Study on Rapid Optical Measurement Method and Device of Hemoglobin Concentration

Abstract

In order to achieve rapid and accurate measurement of hemoglobin concentration in human whole blood, a rapid optical detection system was developed. The measurement principle, optical path design, mechanical structure design and microcuvette design are studied. Firstly, the rapid measurement of hemoglobin concentration was studied by using the scattering and spectral absorption characteristics of erythrocytes. Secondly, the optical path design and structure design of the optical measurement system are analyzed and studied. Then, a microcuvette for optical detection is designed, and its structure is designed according to fluid mechanics, and its precision is analyzed according to measurement error. Finally, the calibration curve and precision analysis of the measurement system, as well as the correlation analysis with the standard High iron cyanide process method are given. The experimental results show that the system can give the hemoglobin concentration results within 3 seconds, the CV value is less than 2%, the correlation coefficient is greater than 0.99, and the correlation with the international standard cyanidation high-speed iron method can reach more than 0.99.The detection device has the characteristics of high speed, high precision, no pollution in the detection process, and high efficiency and environmental protection.