Spider Crossopriza lyoni Purified Venom Toxin Induced Bio-molecular Alterations in Blood of Albino Mice

Abstract

: In the present investigation biomolecules in the blood of spider Crossopriza lyoni were determined in albino mice. For visualizing biomolecule effects, physiological or sub-lethal dose of purified venom toxins of spider Crossopriza lyoni was administered in laboratory reared albino mice. Level of serum total protein was decreased significantly (p<0.05) up to 78 % and 69 % at 6 h of 40% and 80% of 24-h LD 50 , respectively as compared to control. Later on, it was slightly recovered up to 85 % and 84 % at 10 h in comparison to control. Level of serum free amino acid was increased significantly (p<0.05) up to 117 % at 6 h of 40% of 24-h LD 50 of purified Crossopriza lyoni venom toxins while 80 % of 24-h LD 50 caused a significant increase of 151 % at 8 h. A significant (p<0.05) elevation in serum uric acid was found to be 138 % and 136 % at 8 h of treatment with 40 % and 80 % of 24-h LD 50 of purified Crossopriza lyoni venom toxins, respectively in comparison to control. Similarly a marginal reduction in serum cholesterol level i.e. 87 % and 88 % was found at 10 h of treatment with 40 % and 80 % of 24-h LD 50 of purified venom toxins from C. lyoni in comparison to control. Serum pyruvic acid increased significantly (p<0.05) up to maximum 164 % at 6 h of treatment with 40 % of 24-h LD 50 of purified C. lyoni venom toxins in comparison to control. Similarly, a significant (p<0.05) elevation in serum glucose level i.e. 152 % was noted at 10 h of treatment with 40 % of 24-h LD 50 of purified C. lyoni venom toxins in comparison to control.