A comparison of the physical and biochemical properties of NAD-dependent glyceraldehyde-3-phosphate dehydrogenases from three lactic acid bacteria

Abstract

The glyceraldehyde-3-phosphate dehydrogenases from Streptococcus faecalis, Lactobacillus acidophilus and Pediococcus cerevisiae were purified to homogeneity and characterized. The respective enzymes exhibit estimated molecular weights of 135,000, 155,000 and 152,000. The enzymes appear to be composed of four subunits of identical size ranging from a molecular weight of 37,000 to 42,000. Preliminary immunodiffusion experiments with anti P. cerevisiae glyceraldehyde-3-phosphate dehydrogenase serum indicate that the enzymes share some degree of structural homology.