Isolation and identification of some types of histamine-producing bacteria from shrimp in local markets

Abstract

Different genera and species of bacteria were isolated from shrimp, which were brought from the Al-Faw area in the Basra governorate, and these isolates were diagnosed after purification through studying their phenotypic characteristics and conducting API confirmation, biochemical tests, motility examination and staining with gram stain, in addition to using different types of diagnostic tools bacteria. Twenty-five bacterial isolates of genera and species of bacteria were isolated and diagnosed from shrimp. Morganella morganii sub. Morganii,, Klebsiella pneumonia sub. Pneumonia, Proteus marina, Enterobacter cloacae, Klebsiella oxytoca, Enterobacter aerogenes, Proteus mirabilis, Proteus vulgaris, Enterobacter tailored, Cronobacter sakazakii, Escherichia coli, Hafnia alvei, Citrobacter amalonaticus, Citrobacter freundii, Vibrio harveyi, Vibrio vulnificus, Serratia marcescens, Pseudomonas aeruginosa, Staphylococcus intermedius, Staphylococcus saprophyticus, Pseudomonas fluorescens, Bacillus subtilis, Bacillus licheniformis, Staphylococcus epidermidis, Aeromonas sobria. The results of the detection of histamine-producing bacteria, which were detected by using broth media such as Histamine decarboxylase broth (HDB), Trypticase Soy brothhistidine (TSBH) and solid media such as Histamine decarboxylase agar (HDA) showed that all bacteria were producing histamine in different proportions except Aeromonas sobria and Hafnia alvei were non-histamine-producing. Keywords: histamine, shrimp, bacterial contamination