Graphene Conductive Polymer Paper-Based Biosensor for Interleukin-6 Detection in Human Serum

Md Ashiqur Rahman, Nazmul Islam, Robert Freeman, Ali Ashraf
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Abstract

Abstract Biosensing technology has advanced significantly in recent years because of its wide applications in healthcare and biomolecule detection. However, fabricating a biosensor that will be rapid, sensitive, inexpensive, simple, and selective can be challenging. In this study, we fabricated a paper-based biosensor using conductive polymers (CPs) and graphene to detect Interleukin-6 (IL-6) in human serum. Cross-sectional scanning electron microscopy (SEM) was carried out and the images indicated that flat graphene nanoflake (GNF) sheets are connected to the fibrous structure of cellulose. We used 20 μL of human serum and 2 μL of IL-6 antigen concentration to measure the electrical impedance spectroscopy (EIS) using Autolab potentiostat (PGSTAT302N). A typical three-electrode method was preferred over the two-electrode method for better accuracy, and the sensor was connected through an adapter to the potentiostat. The paper-based biosensor detected in the range of 2 pg/mL to 20 μg/mL (the solution was prepared by serial dilution method), and the charge transfer resistance (Rct) increases linearly with the concentration (899 Ω–964 Ω). The limit of detection (LOD) of IL-6 detection was ∼16 pg/mL for our biosensor. Thus, using a portable, simple, sensitive, and inexpensive paper-based biosensor can be a great platform to detect early-stage cancer biomarkers.
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基于石墨烯导电聚合物纸的人血清白细胞介素6检测生物传感器
摘要近年来,生物传感技术因其在医疗保健和生物分子检测方面的广泛应用而取得了长足的进步。然而,制造一种快速、灵敏、廉价、简单和有选择性的生物传感器是具有挑战性的。在这项研究中,我们利用导电聚合物(CPs)和石墨烯制作了一种纸基生物传感器来检测人血清中的白细胞介素-6 (IL-6)。横断面扫描电子显微镜(SEM)图像显示,扁平的石墨烯纳米片(GNF)与纤维素的纤维结构相连。取20 μL人血清和2 μL IL-6抗原浓度,用Autolab电位器(PGSTAT302N)测量电阻抗谱(EIS)。典型的三电极法比两电极法精度更高,传感器通过适配器连接到恒电位器。纸基生物传感器检测范围为2 pg/mL ~ 20 μg/mL(溶液采用串联稀释法制备),电荷传递电阻(Rct)随浓度线性增加(899 Ω-964 Ω)。该生物传感器的IL-6检测限(LOD)为~ 16 pg/mL。因此,使用便携、简单、灵敏、廉价的纸质生物传感器可以成为检测早期癌症生物标志物的一个很好的平台。
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