Simvastatin retards cartilage degradation and subchondral bone deterioration induced by a high-fat diet in mice

IF 2.1 4区 农林科学 Cyta-Journal of Food Pub Date : 2023-09-15 DOI:10.1080/19476337.2023.2245008
Hetong Li, Faming Tian, Yu Gou, Yunpeng Hu, Qiangqiang Lian, Liu Zhang
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Abstract

To evaluate the effects of simvastatin on the degeneration of cartilage and subchondral bone in a mice model of osteoarthritis (OA) induced by obesity. Thirty male 3-month-old C57BL/6J mice were randomized into three groups: mice with normal diet + vehicle (distilled water) (Control), mice with High-fat diet + vehicle (HFD), mice with HFD + Simvastatin (HFD+S). HFD+S group were treated with simvastatin (10 mg/kg/day) for 12 weeks. The pathology of OA was assessed by histomorphology analyses, immunohistochemistry, micro-computed tomography, and enzyme-linked immunosorbent assay. Histomorphological analysis revealed that OA was significantly exacerbated by the HFD-induced obesity and markedly alleviated by the simvastatin intervention. In details, simvastatin ameliorated the abnormal metabolic status and cartilage lesions, significantly increased aggrecan and collagen-II expression and decreased the expression of MMP-13 and leptin in cartilage. Furthermore, the results of micro-CT analysis revealed that the HFD+S group exhibited higher BMD, BV/TV, and Tb.N values but a lower Tb.Sp value than that of the HFD group. Serum glucose, leptin, and IL-1β concentrations were significantly correlated with the OARSI score. Histomorphological analysis revealed that OA was significantly exacerbated by the HFD-induced obesity and markedly alleviated by the simvastatin intervention. In details, simvastatin ameliorated the abnormal metabolic status and cartilage lesions, significantly increased aggrecan and collagen-II expression and decreased the expression of MMP-13 and leptin in cartilage. Furthermore, the results of micro-CT analysis revealed that the HFD+S group exhibited higher BMD, BV/TV, and Tb.N values but a lower Tb.Sp value than that of the HFD group. Serum glucose, leptin, and IL-1β concentrations were significantly correlated with the OARSI score. The HFD-induced obesity aggravates articular degeneration and deterioration in subchondral bone, which could be improved by the intervention of simvastatin, suggesting that simvastatin may be a potential candidate for amelioration of the progression of obesity induced-OA.
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辛伐他汀延缓小鼠高脂肪饮食诱导的软骨退化和软骨下骨退化
探讨辛伐他汀对肥胖性骨关节炎(OA)小鼠软骨及软骨下骨退行性变的影响。将30只3月龄雄性C57BL/6J小鼠随机分为正常饮食+载药(蒸馏水)组(对照组)、高脂饮食+载药组(HFD)、高脂饮食+辛伐他汀组(HFD+S)。HFD+S组给予辛伐他汀(10 mg/kg/天)治疗,疗程12周。通过组织形态学分析、免疫组织化学、显微计算机断层扫描和酶联免疫吸附试验评估OA的病理。组织形态学分析显示,hfd诱导的肥胖显著加重了OA,辛伐他汀干预明显减轻OA。辛伐他汀改善了异常代谢状态和软骨病变,显著增加了软骨中aggrecan和collagen-II的表达,降低了软骨中MMP-13和leptin的表达。此外,micro-CT分析结果显示,HFD+S组表现出更高的BMD、BV/TV和Tb。N值,但Tb值较低。Sp值高于HFD组。血清葡萄糖、瘦素和IL-1β浓度与OARSI评分显著相关。组织形态学分析显示,hfd诱导的肥胖显著加重了OA,辛伐他汀干预明显减轻OA。辛伐他汀改善了异常代谢状态和软骨病变,显著增加了软骨中aggrecan和collagen-II的表达,降低了软骨中MMP-13和leptin的表达。此外,micro-CT分析结果显示,HFD+S组表现出更高的BMD、BV/TV和Tb。N值,但Tb值较低。Sp值高于HFD组。血清葡萄糖、瘦素和IL-1β浓度与OARSI评分显著相关。hfd诱导的肥胖加重了关节退变和软骨下骨退化,辛伐他汀干预可以改善这一情况,提示辛伐他汀可能是改善肥胖诱导oa进展的潜在候选药物。
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来源期刊
Cyta-Journal of Food
Cyta-Journal of Food FOOD SCIENCE & TECHNOLOGY-
CiteScore
4.40
自引率
0.00%
发文量
37
期刊介绍: CyTA – Journal of Food is an Open Access journal that publishes original peer-reviewed research papers dealing with a wide range of subjects which are essential to the food scientist and technologist. Topics include: chemical analysis of food; additives and toxins in food; sensory, nutritional and physiological aspects of food; food microbiology and biotechnology; changes during the processing and storage of foods; effect of the use of agrochemicals in foods; quality control in food; and food engineering and technology.
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