Panax quinquefolium hairy root extracts and their effect in connections with antibiotics against pathogenic bacteria – preliminary study

Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia Pub Date : 2019-06-10 DOI:10.17951/C.2018.73.1.7-17

E. Kochan

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Abstract

The aim of the present study was to determine the level of ginsenosides in extracts from hairy root A, B, G clones of Panax quinquefolium and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using HPLC. The activity of extracts with antibiotics was established by micro-dilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg^-1 d.w. for root culture clones B, A and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against Enterococcus faecalis and both Escherichia coli and Acintobacter baumannii.

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西洋参毛状根提取物及其与抗生素抗致病菌作用的初步研究

本研究旨在测定西洋参毛状根A、B、G无性系提取物中人参皂苷的含量及其与抗生素对临床分离细菌的作用。采用高效液相色谱法测定了人参皂苷(主要生物活性成分)的含量。采用微稀释肉汤法测定提取物对抗生素的活性。根培养无性系B、A和G的总三萜皂苷含量分别为14.68、14.32和10.07 mg -1 d.w.。我们的研究表明，在抗生素中加入主要来自B和G克隆毛状根培养物的提取物，可以分别降低氨苄西林和四环素对粪肠球菌、大肠杆菌和鲍曼不动杆菌的有效浓度。

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Annales Universitatis Mariae Curie-Sklodowska, sectio C – Biologia

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