A micro cell lysis device

Proceedings MEMS 98. IEEE. Eleventh Annual International Workshop on Micro Electro Mechanical Systems. An Investigation of Micro Structures, Sensors, Actuators, Machines and Systems (Cat. No.98CH36176 Pub Date : 1998-01-25 DOI:10.1109/MEMSYS.1998.659798

Sang-Wook Lee, H. Yowanto, Y. Tai

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引用次数: 210

Abstract

A new micromachined cell lysis device is developed. It is designed for miniature bio-analysis systems where cell lysing is needed to obtain intracellular materials for further analysis such as DNA identification. It consists of multi-electrode pairs to apply electric fields to cells. We adopt the means of using electric field lysing because it call greatly simplify purification steps for preparation of biological samples, when compared to conventional chemical methods. Yeast, Chinese cabbage, radish cells and E. Coli are tested with the device. The lysis of yeast, Chinese cabbage, radish cells is observed by a microscope. The experimental observation suggests E. Coli are also lysed by the pulsed electric field. The range of electric field for the lysis is on the order of 1 kV/cm to 10 kV/cm. In addition, for practical reasons, we reduce the voltage required for lysing to less than 10 V by making the electrode gap on the order of microns.

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一种微细胞裂解装置制造方法及图纸

研制了一种新型微机械细胞裂解装置。它是为微型生物分析系统而设计的，其中需要细胞裂解以获得细胞内物质以进行进一步分析，如DNA鉴定。它由多电极对组成，用于向细胞施加电场。我们采用电场裂解的方法，因为与传统的化学方法相比，它大大简化了制备生物样品的纯化步骤。用该设备对酵母、白菜、萝卜细胞和大肠杆菌进行了测试。用显微镜观察酵母、白菜、萝卜细胞的裂解过程。实验观察表明，脉冲电场对大肠杆菌也有裂解作用。用于裂解的电场范围约为1kv /cm至10kv /cm。此外，出于实际原因，我们通过使电极间隙在微米量级上，将裂解所需的电压降低到10 V以下。

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Proceedings MEMS 98. IEEE. Eleventh Annual International Workshop on Micro Electro Mechanical Systems. An Investigation of Micro Structures, Sensors, Actuators, Machines and Systems (Cat. No.98CH36176

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