Regency, West Java. While the literature study was performed on related references, especially the Wawatjan Babad Sumedang Manuscript. Hanjuang is mentioned in the text in verses 148, 149, 171, and 172. Hanjuang itself is a siloka of \"hayu urang berjuang\" or \"let's fight\" . In the written text, the word ngalakai is a sign that the Hanjuang needs an appropriate environment to live in. This suitable environment is filled with the flourishing of Kutamaya thanks to the existence of the river there. In addition, the process of planting Hanjuang in the events in the script is done by stem cuttings. However, the Hanjuang side as an indicator has not been resolved in this study, so further research is needed on this matter","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134442223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-25DOI: 10.30821/kfl:jibt.v7i1.14814
Mustika Sari, Iqbal Maulana
West Kalimantan is an area with a distribution of tropical rain forest in Indonesia which has a fairly hight diversity so that it is estimated to store various types of plants that have the potential as medicinal ingredients. Most of the Dayak Bakati people who live in customary forests in gua village Bengkayang Regency still use medicinal plants as raw materials for traditional medicines which have been passed down from generation to generation and have been going on for a long time. The purpose of this research is to find out the types, parts and ways of processing medicinal plants used by the Dayak Bakati tribe in the customary forest area of Gua Village, Sanggau Ledo District, Bengkayang Regency. Based on the results of interviews and identification in the field with communities living in customary forest areas in Sanggau Ledo District, Bengkayang Regency, there are 28 species and 24 plant families that are used as medicinal plants by the community.
{"title":"PEMANFAATAN TANAMAN OBAT SUKU DAYAK BAKATI DI KAWASAN HUTAN ADAT DESA GUA KECAMATAN SANGGAU LEDO","authors":"Mustika Sari, Iqbal Maulana","doi":"10.30821/kfl:jibt.v7i1.14814","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.14814","url":null,"abstract":"West Kalimantan is an area with a distribution of tropical rain forest in Indonesia which has a fairly hight diversity so that it is estimated to store various types of plants that have the potential as medicinal ingredients. Most of the Dayak Bakati people who live in customary forests in gua village Bengkayang Regency still use medicinal plants as raw materials for traditional medicines which have been passed down from generation to generation and have been going on for a long time. The purpose of this research is to find out the types, parts and ways of processing medicinal plants used by the Dayak Bakati tribe in the customary forest area of Gua Village, Sanggau Ledo District, Bengkayang Regency. Based on the results of interviews and identification in the field with communities living in customary forest areas in Sanggau Ledo District, Bengkayang Regency, there are 28 species and 24 plant families that are used as medicinal plants by the community. ","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130386005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-17DOI: 10.30821/kfl:jibt.v7i1.14059
Nia Agustina, Mauritz Pandapotan Marpaung
Nipah fruticans adalah sejenis palem yang mengandung senyawa metabolit sekunder yang berpotensi memiliki berbagai efek biologis, seperti antibakteri dan antijamur. Penelitian ini bertujuan menganalisis ekstrak akar nipah dapat menghambat pertumbuhan Staphylococcus aureus ATCC 25933 dan jamur Candida albicans ATCC 10231. Akar nipah diekstraksi dengan metode maserasi dalam pelarut etanol 96%. Skrining fitokimia dilakukan untuk mengindentifikasi senyawa metabolit sekunder. Uji mikroba dengan metode Difusi Agar dengan konsenterasi ekstrak 10%, 20%, 30%, 40%, 60%, 80%, dan 100%. Kontrol positif untuk uji antibakteri adalah klindamisin dan ketokonazol untuk uji antijamur. Hasil penelitian yaitu adanya metabolit sekunder berupa flavonoid, alkaloid, saponin, steroid, dan terpenoid. Diameter hambat ekstrak pada uji bakteri terdapat pada ekstrak 100%, sedangkan pada uji jamur, tidak ditemukan adanya daya hambat.
{"title":"Uji Aktivitas Ekstrak Akar Nipah (Nypa fruticans) Sebagai Antimikroba Staphylococcus aureus ATCC 25923 Dan Candida albicans ATCC 10231","authors":"Nia Agustina, Mauritz Pandapotan Marpaung","doi":"10.30821/kfl:jibt.v7i1.14059","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.14059","url":null,"abstract":"Nipah fruticans adalah sejenis palem yang mengandung senyawa metabolit sekunder yang berpotensi memiliki berbagai efek biologis, seperti antibakteri dan antijamur. Penelitian ini bertujuan menganalisis ekstrak akar nipah dapat menghambat pertumbuhan Staphylococcus aureus ATCC 25933 dan jamur Candida albicans ATCC 10231. Akar nipah diekstraksi dengan metode maserasi dalam pelarut etanol 96%. Skrining fitokimia dilakukan untuk mengindentifikasi senyawa metabolit sekunder. Uji mikroba dengan metode Difusi Agar dengan konsenterasi ekstrak 10%, 20%, 30%, 40%, 60%, 80%, dan 100%. Kontrol positif untuk uji antibakteri adalah klindamisin dan ketokonazol untuk uji antijamur. Hasil penelitian yaitu adanya metabolit sekunder berupa flavonoid, alkaloid, saponin, steroid, dan terpenoid. Diameter hambat ekstrak pada uji bakteri terdapat pada ekstrak 100%, sedangkan pada uji jamur, tidak ditemukan adanya daya hambat.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115131511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.30821/kfl:jibt.v7i1.12880
Vila Tria Putri, Rasyidah Rasyidah, Ulfa Mayasari
Amylolytic bacteria have great genetic diversity with high enzymatic activity and have the expertise to break down starch into simpler materials and degrade organic substances in water. This study aims to determine the variety of types of amylolytic bacteria obtained and to see how the characterization of bacteria is isolated from the waters of Paris Tigaras Beach. Isolation of amylolytic bacteria from the waters of Paris Tigaras Beach was carried out using the spread plate method taken from the last dilution of 10-5. Isolates ST3 03 and ST3 04 showed amylolytic activity by showing a clear zone of 11.8 mm in isolates ST3 03 and 8.2 mm in isolates ST3 04. Characterization of isolates ST3 03 and ST3 04 was observed including Gram-positive bacteria with rod-shaped cells, that can produce catalase enzymes, ferment three types of sugars, and move (motil). From the results of observations of the morphology of bacterial colonies and cells, and biochemical tests, isolates of amylolytic bacteria ST3 03 and ST3 04 belong to the genus Bacillus.
{"title":"ISOLASI DAN KARAKTERISASI BAKTERI AMILOLITIK DARI SAMPEL AIR PANTAI PARIS TIGARAS","authors":"Vila Tria Putri, Rasyidah Rasyidah, Ulfa Mayasari","doi":"10.30821/kfl:jibt.v7i1.12880","DOIUrl":"https://doi.org/10.30821/kfl:jibt.v7i1.12880","url":null,"abstract":"Amylolytic bacteria have great genetic diversity with high enzymatic activity and have the expertise to break down starch into simpler materials and degrade organic substances in water. This study aims to determine the variety of types of amylolytic bacteria obtained and to see how the characterization of bacteria is isolated from the waters of Paris Tigaras Beach. Isolation of amylolytic bacteria from the waters of Paris Tigaras Beach was carried out using the spread plate method taken from the last dilution of 10-5. Isolates ST3 03 and ST3 04 showed amylolytic activity by showing a clear zone of 11.8 mm in isolates ST3 03 and 8.2 mm in isolates ST3 04. Characterization of isolates ST3 03 and ST3 04 was observed including Gram-positive bacteria with rod-shaped cells, that can produce catalase enzymes, ferment three types of sugars, and move (motil). From the results of observations of the morphology of bacterial colonies and cells, and biochemical tests, isolates of amylolytic bacteria ST3 03 and ST3 04 belong to the genus Bacillus.","PeriodicalId":142864,"journal":{"name":"KLOROFIL: Jurnal Ilmu Biologi dan Terapan","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128158224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-17DOI: 10.30821/kfl:jibt.v6i2.13582
Erpi Nurdin
Diabetes mellitus is a metabolic disease characterized by hyperglycemia resulting from defects in insulin secretion, insulin action or both. Blood glucose and urine glucose levels will increase in a history of diabetes mellitus, the accumulation of glucose in the urine is a medium for microbes to multiply, which can cause urinary tract infections in patients with diabetes mellitus. Alternative media of tuna extract agar, able to grow bacteria on gram positive and negative bacteria isolates. The aim is to determine the relationship between blood glucose and urine glucose levels on UTI-causing bacterial cultures in urine using tuna alternative media. The research method is descriptive with a comparative approach. Based on the research that has been carried out, the results are described that identified 100% increased blood glucose levels, 63 respondents with high blood glucose levels accompanied by positive results of urine culture on alternative media, and there were 29 respondents with positive urine glucose levels accompanied by positive results of bacterial culture causes of UTI in urine. The conclusion is that an increase in blood and urine glucose can lead to urinary tract infections, even though they are very weak. The p value is 0.036 which states that the correlation between blood glucose levels and the results of UTI-causing bacteria culture in urine using alternative media, namely the strength of the correlation is very weak (0.0 - <0.2). The p value is 0.014 which states that the correlation between urine glucose levels and urine culture results using alternative media, namely the strength of the correlation is very weak (0.0 - <0.2).
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Pub Date : 2023-01-11DOI: 10.30821/kfl:jibt.v6i2.12645
Afina Tur Rohmah
Ulat grayak atau Fall Armyworm (Spodoptera frugiperda J. E. Smith) merupakan hama baru pada tanaman jagung di Indonesia. Hama tersebut berasal dari benua Amerika dan telah menyebar ke berbagai wilayah Afrika dan Asia. Hama ini termasuk sulit untuk dikendalikan, karena penyebaran imago sangat cepat, dan termasuk penerbang yang sangat kuat. Kehilangan hasil yang ditimbulkan oleh kerusakan serangan Spodoptera frugiperda mencapai 40%. Maka diperlukan suatu tindakan untuk mengendalikan hama tersebut salah satunya yaitu dengan menggunakan insektisida. Penelitian ini bertujuan untuk menganalisis pengaruh pemberian insektisida Ammate dengan pemberian dosis yang berbeda terhadap tingkat kematian hama ulat grayak Spodoptera frugiperda pada tanaman jagung. Penelitian ini menggunakan rancangan acak lengkap dengan tiga konsentrasi perlakuan yaitu 5%, 10%, 15% dan satu larutan kontrol yaitu 0%. Data hasil penelitian dianalisis menggunakan aplikasi SPSS versi 22. Hasil penelitian menunjukkan mortalitas yang berbeda pada konsentrasi 0% digunakan sebagai larutan kontrol tidak menunjukkan adanya mortalitas, pada konsentrasi larutan 5% menunjukkan rata-rata mortalitas sebanyak 44%, pada konsentrasi 10% menunjukkan rata-rata mortalitas sebanyak 78%, sedangkan pada konsentrasi larutan 15% menunjukkan rata- rata mortalitas sebanyak 89%, perlakuan dengan konsentrasi larutan 10% dan 15% menunjukkan hasil yang paling signifikan yaitu dapat mematikan 50% dari ulat uji.
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