Sex determination of papaya var. ‘Maradol’ reveals hermaphrodite-to-male sex reversal under greenhouse conditions

IF 1.3 4区农林科学 Q3 AGRONOMY Crop Breeding and Applied Biotechnology Pub Date : 2023-01-01 DOI:10.1590/1984-70332023v23n3a35

José Guadalupe Ávila-Hernández, Alberto Camas-Reyes, Agustino Martinez-Antonio

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引用次数: 1

Abstract

Papaya is a nutritious fruit cultivated worldwide under suitable climate conditions. This plant is polygamous, bearing female, male, and hermaphrodite sex types determined by sex chromosomes XX, XY, and XYh, respectively. In this paper, a molecular sex determination of papaya var. ‘Maradol’ was carried out based on PCR and specific primers. Specific molecular markers resulted in sixty-nine hermaphrodites and twenty-one female plants, matching 100% to the flower morphology. Nevertheless, since the summer stressing conditions rose in the greenhouse, sex reversal to male phenotype was observed in 43% of hermaphrodite plants due to high-temperature conditions. A specific male marker could not detect that change, supporting the proposal that sex reversal is caused by harsh environmental conditions aimed at epigenetic modification and genes related to hormones. Our study demonstrates the effectiveness of molecular sex determination and the importance of controlling the growing requirements of papaya to avoid sex reversal.

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木瓜品种‘Maradol’的性别测定揭示了在温室条件下雌雄同体到雄性的性别逆转

木瓜是一种营养丰富的水果，在适宜的气候条件下在世界各地种植。这种植物是一夫多妻的，有雌性、雄性和雌雄同体的性别类型，分别由性染色体XX、XY和XYh决定。利用PCR和特异引物对番木瓜品种‘Maradol’进行了分子性别鉴定。特异分子标记结果显示雌雄同体69株，雌花21株，与花形态100%匹配。然而，由于温室内夏季胁迫条件的增加，43%的雌雄同体植物由于高温条件而出现了雄性表型的性别逆转。一种特定的男性标记无法检测到这种变化，这支持了性别逆转是由旨在表观遗传修饰的恶劣环境条件和与激素相关的基因引起的这一说法。我们的研究证明了分子性别决定的有效性，以及控制木瓜生长需求以避免性别逆转的重要性。

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来源期刊

Crop Breeding and Applied Biotechnology AGRONOMY-BIOTECHNOLOGY & APPLIED MICROBIOLOGY

CiteScore

2.70

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.