{"title":"Functionalization of Carbon Nanotubes with Antibodies for Breast Cancer Detection Applications","authors":"R. Sirdeshmukh, K. Teker, B. Panchapakesan","doi":"10.1109/ICMENS.2004.74","DOIUrl":null,"url":null,"abstract":"We study the effect of functionalization of Carbon Nanotubes (CNTs) with a primary monoclonal mouse immunoglobin G (IgG) specific to the cell-surface receptors of breast cancer cells, and secondary polyclonal goat ant- mouse IgG. The CNTs, in solution with a surfactant (sodium dodecyl benzene sulfonate) were labeled with dihexyloxacarbocyanine iodide (DiOC6), a fluorescent dye, in order to view them with fluorescently labeled antibodies through confocal microscopy. Co-localization for CNTs in combination with the primary antibody conjugated to the secondary was determined to be 90%, whereas CNTs in combination with the secondary antibody and polyethylene glycol (PEG), a polymer used to block CNTs from proteins binding to their surface, was found to be very minimal (0.5%). Preliminary studies on the electrical measurements of the primary mouse IgG incubated with CNTs show a decrease in conductance compared to that of bare CNT field effect transistors (CNTFETs). This observed change in conductance, can eventually be amplified and utilized in applications leading to a full-fledged breast cancer detection system in the future.","PeriodicalId":344661,"journal":{"name":"2004 International Conference on MEMS, NANO and Smart Systems (ICMENS'04)","volume":"78 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2004-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"14","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2004 International Conference on MEMS, NANO and Smart Systems (ICMENS'04)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICMENS.2004.74","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 14
Abstract
We study the effect of functionalization of Carbon Nanotubes (CNTs) with a primary monoclonal mouse immunoglobin G (IgG) specific to the cell-surface receptors of breast cancer cells, and secondary polyclonal goat ant- mouse IgG. The CNTs, in solution with a surfactant (sodium dodecyl benzene sulfonate) were labeled with dihexyloxacarbocyanine iodide (DiOC6), a fluorescent dye, in order to view them with fluorescently labeled antibodies through confocal microscopy. Co-localization for CNTs in combination with the primary antibody conjugated to the secondary was determined to be 90%, whereas CNTs in combination with the secondary antibody and polyethylene glycol (PEG), a polymer used to block CNTs from proteins binding to their surface, was found to be very minimal (0.5%). Preliminary studies on the electrical measurements of the primary mouse IgG incubated with CNTs show a decrease in conductance compared to that of bare CNT field effect transistors (CNTFETs). This observed change in conductance, can eventually be amplified and utilized in applications leading to a full-fledged breast cancer detection system in the future.