Conteúdo de bioativos e atividade antioxidante em cultivares de feijão-Caupi antes e após o cozimento

N. V. D. A. Barros, Bruna Barbosa de Abreu, Marcos Antônio de Araújo, M. D. M. Rocha, R. S. D. R. Moreira-Araújo
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Abstract

This study evaluated the content of bioactive compounds and antioxidant activity in four genetically improved varieties of cowpea, before and after steaming in pot of domestic pressure. The phenolic compounds were analyzed and flavonoids by spectrophotometry, anthocyanins by the pH difference method, total flavanols by the method of vanillin, polyamines by high-performance liquid chromatography, and the antioxidant activity by the method of capture of the free radical DPPH and ABTS. The BRS Aracê phenolic compounds showed the highest content (mg/100 g) prior to (205.10 + 2.89) and after (150.62 + 2.64) cooking (p<0.05). They were identified and quantified polyamines spermine and spermidine (mg / kg), highlighting the BRS Milênio (raw-120.5; cooked50.4) and BRS Tumucumaque (raw-116.2; baked-47.9) with loss after cooking (p<0.05). For the antioxidant activity were observed different behaviors for each cultivar in both methods. It was concluded that, even after heat processing, cultivars maintained relevant levels of bioactive compounds. PALAVRAS-CHAVE: Vigna unguiculata (L.) Walp.; compostos bioativos; atividade antioxidante; processamento térmico.
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豇豆品种蒸煮前后的生物活性含量和抗氧化活性
本研究对4个遗传改良豇豆品种在家用压力锅蒸熟前后的生物活性物质含量和抗氧化活性进行了评价。采用分光光度法分析酚类化合物和黄酮类化合物,用pH差法分析花青素,用香兰素法分析总黄酮醇,用高效液相色谱法分析多胺,用捕获自由基DPPH和ABTS法分析抗氧化活性。BRS Aracê酚类化合物含量以蒸煮前(205.10 + 2.89)和蒸煮后(150.62 + 2.64)最高(mg/100 g) (p<0.05)。鉴定并定量了多胺精胺和亚精胺(mg / kg),突出显示BRS Milênio (raw-120.5;烹煮50.4)和BRS Tumucumaque(生-116.2;烘焙-47.9),蒸煮后损失(p<0.05)。两种方法对不同品种的抗氧化活性有不同的测定结果。综上所述,即使经过热处理,品种的生物活性成分仍保持在一定水平。PALAVRAS-CHAVE: Vigna unguiculata (l)Walp。compostos bioativos;atividade antioxidante;processamento termico。
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