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Panax quinquefolium hairy root extracts and their effect in connections with antibiotics against pathogenic bacteria – preliminary study 西洋参毛状根提取物及其与抗生素抗致病菌作用的初步研究
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.7-17
E. Kochan

 

The aim of the present study was to determine the level of ginsenosides in extracts from hairy root A, B, G clones of Panax quinquefolium and their action with antibiotics against clinical bacterial isolates. The content of ginsenosides (the key biologically active compounds) were determined in tested extracts using HPLC. The activity of extracts with antibiotics was established by micro-dilution broth method. Total triterpene saponin content was 14.68, 14.32 and 10.07 mgg-1 d.w. for root culture clones B, A and G, respectively. Our research indicates that the addition of extracts mainly from B and G clone hairy root cultures to antibiotics allow to reduce the ampicillin and tetracycline effective concentration respectively against Enterococcus faecalis and both Escherichia coli and Acintobacter baumannii

本研究旨在测定西洋参毛状根A、B、G无性系提取物中人参皂苷的含量及其与抗生素对临床分离细菌的作用。采用高效液相色谱法测定了人参皂苷(主要生物活性成分)的含量。采用微稀释肉汤法测定提取物对抗生素的活性。根培养无性系B、A和G的总三萜皂苷含量分别为14.68、14.32和10.07 mg -1 d.w.。我们的研究表明,在抗生素中加入主要来自B和G克隆毛状根培养物的提取物,可以分别降低氨苄西林和四环素对粪肠球菌、大肠杆菌和鲍曼不动杆菌的有效浓度。
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引用次数: 0
Diagnosis and treatment of invasive Candida infections – a review article 侵袭性念珠菌感染的诊断和治疗综述
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.47-59
M. Dąbrowska, Monika Sienkiewicz, P. Kwiatkowski, M. Dąbrowski
Candida albicans is the most common cause of fungal infections worldwide. Invasive candidiasis comprises candidemia and deep-seated candidiasis. Most yeast invasive infections are endogenous with a high mortality. Pathogenesis of candidiasis depends on avoiding host immune responses, as well as the virulence factors of the fungus enabling colonization and invasion of tissues. Adequate source control and antifungal therapy administered within a short time is critical to get a better prognosis. The emergence of drug resistance and the side effects of currently available antifungals are becoming the major problem in the management of Candida spp. infection.
白色念珠菌是世界范围内真菌感染的最常见原因。侵袭性念珠菌病包括念珠菌病和深部念珠菌病。大多数酵母菌侵袭性感染是内源性的,死亡率高。念珠菌病的发病机制取决于避免宿主免疫反应,以及真菌的毒力因素,使定植和入侵组织。在短时间内进行充分的源头控制和抗真菌治疗对于获得更好的预后至关重要。耐药性的出现和目前可用的抗真菌药物的副作用正在成为念珠菌感染管理的主要问题。
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引用次数: 1
Ecological and biological aspekt new locality of Drosera anglica Huds. near Końskich 黄颡鱼新居地的生态学和生物学研究。附近Końskich
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.41-46
R. Plackowski
The work concerns the occurrence conditions Drosera anglica. (EN) within the site located 10  km  from the village of au nord   Końskie near   the Czarna River. The species is very dispersed but in a few places the density was 5-10 of specimens per 1m2. This species grows within a patch which is poor in terms of its flora. No significant expansion of trees or shrubs has been noted. This is related to quite a high level of water. Such conditions are favourable to the development of another species under threat of extinction, Rhynchospora alba. Plant-based patches with participation of sundew are heterogeneous in terms of syntaxonomy.  Presence of species has been observed, e.g. from the classes of Phragmitetea and Scheuchzerio-Caricetea nigrae. Soil analyses indicate that the soils under observation are not very acidic, with average content of CaO and ash content which is optimal for this species with the ratio of C:N which indicates a temporary mire. The content of heavy metals is in norm and poses no threat to the surroundings (Pb 64-65 ppm, Cd 2.7-2.9 ppm). Wide ranges in the analysed samples refer to chromium. They do not have an adverse effect on the development of Drosera anglica. A very crucial threat is the consequence of succession, competition and hydrological conditions.
本研究是关于黄鳝发生条件的研究。(EN)位于Czarna河附近au nord Końskie村10公里处的场地内。该物种分布非常分散,但在少数地方密度为每平方米5-10个标本。这个物种生长在植物区系贫乏的一小块土地上。没有注意到树木或灌木的显著扩张。这与高水位有关。这样的条件有利于另一种濒临灭绝的物种——白纹蛛的发展。藓属植物参与的斑块在分类学上具有异质性。已经观察到物种的存在,例如从Phragmitetea和Scheuchzerio-Caricetea nigrae类。土壤分析表明,观察到的土壤酸性不强,平均CaO含量和灰分含量对该物种来说是最佳的,C:N比表明是暂时的泥潭。重金属含量(Pb 64 ~ 65 ppm, Cd 2.7 ~ 2.9 ppm)在正常范围内,对周围环境无威胁。在分析的样品中,较宽的范围是指铬。它们对鹅毛癣的发展没有不良影响。一个非常关键的威胁是演替、竞争和水文条件的后果。
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引用次数: 0
Assessing accuracy of barley yield forecasting with integration of climate variables and support vector regression 基于气候变量和支持向量回归的大麦产量预测精度评价
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.19-30
L. Parviz
Investigations of the relation between crop yield and climate variables are crucial for agricultural studies and decision making related to crop monitoring. Multiple linear regression (MLR) and support vector regression (SVR) are used to identify and model the impact of climate variables on barley yield. The climate variables of 36 years (1982–2017) are gathered from three provinces of Iran with different climate: Yazd (arid), Zanjan (semi-arid), Gilan (very humid). Air temperature by high correlation coefficient with barley yield was introduced as the dominant climate variable. According to evaluation criteria, SVR provided accurate estimation of crop yield in comparison with MLR. The diversity of climate impressed the estimated yield in which UI, decreasing from Gilan to Yazd provinces, was 47.77%. Support vector machine (SVM) with capturing the nonlinearity of time series, could improve barley yield estimation, with the minimum UI for Yazd province. Also, the minimum correlation coefficient between the observed and simulated yield was found in Gilan province. Based on GMER calculations, SVM forecasts were underestimated in three provinces. All findings show that SVM is able to have high efficiency to model the climate effect on crop yield.
研究作物产量与气候变量之间的关系对农业研究和与作物监测有关的决策至关重要。采用多元线性回归(MLR)和支持向量回归(SVR)对气候变量对大麦产量的影响进行识别和建模。36年(1982-2017)的气候变量来自伊朗三个不同气候的省份:亚兹德(干旱)、赞詹(半干旱)、吉兰(非常湿润)。引入与大麦产量具有高相关系数的气温作为优势气候变量。根据评价标准,SVR比MLR更能准确估计作物产量。从吉兰到亚兹德省,气候多样性影响了作物产量的估算,其UI值为47.77%。利用支持向量机(SVM)捕捉时间序列的非线性特征,可以提高大麦产量的估计精度,对Yazd省的预测精度最小。此外,吉兰省的观测产量与模拟产量之间的相关系数最小。基于GMER计算,SVM预测在三个省份被低估。结果表明,支持向量机能够高效地模拟气候对作物产量的影响。
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引用次数: 4
Usnea jørgenseniana Bystr. & Leśniewska sp. nova Usnea (subgen. Usnea, Parmeliaceae) in Sweden
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.31-39
Jan Bystrek, Joanna Leśniewska
Usnea jørgenseniana Bystr. & Leśniewska sp. nova, an epixilic species of bushy Usnea (Parmeliaceae) in Sweden, is similar to U. hirta var. minutissima (Mer.) Bystr., but the similarity to U. hirta is apparent. U. jørgenseniana is not a species from the foveatae Mot. section. The lack of soralia and a very small thallus (0.3–2.0 cm) makes it difficult to locate U. jørgenseniana in the section Comosae Mot. It colonizes exceptionally unfavorable climatic conditions, a coprophilous species. Collected by G. Ohrstedt in 1937. Dozens of specimens from one position.
Usnea jørgenseniana Bystr。& Leśniewska sp. nova,瑞典浓密的Usnea (Parmeliaceae)的一种附生种,类似于U. hirta var. minutissima (Mer.)。Bystr。但与美国的相似之处是显而易见的。uj ørgenseniana不是一个来自中央凹形的物种。部分。在Comosae Mot剖面中,由于缺少伞柄和很小的菌体(0.3-2.0 cm),很难确定U. jørgenseniana的位置。它在极端不利的气候条件下定居,是一种嗜粪物种。1937年G. Ohrstedt收藏。几十个标本来自一个位置。
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引用次数: 0
Diagnosis and treatment of mucosa Candida spp. infections – a review article 粘膜念珠菌感染的诊断和治疗综述
Pub Date : 2019-06-10 DOI: 10.17951/C.2018.73.1.61-68
M. Dąbrowska, Monika Sienkiewicz, P. Kwiatkowski, M. Dąbrowski
Candida albicans is the most common cause of fungal infections worldwide. Non-albicans Candida species play an important role in vulvovaginal candidiasis and invasive infections. Most cases of infections are endogenous. In case of patients with immune disorders this opportunistic pathogen causes both surface, systemic infections, and candidemia. Symptoms depend on the area affected. Candidiasis are treated with antimycotics; these include clotrimazole, nystatin, fluconazole, voriconazole, amphotericin B, and echinocandins. The emergence of drug resistance and the side effects of currently available antifungals are becoming a major problem in the management of Candida spp. infection.
白色念珠菌是世界范围内真菌感染的最常见原因。非白色念珠菌在外阴阴道念珠菌病和侵袭性感染中起重要作用。大多数感染病例是内源性的。在患有免疫系统疾病的患者中,这种机会性病原体可引起表面、全身感染和念珠菌病。症状取决于受影响的区域。用抗真菌药物治疗念珠菌病;这些药物包括克霉唑、制霉菌素、氟康唑、伏立康唑、两性霉素B和棘白菌素。耐药性的出现和目前可用的抗真菌药物的副作用正在成为念珠菌感染管理的主要问题。
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引用次数: 4
Morphophysiological and biochemical response of savory medicinal plant using silicon under salt stress 盐胁迫下含硅咸味药用植物的形态生理生化响应
Pub Date : 2019-01-07 DOI: 10.17951/C.2017.72.2.29-40
H. Mohammadi, S. Hazrati, L. Parviz
Salt stress is one of the most important factors limiting the growth and yield of plants around the world. However, silicon can reduce the harmful effects of salt stress on plants. For this purpose, an experiment was conducted in a factorial arrangement on randomized complete block design with three replications in a research greenhouse on the Satureja hortensis medicinal plant. Experimental treatments consisted of two salinity levels (control and 100 mM) and potassium silicate (Si) at three levels (0, 1, and 2 mM). The results showed that salinity reduced shoot dry weight, photosynthetic pigments and potassium content of shoot. However, sodium, proline, MDA, and H2O2 contents in shoot increased. The highest shoot dry weight, photosynthetic pigment content, proline, RWC, and the lowest content of MDA and H2O2 of the shoot were observed with Si application under salt stress and non-salt stress conditions. The highest yield of essential oil was also observed with Si application under salt stress and non-salt stress conditions. Therefore, the use of silicon in salt stress condition not only minimizes the harmful effects of salt stress by increasing the K+/Na+ ratio and improving the morphological and physiological traits of the Satureja hortensis medicinal plant but also improves the essential oil yield of this medicinal plant in salt stress and non-salt stress conditions.
盐胁迫是世界范围内限制植物生长和产量的最重要因素之一。然而,硅可以减少盐胁迫对植物的有害影响。为此,本试验采用随机完全区组设计,采用三次重复的因子设计,在研究温室中对竹属药用植物进行了试验。试验处理包括两个盐度水平(对照和100 mM)和三个盐度水平(0、1和2 mM)的硅酸钾(Si)。结果表明,盐度降低了地上部干重、光合色素和钾含量。但茎部钠、脯氨酸、丙二醛和H2O2含量增加。盐胁迫和非盐胁迫条件下,施硅处理的地上部干重、光合色素含量、脯氨酸、RWC最高,MDA和H2O2含量最低。在盐胁迫和非盐胁迫条件下,施硅可获得最高的精油产量。因此,在盐胁迫条件下施用硅不仅可以通过提高盐胁迫下的K+/Na+比值,改善石斛药用植物的形态和生理性状,最大限度地减少盐胁迫的有害影响,还可以提高石斛药用植物在盐胁迫和非盐胁迫条件下的精油产量。
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引用次数: 1
Fascinating fructophilic lactic acid bacteria associated with various fructose-rich niches 迷人的亲果糖乳酸菌与各种富含果糖的生态位有关
Pub Date : 2019-01-07 DOI: 10.17951/C.2017.72.2.41-50
A. Pachla, A. Ptaszyńska, Magdalena Wicha, Ewa Oleńska, W. Małek
Fructophilic lactic acid bacteria (FLAB) are recently described group of lactic acid bacteria (LAB) that prefer fructose instead of glucose as a carbon source. FLAB have been isolated from fructose-rich niches such as flowers, fruits, fermented fruits, and gastrointestinal tracts of insects whose diet is based on fructose. These bacteria are divided into obligate and facultative fructophilc lactobacilli based on biochemical features. All FLAB are heterofermentative microorganisms, which during fermentation of carbohydrates, in addition to lactic acid, produce also acetic acid, and alcohol as end-products. The fructophilic bacteria, inhabiting the honeybee guts positively impact the health of their hosts, improve their longevity, and are promising probiotic candidates. These symbionts of honeybees play a key role in the production of honey by bees and are present in a large number in fresh honey. The combination of osmolarity with antibacterial, and therapeutic properties of these bacteria make fresh honey optimal alternative for future wound healing.
嗜果糖乳酸菌(FLAB)是近年来发现的一类以果糖代替葡萄糖作为碳源的乳酸菌。FLAB已从富含果糖的生态位中分离出来,如花、水果、发酵水果和以果糖为主食的昆虫的胃肠道。这些细菌根据其生化特征分为专性和兼性嗜果糖乳酸杆菌。所有FLAB都是异发酵微生物,在碳水化合物发酵过程中,除乳酸外,还产生醋酸和酒精作为最终产物。栖息在蜜蜂肠道中的嗜果糖细菌对宿主的健康有积极的影响,可以延长宿主的寿命,是有希望的益生菌候选者。这些蜜蜂的共生体在蜜蜂生产蜂蜜中起着关键作用,在新鲜蜂蜜中大量存在。这些细菌的渗透性与抗菌和治疗特性的结合使新鲜蜂蜜成为未来伤口愈合的最佳选择。
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引用次数: 8
Genotypic response of barley to exogenous application of nanoparticles under water stress condition 水分胁迫条件下大麦对纳米颗粒外源施用的基因型响应
Pub Date : 2019-01-07 DOI: 10.17951/C.2017.72.2.15-27
H. Ghorbanian, M. Janmohammadi, Asghar Ebadi-Segherloo, N. Sabaghnia
Beneficial nanoparticles (SiO2 and TiO2) can have various profound effects on the crop physiological, biochemical and morphological characteristics. Here, we evaluated the mitigation of drought stress in barley genotypes by foliar application of SiO2 and TiO2 nanoparticles under filed condition in North West of Iran. Nanoparticles were foliar applied in late vegetative phase and during reproductive stages. Drought was imposed at by irrigation withdrawals during the dry months in the end of the growing season. We measured parameters related morphological growth, yield, and yield component. The genetic diversity between the genotypes was quite evident and the highest seed yield and yield component were recorded for G1, G2, G4, G11, G12 and G13. Foliar application of nanoparticles considerably affected the plant height, thousand seed weight, biological and seed yield. The best performance was observed for plant treated with SiO2 nanoparticles. Spike length of G2, G6, G13 and G20 considerably responded to nano silicone foliar application. However, the best results for G8, G11 and G20 were obtained by foliar application TiO2 nanoparticles while this treatment decreased the seed yield components in G1, G5, G9, G10, G15 and G20. This could be due to genetic variation between the evaluated genotypes and high sensitivity of some genotypes to the applied concentration. The results of current study showed that application of SiO2 nanoparticles under water stress condition could have more beneficial effects on yield component of barley genotypes.
有益的纳米颗粒(SiO2和TiO2)对作物的生理、生化和形态特征具有各种深远的影响。本文研究了在伊朗西北部大田条件下,叶面施用SiO2和TiO2纳米颗粒对大麦基因型干旱胁迫的缓解作用。在营养后期和生殖期叶面施用纳米颗粒。干旱是由于在生长季节结束的干旱月份里,灌溉被撤回而造成的。我们测量了与形态生长、产量和产量成分相关的参数。基因型间遗传多样性显著,G1、G2、G4、G11、G12和G13的种子产量和产量构成最高。叶面施用纳米颗粒对植株高度、千粒重、生物产量和种子产量均有显著影响。结果表明,SiO2纳米颗粒处理的植物性能最好。G2、G6、G13和G20的穗长对纳米硅叶面施用有显著的响应。但在G8、G11和G20中,叶面施用TiO2纳米颗粒效果最好,而在G1、G5、G9、G10、G15和G20中,TiO2纳米颗粒处理降低了种子产量成分。这可能是由于评估基因型之间的遗传差异以及某些基因型对施用浓度的高敏感性。本研究结果表明,在水分胁迫条件下施用SiO2纳米颗粒对大麦基因型的产量成分有更有利的影响。
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引用次数: 10
Effects of Forchlorofenuron (CPPU) treatment on fruit properties in the fruit of common guava 氟氯非脲(CPPU)处理对普通番石榴果实特性的影响
Pub Date : 2019-01-07 DOI: 10.17951/C.2017.72.2.7-14
Z. Sabaghnia, F. Z. Nahandi
The common guava is a small tree from Myrtacaeae family which is cultivated for its fruits. Researches have explored new methods to promote fruit yields and quality of crops. Application of Forchlorofenuron or CPPU (with Molecular Formula: C12H10CIN3O) improves the fruit size as well as its quality, but there has not been an investigation evaluating its effects on common guava fruit under field conditions. This research was performed to study the effects of different doses of CPPU (0, 10, 20 and 40 mg L-1) on common guava fruit size and quality characteristics under field conditions. Analysis of variance and LSD (least significant differences) mean compression indicated that total soluble solids, total acidity, ascorbic acid or vitamin C, fruit firmness, phenolics, 1,1-Diphenyl-2-picrylhydrazyl (DPPH), polygalacturonase, pectin methyl esterase and ethylene were significantly different in most traits and CPPU-40 produced high means. The principal components (PC) analysis explained 95% of the total variation and the first two principal components (PC1 and PC2) explained 78% and 17% of the total variation, respectively. According to biplot, CPPU-40 had the highest values for all of the measured traits except DPPH, ethylene and polygalacturonase. The most prominent relations by biplot were a strong positive correlation among phenolics, fruit firmness, total acidity, total soluble solids and ascorbic acid as indicated by the small obtuse angles between their vectors. The measured traits were grouped into two clusters and cutoff point verified via Wilks’ lambda statistics. Cluster I consisted of three traits (ascorbic acid or vitamin C, fruit firmness and ethylene) while cluster II included total soluble solids. Findings of this study suggest that CPPU can be used as an effective growth regulator to improve the size and quality of common guava fruit.
常见的番石榴是桃金娘科的一种小树,因其果实而种植。研究人员探索了提高果实产量和作物品质的新方法。施用氯虫腈或CPPU(分子式:c12h10cin30o)可以改善番石榴果实的大小和品质,但在田间条件下对普通番石榴果实的效果尚未见研究。本研究在田间条件下,研究不同剂量CPPU(0、10、20和40 mg L-1)对普通番石榴果实大小和品质特性的影响。方差分析和LSD(最不显著差异)均值压缩表明,可溶性固定性、总酸度、抗坏血酸或维生素C、果实硬度、酚类物质、1,1-二苯基-2-苦酰肼(DPPH)、聚半乳糖醛酸酶、果胶甲基酯酶和乙烯在大多数性状上存在显著差异,CPPU-40产生了高均值。主成分(PC)分析解释了95%的总变异,前两个主成分(PC1和PC2)分别解释了78%和17%的总变异。双标图显示,除DPPH、乙烯和聚半乳糖醛酸酶外,CPPU-40在所有性状中均最高。双图显示酚类物质与果实硬度、总酸度、总可溶性固形物和抗坏血酸之间呈明显的正相关关系。将测量的性状分为两类,并通过Wilks λ统计验证截断点。聚类I包括三个性状(抗坏血酸或维生素C、果实硬度和乙烯),聚类II包括总可溶性固形物。本研究结果表明,CPPU可作为一种有效的生长调节剂,改善普通番石榴果实的大小和品质。
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引用次数: 0
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