Dielectrophoretic Movement of Cell Passing Between Surface Electrodes in Flow Channel

Shigehiro Hashimoto, Ryuya Ono
{"title":"Dielectrophoretic Movement of Cell Passing Between Surface Electrodes in Flow Channel","authors":"Shigehiro Hashimoto, Ryuya Ono","doi":"10.1115/1.4063463","DOIUrl":null,"url":null,"abstract":"Abstract In this study, cell behavior in a microchannel was tracked for the application of dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was fabricated inside the microchannel by photolithographic techniques: a triangular electrode with a tip angle of 0.26 rad and a rectangular electrode. A periodic alternating current of square wave with a period of 1 μs was introduced between the electrodes to induce an asymmetric electric field perpendicular to the mainstream direction. The behavior of mouse myoblasts (C2C12: mouse myoblast cell line) was measured in vitro while the suspension was flowing. The relationship between cell shift motion near the electrode and cell shape on the two-dimensional projection plane was investigated. Experimental results showed that cell movement in the direction perpendicular to the mainstream increased with geometries away from the circle in the two-dimensional projection plane. This method can be applied to sort cells according to their degree of shape deviation from a sphere. The dielectrophoretic effect can be applied to sort cells not only by cell size but also by cell deformation.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"87 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of engineering and science in medical diagnostics and therapy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1115/1.4063463","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Abstract In this study, cell behavior in a microchannel was tracked for the application of dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was fabricated inside the microchannel by photolithographic techniques: a triangular electrode with a tip angle of 0.26 rad and a rectangular electrode. A periodic alternating current of square wave with a period of 1 μs was introduced between the electrodes to induce an asymmetric electric field perpendicular to the mainstream direction. The behavior of mouse myoblasts (C2C12: mouse myoblast cell line) was measured in vitro while the suspension was flowing. The relationship between cell shift motion near the electrode and cell shape on the two-dimensional projection plane was investigated. Experimental results showed that cell movement in the direction perpendicular to the mainstream increased with geometries away from the circle in the two-dimensional projection plane. This method can be applied to sort cells according to their degree of shape deviation from a sphere. The dielectrophoretic effect can be applied to sort cells not only by cell size but also by cell deformation.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
流动通道中细胞在表面电极间传递的介电泳运动
摘要本研究对微通道中的细胞行为进行了跟踪,以便将介质电泳技术应用于生物细胞分选。采用光刻技术在微通道内制备了一对钛表面电极:一个尖端角为0.26 rad的三角形电极和一个矩形电极。在电极间引入周期为1 μs的方波交流电,产生垂直于主流方向的不对称电场。小鼠成肌细胞(C2C12:小鼠成肌细胞系)在体外流动时的行为。研究了细胞在电极附近的移动运动与细胞在二维投影平面上的形状之间的关系。实验结果表明,在二维投影平面上,当几何形状远离圆时,细胞在垂直于主流方向上的运动增加。这种方法可以根据细胞的形状偏离球体的程度来进行分类。介电泳效应不仅可以应用于细胞大小,还可以应用于细胞变形。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
High-Speed Three-Dimensional-Digital Image Correlation and Schlieren Imaging Integrated With Shock Tube Loading for Investigating Dynamic Response of Human Tympanic Membrane Exposed to Blasts. Quantifying the Fascicular Changes in Recovered Achilles Tendon Patients Using Diffusion Magnetic Resonance Imaging and Tractography. Assistive Technology for Real-Time Fall Prevention during Walking: Evaluation of the Effect of an Intelligent Foot Orthosis A Simple Poc Device for Temperature Control of Multiple Reactions During Recombinase Polymerase Amplification Auxetic Structure Inspired Microneedle Arrays for Minimally Invasive Drug Delivery
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1